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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Asura] * 2
    #28965101 -

Use your readers when doing up-close work? :lol:

If you don't mind them taking up more space, maybe the 5ml tubes would be easier to use with less visual acuity?

My problem is my dominant hand gets shaky sometimes. Often I can attribute it to some repetitive thing I recently did, like pouring too many agar plates in a session, but sometimes it just happens. :frown: Makes it hard to do fiddly work like putting a teeny wedge in a tiny vial, or a bigger wedge into a zipper baggie for mailing.

I have had good results with the inoculation loop method for removing a sample. I make one long enough to reach the bottom of the vial without the handle going into the opening, and I bend the wire where the loop itself starts at about 45 degrees so it can be used as a scoop. Almost any size wedge I've made that will fit in the cryovial will also sit right on top of the loop. Usually it also comes right off when I flip it over a plate, though it sometimes takes a little coaxing.


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LAGM 2026 grow log | My library/trade list

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Phaethon] * 1
    #28965132 -

I’m a big fan of the 5ml vials.  I tried the 2ml but they felt too small.  I gots shaky hands too. 


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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand


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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Psicomb] * 1
    #28965165 -

That was another benefit of the vial rack I printed that hadn’t occurred to me. I made it for storage but it was such a help when transferring to the vials. Lined a bunch up in a row with the lids loose and was able to quickly and easily transfer little chunks in without having to worry about hanging on to tiny little vials with my big ole fumbly hands.

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Phaethon] * 2
    #28965177 -

Phaethon said:
Use your readers when doing up-close work? :lol:



LOL I need readers on top of my readers. Yeah man, 5ml vials sounds way better to me.
I get the shaky hands too....my god I should post a pic of my writing on these vials.


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Cultivating Panaeolus cyanescens


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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Mycoplex] * 1
    #28965333 -

Thank you so much for sharing this tek.You literally saved my life.
I just wish I had found this thread earlier so I would not have so little fridge space because of all my culture slants.
Could I ask some questions about the tek? Is it okay if the tiny piece of mycelium I put into the vail doesn't have much agar attached?
And because the mycelium itself was so lightweight, it kept floating on the surface even after I tried to move it down by agitating the vails several times. Is it okay if the mycelium stays on the surface of water?
I am very new to this forum and unfamiliar with it. Hope I can successfully post the pictures for reference.


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Learn, Learn, and Learn!

Edited by Show Chiang (09/28/24 04:30 PM)

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Show Chiang]
    #28965604 -

Show Chiang said:
Thank you so much for sharing this tek.You literally saved my life.
I just wish I had found this thread earlier so I would not have so little fridge space because of all my culture slants.
Could I ask some questions about the tek? Is it okay if the tiny piece of mycelium I put into the vail doesn't have much agar attached?
And because the mycelium itself was so lightweight, it kept floating on the surface even after I tried to move it down by agitating the vails several times. Is it okay if the mycelium stays on the surface of water?
I am very new to this forum and unfamiliar with it. Hope I can successfully post the pictures for reference.



Welcome to the thread and welcome to Shroomery!  Your vials and samples look good to me.  The amount of agar should only factor in how easy or hard it is to retrieve the sample.  Your work looks clean to me, and the fact that the samples float should also be OK.  In the future you might want to include slightly more agar just to give the sample more substance and weight, but otherwise I would consider those vials done correctly.


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Cryovial Tek
Indoor Ovoid Community
ATL7 Cosmoplex Gallery
Fungus Gnat Control Tek
Precise Pasteurization Tek

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Mycoplex] * 1
    #28965704 -

Got it! Thank you so much for your response.💕🥹 I plan to slowly pass my entire library from slants to this form for preservation purposes. It’s going to be a huge workload.😬😬😬


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Learn, Learn, and Learn!

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Show Chiang] * 1
    #28968669 -

Last week I've revived an Oyster culture that's been in this storage for 1.5 years. Seems plenty strong so far. I'll try to fruit it later. :rockon:


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Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Adas] * 2
    #28968799 -

Did 20 x 5ml for storage and 5 x 2ml for shipping last night. 5 ml is certainly easier to work with, but I still like the 2 ml for shipping.

I include bigger pieces and more agar in mine, so it probably takes longer to go into actual stasis, but they have always recovered easily. I often pour straight to grains with great success. Not to imply that this is best practice, but it has worked out well for me.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Adas] * 2
    #28970213 -

Adas said:
Last week I've revived an Oyster culture that's been in this storage for 1.5 years. Seems plenty strong so far. I'll try to fruit it later. :rockon:


Sounds awesome! I will try to revive mine after one year and see how things go. Hope they work fine. :smile:


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Learn, Learn, and Learn!

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: ReverendMyc] * 1
    #28970224 -

ReverendMyc said:
Did 20 x 5ml for storage and 5 x 2ml for shipping last night. 5 ml is certainly easier to work with, but I still like the 2 ml for shipping.

I include bigger pieces and more agar in mine, so it probably takes longer to go into actual stasis, but they have always recovered easily. I often pour straight to grains with great success. Not to imply that this is best practice, but it has worked out well for me.


I’m curious about how to tell if mycelium enters the state of dormancy. :confused:


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Learn, Learn, and Learn!

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Show Chiang] * 1
    #28970236 -

I am not sure that it is clearly defined. Mine continues to grow a bit of fuzzy mycelium on the little wedges for a while, but eventually it stops. Then when I use it later it kicks right off again on agar or grain. Sometimes takes a couple of days more than if it was a fresh plate transfer, so I reckon those were in full stasis. But I don't know how you would know that for sure without testing them on recovery plates.

But if it works, I don't sweat those details.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: ReverendMyc] * 1
    #28971764 -

I just checked the culture vials I made last Saturday and found that most grew a bit fuzzy mycelium from the edge, except for the one I attached no agar with. This poor little guy must have run out of nutrients to grow anything. I almost feel bad for it.:sad:


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Learn, Learn, and Learn!

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Show Chiang]
    #28980010 -

Hey guys I added a "Community Work" section to the front page post where I linked many of the pictures posted in the thread here by others.  If I missed any I will take another look and add them in.


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Cryovial Tek
Indoor Ovoid Community
ATL7 Cosmoplex Gallery
Fungus Gnat Control Tek
Precise Pasteurization Tek

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Mycoplex] * 1
    #28980149 -

Oh, almost forgot. Couldn’t stand my sloppy handwriting on the tiny little vials so I picked up a cheap label maker. Much easier that trying to scribble on them.


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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Henry Swanson] * 1
    #28980188 -

What are you using and how well does it stick?

I tried a roll-based label maker (plastic or at least laminated thermal tape), and the edges of the label are always coming off, though I haven't had one actually fall off yet. I also had to trim them, which was annoying. I went back to writing with the teeny Sharpie. :lol:


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LAGM 2026 grow log | My library/trade list

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Phaethon] * 1
    #28980196 -

Phaethon said:
What are you using and how well does it stick?

I tried a roll-based label maker (plastic or at least laminated thermal tape), and the edges of the label are always coming off, though I haven't had one actually fall off yet. I also had to trim them, which was annoying. I went back to writing with the teeny Sharpie. :lol:


I used this, I did trim them a little but it would have fit without doing so. Also picked up some clear labels for my agar plates. But yeah it sticks really well.

Phomemo D30

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Henry Swanson] * 2
    #28985628 -

The product you use is on sale now and I think I'm going to order one. Writing down all the information on the tiny cap is killing me😩



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Learn, Learn, and Learn!

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Re: 'Cryovial Tek': Long-Term Storage of Cultures with Cryogenic Vials [Re: Show Chiang] * 3
    #28987527 -

Nice work guys!  Here is a photo I took of the revived 2-year old culture that I fruited.  The uniformity of the clone still came through like expected after revival.  It's a cubensis 'Tasmanian' culture I isolated in 2022.



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Cryovial Tek
Indoor Ovoid Community
ATL7 Cosmoplex Gallery
Fungus Gnat Control Tek
Precise Pasteurization Tek

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Re: Tek: Long-Term Storage of Cultures with Cryogenic Vials (Cryovials) [Re: Mycoplex] * 1
    #28987540 -

This is super cool, definitely saving this.


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APE x NSS cross I created


Cluster heaven.

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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Mono Tub Substrate   North Spore Injection Grain Bag

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