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Noob here-2 attempts w c10's cheesecloth agar filtering
    #29676302 -

I finally made a new account... long story-pardon my new account if that's a concern...
(my previous account was named horribly.)

OK so, been at this for years very passively...

ppl say making agar is comparable to making Jelo-Shots?

I built an entire flowhood, prob like a thousand dollars...I would like to  to see I guess if at least this thing is relatively sterile by testing w plates before germinating/inoculating plates.
anyways def not a entry level easy thing generally speaking...

I have honestly had some hard time figuring things out here like:
why can't i see any way to change my mood on edit my  profile?

Where should I ask this question?

hopefully it's ok for me to ask here.
if not pls help direct me...specifically instead of just telling me "main"..am I on main cultivation sub now? idek... if I go to agar subforum it seems dead...


Questions:
  • 1. Does the agar mix (agar-agar+LME+water) need to be boiled before straining through cheesecloth?
  • 2. Is my Cheesecloth too fine? I do also have wayy less fine cheesecloth available to use alternatively, & some ppl don't strain at all I think?..(however, it did work first time so I know my cheesecloth can strain just fine when I boil the mix together well enough to brown pot first)
  • 3. LME I think at bottom of plate? I have never seen anyone say you need to shake the media bottle up before pouring into plates...but I am a noob to agar. My (overcooked) plates from first attempt won't show any signs of growth on them-with LME it shouldn't matter as much but maybe they don't have enough nutes..more likely they just got too hot and lost too much moisture (if heat retention in glass really is as big of a problem as I've gathered)my media bottles have a bunch of LME bits that don't blend in so it's not exactly crystal clear though it would be it not for what I assume is that stuff floating around (after straining through cheesecloth).


I'm using agar-agar powder packets
-ingredients list it as just agar-agar
I am also using LME bags from a brew store...

overall I think I followed c10s guide pretty closely

1st attempt:
...My pot was turning brown at bottom when boiling/simmering mix...then I think my glass "media bottle" was hot for too long bc I let PC sit for too long I guess after PCing first time around...I think the heat of the glass over time maybe overcooked the media as well? glass thermo-insulation is the reason I don't want to do no-pour with glass plates/dishes after reading some else's issue)
I went out for several hrs and open my PC when I came home.

2nd attempt:
this time I was worried about browning the pot so, I just heated it up until everything seemed combined-but I assume ofc this was my mistake on attempt #2. Then (after PCing for 20 mins and allowing to cool for about 25 mins) I opened my PC (in flow hood) and took media bottles out and waited about 25 more mins before I poured them, just letting the flowhood run on them - but they never started to solidify...just stayed liquid..when I was straining the cheesecloth I picked up a lot of, like, granular gel...I assume this was the unboiled agar-agar..I tried to re-boil the mix a little and re-add the agar but, every time I strained it seemed like the agar wouldn't pass and would be strained (remaining outside of the media bottle stuck way up in the cheesecloth/funnel part)..
with a gloved hand I even tried to force the stuff through the cheesecloth but it felt like cheap rubber bouncy ball material.

So next time I will boil everything properly but I'm worried about burning it, and hoping that if all goes right that I can put it in my flowhood and it will be clean there until I'm ready to use it..and also that the nutes are mixed in well..


any help appreciated.
Sorry if this is not the right way to post or anything


--------------------
son of a donkey

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Re: Noob here-2 attempts w c10's cheesecloth agar filtering [Re: shoulderchips]
    #29676347 -

Hi, fellow noob here, someone else would be able to give you better advice, and feel free to correct me if i'm misleading here.

I don't think filtering agar with a cheesecloth is standard procedure, atleast not in any TEK i've read :sunny:

What works well for me is, i simmer the agar and water in a pot on medium to high heat. hard boil is unnessecary, atleast has been for me.

then straight from pot into media bottle, unscrew lid a quarter turn, foil the top and pc at 15psi for around 30 mins.

i dont think shaking the bottle is necessary, though i have been swirling it around a lil before pouring.

i happen to have a laser thermometer, makes the whole thing so much easier when you have a temp to go off.

good luck and have fun! :mushroom2:


--------------------
I wish you bountiful fruits and godspeed :heart:

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Re: Noob here-2 attempts w c10's cheesecloth agar filtering [Re: Mashedpotatoes]
    #29676352 -

if all I did wrong was not boil before PCing that would be great. I do want to cheesecloth strain/filter though but anyways

if that's all I did wrong and my assessment is basically accurate, then I should be safe to keep making more attempts..I don't get on Shroomery a lot but, based on this first reply I feel like I'm good to go for attempt #3
(without fear of wasting time and resources)

will check back here ASAP. thanks! :smile:


--------------------
son of a donkey

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Re: Noob here-2 attempts w c10's cheesecloth agar filtering [Re: shoulderchips]
    #29676360 -

I'm gonna look more into cheeseclothing agar i guess :rasta: I think it's got to be as dissolved as possible beforehand or you'll filter out the agar and it won't solidify properly im guessing.

But hell yeah, go get that agar my guy :rockon:


--------------------
I wish you bountiful fruits and godspeed :heart:

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Re: Noob here-2 attempts w c10's cheesecloth agar filtering [Re: Mashedpotatoes] * 1
    #29676368 -

The agar is probably clogging up your cheese cloth. I get some DME chunks in my agar. It doesn't cause any problems, but if you wanted to get rid of it, why not filter the DME/water first, allow it to cool completely, then just add the agar and bring it to boil?

Kloakk mentioned that they do their DME/water first and let it sit in the fridge for a while to settle. They then just pour off the clear stuff on top and discard the chunks

I do want to stress that you don't have to filter the mixture unless you're going for perfectly clear plates


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Noob here-2 attempts w c10's cheesecloth agar filtering [Re: PuffM4gicDr4gon]
    #29676372 -

PuffM4gicDr4gon said:
The agar is probably clogging up your cheese cloth. I get some DME chunks in my agar. It doesn't cause any problems, but if you wanted to get rid of it, why not filter the DME/water first, allow it to cool completely, then just add the agar and bring it to boil?

Kloakk mentioned that they do their DME/water first and let it sit in the fridge for a while to settle. They then just pour off the clear stuff on top and discard the chunks

I do want to stress that you don't have to filter the mixture unless you're going for perfectly clear plates



:whathesaid:

I don't let my DME(dry malt extract) mix sit in my fridge, but I do it with grain water.

For DME plates I:

Mix DME and RO or distilled water in a pot, stir most of the time while it's heating up. When it reaches boil I turn down to a light boil and let it boil 5 minutes, I then run the mix through a coffee filter(not needed if you don't care about clarity) and then add the mix back in the pot.

Add agar agar and stir while it comes back up to a boil, when it's boiling I turn the heat off and transfer it to a media bottle and PC for 25 minutes.

Wait until it's cooled to about 60°C(140°F) and pour plates, you can let it cool more if you're not doing a lot of plates at once.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Noob here-2 attempts w c10's cheesecloth agar filtering [Re: Kloakk]
    #29676724 -

Kloakk said:
PuffM4gicDr4gon said:
[... why not filter the DME/water first, allow it to cool completely, then just add the agar and bring it to boil?

Kloakk mentioned that they do...then just pour off the clear stuff on top and discard the chunks

...you don't have to filter the mixture unless you're going for perfectly clear plates]



:whathesaid:

I don't let my DME(dry malt extract) mix sit in my fridge, but I do it with grain water.

For DME plates I:

Mix DME and RO or distilled water in a pot, stir most of the time while it's heating up. When it reaches boil I turn down to a light boil and let it boil 5 minutes, I then run the mix through a coffee filter(not needed if you don't care about clarity) and then add the mix back in the pot.

Add agar agar and stir while it comes back up to a boil, when it's boiling I turn the heat off and transfer it to a media bottle and PC for 25 minutes.

Wait until it's cooled to about 60°C(140°F) and pour plates, you can let it cool more if you're not doing a lot of plates at once.



Oh my goodness thank you guys so much!
I can't decide if I will strain w/ a cheesecloth going forwards but I think I will (also strain agar-agar solution with cheesecloth) as I tbh would ideally like it as close to crystal-clear as possible..

My issue has been heating up the mix/solution in the pot with the agar, so I think I will do the malt extract ( I use LME) first and heat, and I don't even need to weigh the agar bc they've always been exactly on weight ime (comes out to exactly to weight printed/stated on ea little baggy, so) then I'll just drop my agar-agar into the pot whenever or however I think it suitable.

Kloakk said:
...when it's boiling I turn the heat off...



I think this is/was my main concern and is what caused me to over-correct on attempt #2...I didn't and still don't know exactly how much to boil (and what all complications can be involved in this step).

So many ppl say they simply don't want it to boil over (in microwave).
I don't have microwave sized media bottles so I boil in super clean stainless steel pots, leaving me to wonder whether I over-cooked it as it doesn't boil over on me in the pot ime..just started browning on bottom of pot when boiling and sticking to the sides of the pot too! :mad:
I really tried not to burn it but by the time it started to boil it was already burned..:frown:

maybe if I wouldn't have left my media bottle slowly cooling in my PC for 5 hours it wouldn't have messed up (on attempt #1)
(my first set of dishes just don't seem to want to show any germination/contamination even though I'm coughing on them..maybe need to observe for longer...)

like cooking in general, this seems to take some practice on it's own (outside of playing with agar/LME/ratio)
I will try to boil it again without burning it...
I'm very unsure what to really think/do specifically regarding this bc I'm not exactly certain what I'm going for..
but I feel that if attempt #2  would have solidified, it would have been perfect-they looked clear although they had too little agar

(attempt/batch #1 are cloudy-not inoculating by contrast)


........thinking more outloud here actaully...maybe I'll just simply microwave it in clean mason jars so I don't have to worry anymore about burning the agar-agar lol...
does burning/overcooking make it cloudy for others?
Anyways
never seen others discuss issues I've had-sorry if I didn't search well enough...kind of surprised no one had any similar issues with c10's agar guide on his post replies but then again, I'm a noob.
 
I will update honestly-I swear I won't forget to get back on here and report whether I'm able to fix it and share findings.
thanks again and sorry for being so detailed-I always hope my experience might be relevant/helpful for others.


--------------------
son of a donkey

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Re: Noob here-2 attempts w c10's cheesecloth agar filtering [Re: shoulderchips]
    #29676837 -

Don't strain or filter the solution after you add agar agar. When you say LME I guess you mean light malt extract in powder form? If that's the case then it's LDME. LME is liquid malt extract, in the brewing world anyway :lol:

If you decide to microwave it be aware that even if you watch it closely it can create air pockets that will pop and make a mess that sucks to clean up :uhoh:

Stir the whole time while you bring it to a boil in a pot and it won't burn or turn dark.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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