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Re: Official Copelandia/Panaeolus Thread [Re: Krs_bmx] * 1
    #29412501 -



Last looks before I sent to bulk. 2 to CV and 1 to horse dung. Giving one of the CV totes away to a local buddy.
I am dehydrating a the two largest pearls right now, along with some grains, Idk if I should do a potency test or try to bury them and see if they fruit after withstanding the dehydration
"Polaris Panaeolopsis" aka Panaeolus cinctulus f. Subsecotioides A2G 9/7, growth over two and a half months was prolific.


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In this life, trust is a luxury we cannot afford.

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Re: Official Copelandia/Panaeolus Thread [Re: Krs_bmx]
    #29412588 -

I have had success with bvi in a tent that was in a room where the temperature was 66f-72f. It was a very inefficient furnace.


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All is one, one is all, everything is.

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Re: Official Copelandia/Panaeolus Thread [Re: trippleblack] * 1
    #29412824 -

I'm still skeptical.  These are bvi and look quite similar to yours.



You mentioned plans to confirm the cross with microscopy?  Are you looking for two spored basidia or have another idea?

I do believe they can cross and plan to try it.  But parasexual breeding is messy and you may have a (bvi*bvi/bispo - partial cross) or a (bvi/bispo*bvi/bispo - full cross) or any number of variations on that depending on how many times nuclei went through a parasexual cycle on the plate.

This all assumes cyan and bispo pheromones like each other.  Which I think they might, my understanding is pseudo homothellic species are pretty horny.

But I do know that aggaricus breeders have struggled a lot to cross bisporus using conventional methods.

I do plan to try it and I'm not trying to neg your project, it's inspiring and I want to try (but using a di-mon so I can verify easily). And I am also skeptical.


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29412886 -

Tree frog you definitely know your stuff.  The cross over rates of parasexual breeding appear to be anywhere between, 25/75 to 75/ 25.  Mycologist do claim it is messy in the literature, but you can guide it in different ways.  I sense Parasexual is very useful in this case because it does not rely on mating type matches like Di Mon.  This parallel close to how agaricus breeders work with protoplast fusion.  I cross with isolations I have intimately worked with and grown for years. 

Yes microscopy of basidia will be one method, also potency testing, and sequencing if needed, if any other ideas let me know. i used to have dreams of finding a three spore basidia :grin:

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Re: Official Copelandia/Panaeolus Thread [Re: trippleblack]
    #29412925 -

Oh I know very little, I just know where to dig :lol:

Cyans can express two spored, but, fortunately, it's very rare in the bvi phenotype as far as I know.

If you scope some two spored, I would be convinced anyway.  Sequencing of course would be better, and there are sequences at least for bisporus coleville.

https://www.inaturalist.org/observations/272104006

And yeah the mating type match is something I am concerned with as I attempt the cross.  Planning to try three different bisporus dikaryons with a blue spring redspore mono, to give them a better chance of success.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29412979 -

Getting poor fruiting in my martha. Pic is Weza.

Spores to agar to rye to hpoo/straw, cased in 50/50. All stages normal on length of time (bout 7 days each step)

I have a two shelf mini martha tent.  3 disc fogger in a 80qt reservoir piped in thru the top. 80mm pc fan on the reservoir. pc fan runs 24/7 on lowest setting. Fogger is on an inkbird at -10 cal, set to 84% (94%) with 7% Rh cooldown. There are some 1/4” holes in the top of the tent plastic. Humidity and temp sensors are hanging an inch below the top shelf (2 shelf tent)

There is a reptile lamp heating a tray of lava rocks and water hooked to a temp controller set at 77f.

Tent is located in a 65f basement, 50% Rh.

this is the best tray out of the tent. Other trays had fruit abort at 1” tall. See second pic TTBVI. Twisting contorting turning upside down shriveling etc would describe them.

I have a jcm tub that works fine in the cold basement but I’d like to figure out this tent.

What to do?

Edited by philmyco (11/26/25 11:53 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: philmyco]
    #29413003 -

Are you misting?  It looks like they're wet more often than they want to be.

If you're not misting, I'd suggest dropping your rH on the low end a few points.  So they have more of a wet/dry cycle.

Evaporation at 86 rH is pretty minimal.  Air flow and light can help but the condensation point is 88 (or even lower, depending on the species).  Meaning, above 88 there isn't much evaporation without assistance.

Also, how often does your humidifier kick over?  Because that will help us have some idea of how long it's staying above 88.  As well as some idea if they are getting enough oxygen.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (11/26/25 12:10 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Krs_bmx]
    #29413128 -

Bump

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Re: Official Copelandia/Panaeolus Thread [Re: Krs_bmx]
    #29413129 -

Krs_bmx said:
Bump


Krs_bmx said:
Hey yall I was wondering if anyone has had success with ttbvi in 70-72°F. I seen that it’s better to have it higher like around 78° but I have no idea how to get that ideal temp since I have no grow tent or anything.any ideas?


Bump

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Re: Official Copelandia/Panaeolus Thread [Re: Krs_bmx]
    #29413130 -

Tf was the point of that


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #29413131 -

Lol oh ok 😂


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #29413150 -

Tryin to get my question answered

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Re: Official Copelandia/Panaeolus Thread [Re: Ratatoskr]
    #29413160 -

Ratatoskr said:
I have had success with bvi in a tent that was in a room where the temperature was 66f-72f. It was a very inefficient furnace.


I've had a similar experience as Ratatoskr, lower than that(63-65) and my plates, jars, and trays will really slow down or stall.

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Re: Official Copelandia/Panaeolus Thread [Re: stagger] * 1
    #29413277 -

Idk why this replied to you stagger, but general question to the forum: Hows this agar plate looking, should I inoculate with this or wait for my 4th transfer to finish? TTBVI



This was the original plate I was working with:



--------------------


“Life moves pretty fast. If you don’t stop and look around once in a while, you could miss it.”

Edited by Miltonius (11/26/25 07:02 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Miltonius]
    #29413350 -

That looks plenty strong to me


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How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29413485 -

tree frog said:
Are you misting?  It looks like they're wet more often than they want to be.

If you're not misting, I'd suggest dropping your rH on the low end a few points.  So they have more of a wet/dry cycle.

Evaporation at 86 rH is pretty minimal.  Air flow and light can help but the condensation point is 88 (or even lower, depending on the species).  Meaning, above 88 there isn't much evaporation without assistance.

Also, how often does your humidifier kick over?  Because that will help us have some idea of how long it's staying above 88.  As well as some idea if they are getting enough oxygen.


The trays that grow fruits need syringe watering once or twice a day. The ones that abort an inch high full tray are always wet.

I dropped Rh to 95-84. Seems like it takes about 25-30 minutes to cycle humidity on but it varies a bit I think based on how much the heater needs to raise temp from humidifier cooling tent a couple degrees. Fogger fan runs 24/7.  No trouble getting pins but all the fruits bruise and caps look weird or the whole tray just aborts an inch tall.

Couple more pics for ya in case it helps.



Edited by philmyco (11/26/25 10:52 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: philmyco]
    #29413507 -

Fogger fan on 24/7 in a small tent.

I didn’t read your other posts, but with that description, sound like you are desiccating your pins.  I’m pretty sure you can’t leave a fan always on when growing pans.  Even at 99% humidity, with a fan always on you will damage the pins and pin set.

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Re: Official Copelandia/Panaeolus Thread [Re: trippleblack]
    #29413749 -

I think I may be drying them out as well. But to devils advocate I guess, dreamer tubs also run a 24/7 positive pressure fan in an even smaller environment than this mini martha.

I’ve been setting up the tent per asuras tek. They use a 4” fogger fan 24/7 in a 4 shelf Tent on almost the lowest setting. I figured a 3” fan on the lowest setting in a 2 shelf would do.

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Re: Official Copelandia/Panaeolus Thread [Re: philmyco] * 5
    #29413792 -

You don't want them always wet.  Especially at high temperature.

I think of it this way.  For pin formation, they want very high humidity.  Because pins can be sensitive and easily dry out.  But for fruit body development, they want high rH to promote condensation.  Quickly followed by periods where that condensation evaporates off.  Generally this is accomplished with an exhaust fan that comes on after the fog cycle, dropping the humidity into the low 80s.

It's the evaporation that keeps the mushrooms cool.  And if the mushrooms are always wet, they can't stay cool like they need.  Leading to poor morphology and possibly aborts.

And keep in mind that heat, air currents, and light all play a role in evaporation in addition to rH.

Anyway, the pictures look like they're staying wet to often during fruiting (but not pinning).  The trick is giving the fruits more evaporation while not aborting the pins.  However you go about it.

And that's just my opinion based on how the photos look and you saying they're always wet.

Edit:  And realize there is a paradox here, the colony senses the warm temperature and fruits at that temperature because that's what gives the spores the best chance of survival.  However, they actually need to keep the fruit bodies cool in order to develop the DNA in the spores as they mature.  And they use evaporation to accomplish that.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29413816 -

The surface conditions are always moist. The fruit bodies get wet when the fogger runs but they evaporate quickly. The top shelf surface conditions are always moist. Bottom shelf surfaces conditions require twice a day misting.  The humidity likes to hover at 87 for long periods of time due to the temperature being raised after fogging. I understand everything you’re saying but not sure what to do.

I’ve increased the cycle to 95->83. Should I add an exhaust fan? Stop the fogger fan? I had this tent on negative pressure and cycle timers before and I couldn’t get it to work at all. Seems to be halfway to working with asuras tek.
Looks like it really struggles to get lower than 86rh…likes to settle and hover at 86-87 forever. If I add exhaust I feel like it will just lower temp in the tent, cause heater to come on, and that will bring Rh back up on its own and the fogger never turns on.

Edited by philmyco (11/27/25 08:49 AM)

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Jump to top Pages: < First | | 1985 | 1986 | 1987 | 1988 | 1989 | 1990 | 1991 | 1992 | 1993 | 1994 | 1995 | 1996 | 1997 | 1998 | 1999 | 2000 | 2001 | 2002 | 2003 | 2004 | 2005 | | Last >

Shop: North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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