jee3apha said:

So I decided to try some transfers, all of them using the trench method (first attempt). I basically transferred from 3 of the previous plates:



Follows pictures of the used donor plates.

From one of the first row, I tried to transfer just the halo:



From one of the second row I tried to transfer just a wedge of the border:



From one of the third row I tried to transfer just some ropes of mycelium using a pair of tweezers:



Notice how in the meantime, the halo started looking less organized, as if it started to turn into the tomentose white mycelium on top...

I also kept one of the plates as is, just to see what happens, and how the halo will evolve.

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Sorry for veering the conversation away from Dave /s but here are some updates after 6 days since the above transfers.

Here's one of the plates from the most rhizomorphic one (third picture above) from which I transferred just one mycelium rope at a time using a pair of tweezers, you can see the mycelium successfully move past the trench. This is my first time doing the trench, now while I think this is pretty OK, in hindsight I could've dug a longer trench, comments are most welcome. Anyway I'll transfer again in the next days.



Transfers from other donor plates only displays some random tomentose mycelium, nothing worth to report at this stage. The only thing to notice is that the halo seems to have now turned into that tomentose mycelium. And this is confirmed by the plate that I decided to keep after the transfer (IIRC top right plate in the first image above).



As you can see the halo really turned into some tomentose mycelium, hence I'd say that the halo was just an intermediate phase at this time, for whatever reason. About it being cubensis mycelium or some other mold I'm not sure, even though I'd lean towards the former... what do you think?

jee3apha said:

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if thats cube myc? im going to just assume those are trash(contam). maybe im wrong but if i had those id trash and focus on better looking/healthier myc.

jee3apha said:

The only thing to notice is that the halo seems to have now turned into that tomentose mycelium. And this is confirmed by the plate that I decided to keep after the transfer (IIRC top right plate in the first image above).

...

As you can see the halo really turned into some tomentose mycelium, hence I'd say that the halo was just an intermediate phase at this time, for whatever reason. About it being cubensis mycelium or some other mold I'm not sure, even though I'd lean towards the former... what do you think?

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Dave Bennett said:

I'd take the next transfer from the sector at 3 o'clock in your second pic as it looks slightly more uniform than the rest of the plate.

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mushboy said:

jee3apha said:

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if thats cube myc? im going to just assume those are trash(contam). maybe im wrong but if i had those id trash and focus on better looking/healthier myc.

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Rasmodius said:

This happened to a bunch of mine too, about 50/50. Some of them are clearly wispy contam and were never myc, but the ones that fleshed out look half decent, so IDK what to think either.

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Rasmodius said:

I've got a shitload of plates related to this project, so it's hard to keep track of individual ones. But when I was unsure earlier, I put a bunch of them under the microscope and marked the ones that had that reaching, more angular, slightly thicker seeming mycelium. Many of the marked plates that had distinct halos and were questionable early on have now fluffed up well, and become more orderly, to where they seem much more like tomentose cube myc than either of the mold presentations I've been running into lately.

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Quote:

It's still possible that it's two organisms on top of each other, [...]

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Dave Bennett said:

Thank you for veering the conversation away from me! I think the halo was cube mycelium and it looks like it's making much better growth on the new plate. I'd take the next transfer from the sector at 3 o'clock in your second pic as it looks slightly more uniform than the rest of the plate.

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You mean the recessed part of the edge where it looks like it might have run up against a contam?

Take this guy’s (chat gpt) advice at your own risk.

Personally I would toss out those plates if they are cubes.  They look pretty fucky.  There’s nothing wrong with going back to spore and starting over.

jee3apha said:

I'd be interested in your thought process tho, what in this plate made you think it's some kind of contamination?

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it looks like it

disorganized myc. the yellowing? cubes grow like weeds and healthy cultures are easy to come by. they grow like crazy. so anything that looks off? is off.

i look for anything that is symmetrical. thats what i look to when i transfer from questionable areas. does the new growth grow out evenly? if not?? i either take another cut to see if i can get something more uniform or i start over.

its like a relationship. would you rather spend tons of time and energy on something that may not work? or just start over with something fresh?

sometimes tossing out the old can be the speediest way to gaining the new. cant fix em all kinda logic.

mushboy said:

jee3apha said:

I'd be interested in your thought process tho, what in this plate made you think it's some kind of contamination?

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it looks like it

disorganized myc. the yellowing? cubes grow like weeds and healthy cultures are easy to come by. they grow like crazy. so anything that looks off? is off.

i look for anything that is symmetrical. thats what i look to when i transfer from questionable areas. does the new growth grow out evenly? if not?? i either take another cut to see if i can get something more uniform or i start over.

its like a relationship. would you rather spend tons of time and energy on something that may not work? or just start over with something fresh?

sometimes tossing out the old can be the speediest way to gaining the new. cant fix em all kinda logic.

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PuffM4gicDr4gon said:

I've given up on Spore prints before too. Sometimes there's just too much contamination or what is there is just too aggressive

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jee3apha said:

PuffM4gicDr4gon said:

I've given up on Spore prints before too. Sometimes there's just too much contamination or what is there is just too aggressive

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Eh, I'd love to... spore prints are currently my only source. I plan at least to obtain one healthy fruit, then clone it, and resume from there. Hope it makes sense.

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PuffM4gicDr4gon said:

One thing that seemed to make a difference for me is I started using sterile Q-tips to inoculate agar they seem to work better for me than inoculation Loops or anything else.

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Quote:

Your account is old enough for the marketplace if I were you I would just start looking there and try to gather up a few prints until you get one that's easier to work with. Just my opinion though. I totally understand the frustrations of just wanting to get something. I've been there myself

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