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Pan Cyan LC--->Sterilized bulk thoughts on substrate
    #29634260 -

I've had some early success with pan cyans.  I used the substrate from the Monotub 3.0 tek. It's a manure/coir recipe, and seemed to do ok. (although I am most certainly not an expert--I was happy it worked at all).

I've been very interested in Asura's method (based on the Blue Helix) method of innoculating sterilized bulk with LC.  The substrates were something like 2 parts HP, 1 part chopped straw, some verm, a little gypsum.  And a "supplemental grain".

I have some chopped straw, but it seems to me using a little coir helps the mycellium run more easily as it's not trying to jump between lengths of straw.

So that's my current plan--a substrate made of HP, straw, coir, verm, and gypsum supplemented with some hydrated grain.

My basic plan is to hydrate the manure with a significant xs of water generating more of a manure slurry/tea, then using that to hydrate the coir.  that should really help evenly distribute the manure through the rest of the substrate.

The idea was to hydrae the components separately, so that each could have the field capacity dialed in. I can tweak it a little bit with water or verm at the end.

One question I had was, what would be a good starting point for the ratio of supplemental grain?  Spawning to bulk, it looks like a lot of people go 1:2 or so, but the high ratio seems to be an issue of colonization speed.  Since I would be going to sterilized bulk, I'm curious if I had to go that high.  If I prepared, say, 6 quarts of the above bulk substrate, would 1 quart of grain work? (I've not decided yet--I have oats, popcorn, wbs, and brown rice.  I'm thinking about going with brown rice--at least on BR agar, the pans fly through it).

Any thoughts on the matter?  I did a lot of reading, and because it's hard to know what "1 quart of straw" is (because of packing, the length it's cut...), that the substates can end up all over the place.  But also, cultivators claim it isn't critically important.  If it is more manure or straw heavy, the pans don't seem to care too much.

I'm just wondering if there are any critical parameters here.  Cubes can be fruited from straight grain, or diluted quite a bit into bulk substrate.  Do pans have some parameters where they will or won't fruit? Too much grain or not enough?

I have everything I need. Bags, syringes. I tested my LCs on agar and pint grain jars, and no signs of anything nasty after 5 days. I'm certain it's clean.

I think the trick here, using a variety of components, would be ensuring field capacity was correct.  The straw would help structure and hold some water. The coir and verm would hold a ton of water as well as nutrients from the manure, and the grain would be the ultimate nutrition source for the mycellium.  Give it all a generous PC cycle, and it should be good to go.

I've been intimidated to work with pans, as my earlier understanding was that they were extremely tempermental with respect to temperature, humidity, and FAE. But some of the more modern methods seem to imply they are not THAT tricky. In fact, the Monotub tek suggested actually misting the surface, pins and fruits and all.  Several other vids and readings also didn't do anything fancy with respect to foggers, fans, etc. A couple used a simple air line from an aquarium pump through the side of the tub, and just had it kick on a few minutes every hour.

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Re: Pan Cyan LC--->Sterilized bulk thoughts on substrate [Re: John in WI]
    #29634404 -

With AIO's and especially with bags I think being a little under field capacity is better, often they get a lot of condensation and that will pool at the bottom of the bag unless you break and shake often, I have more success with grains to pasteurized substrate then AIO bags, but that could just be me :lol:

With AIO's I don't think it matters what grains you use really.

As for grain ratio I almost always use 1:4 AIO and grains to pasteurized substrate, pans doesn't seem to digest grains easily even though they colonize them fast. Strawboy have been using LI to pasteurized substrate without any grains at all and tree frog have fruited pans directly from cased grains, so a 1:6 grain ratio should be fine.

Pans can be grown in tubs or automated setups, I do get better results in my dreamers then in ez dial tubs, but pans being very potent you will get enough fruits either way. Bisporus already likes being misted so I find them easier to fruit in tubs. Like Baba Yaga writes in his pan tub tek thread, some cultures don't like misting and working with clones and culture until you find one that don't mind misting is best to get good results.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Pan Cyan LC--->Sterilized bulk thoughts on substrate [Re: Kloakk]
    #29634895 -

Thanks for the tips. I'll have to read up on Strawboy and Tree Frog's methods.  I'm going on some old info/teks.  The one I really studied was from Asura, which was where the idea of LC to sterilized bulk came from. They claimed it was the fastest inoculation to fruit.  I had been dealing with a spell of bad contamination, so I liked the idea of keeping everything sterile until the very end, then putting the fully colonized grain/bulk substrate in trays. 

I'm going to work something out and try to hit it over the weekend. I have bags, syringes, and I tested my pan LC, which is not showing anything nasty after 5 days.

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Re: Pan Cyan LC--->Sterilized bulk thoughts on substrate [Re: John in WI]
    #29635124 -

John in WI said:
Thanks for the tips. I'll have to read up on Strawboy and Tree Frog's methods.  I'm going on some old info/teks.  The one I really studied was from Asura, which was where the idea of LC to sterilized bulk came from. They claimed it was the fastest inoculation to fruit.  I had been dealing with a spell of bad contamination, so I liked the idea of keeping everything sterile until the very end, then putting the fully colonized grain/bulk substrate in trays. 

I'm going to work something out and try to hit it over the weekend. I have bags, syringes, and I tested my pan LC, which is not showing anything nasty after 5 days.


Asura's method is solid and I have had success doing it almost exactly like he describes in his write up so it's not that it isn't going to work, it will, I just find that grains to pasteurized substrate has done better for me. That could easily just be the fact that I only grow pans during summer and the temperature will flocculate a lot in my grow space and that I need to get good with bags :lol:

If you have the time and space you can always do both and see what makes most sense to you. I have also done colonized grains to steril substrate in bags and that will work as well, grains to bags in a SAB is a PITA though :rofl:

Either way, good luck and hope you keep us posted :rockon:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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