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Is this contam? LC and agar
    #29658574 -

LC has been inoculated. Agar has not.






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Re: Is this contam? LC and agar [Re: Thought Criminal]
    #29658590 -

Sorry, but yes and yes.
If you inoculated the LC with spores, most likely contaminated.
If you haven't yet inoculated your agar and you have growth, most definitely contaminated.


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The Combo Tek - Easy grow method for newbies and pros alike

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Re: Is this contam? LC and agar [Re: veggie]
    #29658594 -

:whathesaid:


--------------------
"Why try for high fives,
When my pies have nine lives?"

JOIN CAKE WARS III TODAY

PSA : MODERN FRUITING CONDITIONS (CUBES)

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Re: Is this contam? LC and agar [Re: veggie]
    #29658620 -

veggie said:
Sorry, but yes and yes.
If you inoculated the LC with spores, most likely contaminated.
If you haven't yet inoculated your agar and you have growth, most definitely contaminated.


Ok thanks

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Re: Is this contam? LC and agar [Re: Thought Criminal]
    #29658738 -

How did you prepare your agar? So that possible failure points can be identified.

Edited by CreonAntigone (09/01/26 03:32 AM)

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Re: Is this contam? LC and agar [Re: CreonAntigone]
    #29658832 -

The agar looks bacterial. Likely insufficient sterilization.


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Re: Is this contam? LC and agar [Re: Pastywhyte]
    #29659692 -

Spotty plate is a good indicator of bad technique.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Re: Is this contam? LC and agar [Re: DV8DUG]
    #29659749 -

DV8DUG said:
Spotty plate is a good indicator of bad technique.


If it was mold or yeast yes. Bacteria is rarely airborne tho. Those might be yeast but usually yeast looks dry to me invitro.


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Re: Is this contam? LC and agar [Re: Pastywhyte]
    #29659779 -

Pastywhyte said:
DV8DUG said:
Spotty plate is a good indicator of bad technique.


If it was mold or yeast yes. Bacteria is rarely airborne tho. Those might be yeast but usually yeast looks dry to me invitro.


I used to get bacterial satellites when I bumped my arm in the SAB :uhoh:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Is this contam? LC and agar [Re: Kloakk]
    #29659785 -

Kloakk said:
Pastywhyte said:
DV8DUG said:
Spotty plate is a good indicator of bad technique.


If it was mold or yeast yes. Bacteria is rarely airborne tho. Those might be yeast but usually yeast looks dry to me invitro.


I used to get bacterial satellites when I bumped my arm in the SAB :uhoh:


Are you sure they were bacteria? I guess if you were scattering skin cells everywhere when you bumped it maybe bacteria could hitch a ride there. But I've never had bacteria in that particular scenario myself.

Edit: these are the things I should really break the scope out for. Maybe one day I'll be proficient enough with it to do so.


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Re: Is this contam? LC and agar [Re: Pastywhyte]
    #29659788 -

Pastywhyte said:
Are you sure they were bacteria? I guess if you were scattering skin cells everywhere when you bumped it maybe bacteria could hitch a ride there. But I've never had bacteria in that particular scenario myself.


I can't be 100% sure. But yes I would like scrape my arm against the SAB armhole going in :lol:

These guys:



--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Is this contam? LC and agar [Re: Kloakk]
    #29659798 -

Kloakk said:
Pastywhyte said:
Are you sure they were bacteria? I guess if you were scattering skin cells everywhere when you bumped it maybe bacteria could hitch a ride there. But I've never had bacteria in that particular scenario myself.


I can't be 100% sure. But yes I would like scrape my arm against the SAB armhole going in :lol:

These guys:




When I got patterns like that, it was from when I sprayed liquids into the sab.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Is this contam? LC and agar [Re: LadysKnight]
    #29659802 -

LadysKnight said:
When I got patterns like that, it was from when I sprayed liquids into the sab.


It might come from not waiting long enough for the alcohol on my gloves to evaporate as well, I still pressume it's bacteria though, but again not sure, I'm sure it ain't from spraying because I always used my SAB dry.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Is this contam? LC and agar [Re: LadysKnight]
    #29659805 -

That is a lot going on there, the diversity of species in there confounds the vector somewhat. I'm not sure i could say exactly what was going on there.


--------------------
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Re: Is this contam? LC and agar [Re: Pastywhyte]
    #29659808 -

Yeah, op you gotta research about sterile techniques first and foremost


--------------------
"Why try for high fives,
When my pies have nine lives?"

JOIN CAKE WARS III TODAY

PSA : MODERN FRUITING CONDITIONS (CUBES)

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Re: Is this contam? LC and agar [Re: Pastywhyte] * 1
    #29659809 -

Pastywhyte said:
That is a lot going on there, the diversity of species in there confounds the vector somewhat. I'm not sure i could say exactly what was going on there.


Uhm well that is because what you're looking at is a small portion of my pot with leftover agar I forgot about for a couple of days :rofl:



--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Is this contam? LC and agar [Re: Kloakk] * 1
    #29659839 -

It looks like fallout 5


--------------------
"Why try for high fives,
When my pies have nine lives?"

JOIN CAKE WARS III TODAY

PSA : MODERN FRUITING CONDITIONS (CUBES)

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Re: Is this contam? LC and agar [Re: langten69]
    #29659846 -

I guess the big question for me on this one is were the plates no pour? I kinda assumed he was doing no pour but now I realize that was an assumption. Would be good if he could clarify that as if it was no pour then it's 99% likely insufficient sterilization and is bacteria. If he poured that so.ehow it could be a number of vectors; shitty technique, insufficient sterilization, or the cups were not sterile.


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Re: Is this contam? LC and agar [Re: langten69]
    #29659854 -

langten69 said:
It looks like fallout 5


:rofl::rofl::rofl:


Pastywhyte said:
I guess the big question for me on this one is were the plates no pour? I kinda assumed he was doing no pour but now I realize that was an assumption. Would be good if he could clarify that as if it was no pour then it's 99% likely insufficient sterilization and is bacteria. If he poured that so.ehow it could be a number of vectors; shitty technique, insufficient sterilization, or the cups were not sterile.


It's condiment cups, so he must have poured them.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Is this contam? LC and agar [Re: Kloakk] * 1
    #29659874 -

Okay forgive my boomerness. I had no idea pouring condiment cups was common practice now. That shit is sus af to me, I would not even consider that a viable method. Are they at least sterilizing them beforehand?

Apologies again, I was out of my depth. Crazy how things change.


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