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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: Mr Piggy]
    #28765379 -

Designed specifically to handle a wide range of chemicals, high temperature, etc.

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: Mr Piggy] * 4
    #28765380 -



Barbed wire inoculation loop for the kitchen sink challenge

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: Stipe-n Cap]
    #28765411 -

Geeez! I see the need for DIYing it :sadyes:

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: LogicaL Chaos] * 1
    #28765415 -


Clone snipper stabber scissors.


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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: Stipe-n Cap]
    #28765496 -

Stipe-n Cap said:
Designed specifically to handle a wide range of chemicals, high temperature, etc.



As is a PP5 yogurt container.  I could see a device like that being $40, but ten times that?

I dunno, it's just baffling.  Not knocking you, just trying to get my head around the concept.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Post Your New Tools Of The Trade. [Re: Guerrilla] * 1
    #28765525 -

Guerrilla said:
BeefSupremeJr said:


yes, i ordered 20 more hooks to hang all my plates. i really only keep around 24 plates these days so there will be 4 rows of six.  cheap chinese sombitches were 17$ for 20 of them.  some mounting tape.  a level.  this will feel really cool when im selecting plates to send.  this will also help me to not horde.  ive had upwards of 200 plates at a time a its bad for my mental health.





Not been around in a minute so I've only just seen this - slick rick shit Beef.

Not only does it look good, but totally purposeful as I get the hoarding thing too.




oh my god im happy to see you

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: Mr Piggy] * 3
    #28765541 -

Mr Piggy said:
Stipe-n Cap said:
Designed specifically to handle a wide range of chemicals, high temperature, etc.



As is a PP5 yogurt container.  I could see a device like that being $40, but ten times that?

I dunno, it's just baffling.  Not knocking you, just trying to get my head around the concept.



It is the fancy name (bioreactor) that catches the hefty price tag.  :lol:

It is cool to see you guys using these things.  Before I took a break, I was getting into figuring out things like bulk substrate and grain sterilized together in spawn bags and noccing them with LC.  Also, I was on the brink of building one of these bioreactor-type thingies for my inoculation gun. 

Now that I am back in the fold, as go around the curve of picking the hobby back up and getting everything I need that I did not hang onto, I am planning on picking back up in the experimental techniques and exotic mushroom department where I left off.  It is cool to see you younger guys have taken it past where I left off.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: hamloaf]
    #28765580 -

How do bio reactors work without laminar flow stipe?

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: Stipe-n Cap]
    #28765607 -

Is the reactor used to supercharge the LC growth, like it does when they are growing tissue culture plants in the multiplication stages? I thought the air was mostly for pressurizing the containers enough to flood and drain the solution up and down into the explants, just like an ebb & flow, but not sure how it would work with LC that is always submerged anyway?.. Does it just pump filtered air down through the tube and aerate the broth like deep water culture hydroponics? Will you be connecting multiple bottles together? Is it only used on some kinds of mushrooms cultures?  Either way it's rad, and hope you do some kind of tek\writeup on reactors!

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: PsillySeeEms] * 1
    #28765617 -

Just when I feel like I’m grasping the beginning of something Stipe comes around with a bioreactor — also seen a killer induction coil build recently.

Y’all are ON IT! Inspirational.


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Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: Mr Piggy] * 2
    #28765698 -

Mr Piggy said:
Stipe-n Cap said:
Designed specifically to handle a wide range of chemicals, high temperature, etc.



As is a PP5 yogurt container.  I could see a device like that being $40, but ten times that?

I dunno, it's just baffling.  Not knocking you, just trying to get my head around the concept.



Its the same reason why a consumer grade bolt used for a fighter jet will cost $3K. When selling to large financially/government backed companies like a laboratory you can charge whatever you want because nobody audits that shit and the high cost is never questioned

It just goes down the chain justified by each person taking a piece of the slice

Edited by CocaineBuffet (05/07/24 10:04 PM)

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: CocaineBuffet] * 6
    #28765740 -

One of my pride and joys, she gets me through those long nights of lonely sterilization work. 30qts of pure happiness. :sun::mushroom2::rocket:


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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: CocaineBuffet] * 2
    #28766054 -

CocaineBuffet said:
Mr Piggy said:
Stipe-n Cap said:
Designed specifically to handle a wide range of chemicals, high temperature, etc.



As is a PP5 yogurt container.  I could see a device like that being $40, but ten times that?

I dunno, it's just baffling.  Not knocking you, just trying to get my head around the concept.



Its the same reason why a consumer grade bolt used for a fighter jet will cost $3K. When selling to large financially/government backed companies like a laboratory you can charge whatever you want because nobody audits that shit and the high cost is never questioned

It just goes down the chain justified by each person taking a piece of the slice



There is also the matter of economies of scale. In some applications, failure isn't really an option and everything needs to be manufactured to very specific parameters. If a company or facility is devoted to producing only one product, but only sells a few thousand units a year, then the final unit price will reflect that.

For something like fighter jets, there's little incentive to bring costs down, as bloated military budgets are the only feasible income source. And as an aerospace contractor, you're just in a big ole circle-jerk with your friends in DoD anyhow.

For something like bioreactors, they should get cheaper in time as more of them are produced, manufacturing is streamlined, and engineers figure out where corners can be cut.


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ACAB  🖕🧊


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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: B Traven] * 2
    #28766069 -

Has anyone looked into these and or tried them yet? I believe its a similar lid to some of these "Bioreactors" everyone is talking about currently but its like a 15 dollar lid.

https://browntreasure.com/products/myco-lid-pro%E2%84%A2


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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: RichMyco] * 6
    #28766133 -

That is pretty cool.  Even though I am positive that I could construct something better, a prefabricated deal would save the time of having to source and gather all the materials needed to build one.  I may pick one of those up.  Would have to modify it still, but only slightly. 

Do not let the word “bioreactor” intimidate you.  A literal bioreactor’s purpose is to handle all kinds of different organisms and such.  In our hobby, we are only dealing with one organism, so a simple mason jar with lids modified in bioreactor fashion will suffice just fine.  It does feel fancy to refer to it as a bioreactor though.  :lol:


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: RichMyco]
    #28766181 -

RichMyco said:
One of my pride and joys, she gets me through those long nights of lonely sterilization work. 30qts of pure happiness. :sun::mushroom2::rocket:





Have looking for I for this type of cooker for mushrooms Magic have been of checking in the store of Thrift and no Luck yet, I am happy to have oportunity to look for these of items for the grows! thanks of You for sharing of this


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blessings and love Friends as I am Learn new Language English, I am Vighnavinashanaya I come from India and in America here now I adventures do make with mushrooms magic, Friends of Love in hearts, Namaste and forever blessings:heart::sun::mushroom2:

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: hamloaf] * 1
    #28767363 -

Okay, so, what does the bioreactor do?

:lol::shrug:


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: Mr Piggy] * 1
    #28767390 -



I wonder if you could setup a DIY temporary immersion bio reactor system like this ^, and fruit mushrooms in the containers instead of using for plant tissue cultures.. Use some kind of screen like a netbasket or mesh sheet for the mycelium to take hold on?  Grow full sheets of potent mini plins, or huge chonkers? Keep the grow in vitro the whole way, without any contam or messing with substrates\mediums\field capacity\etc, and exposing anything to open air until harvest.

I guess you could test with a simple bio coupler first, that attaches 2 vessels together(and allows gas exchange), and be able to shake it up or turn it over by hand every so often without all the automation and pumps..

Mycoponics?! :shocked:

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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: PsillySeeEms] * 10
    #28767730 -

This is the thingies. It came from midwestand it tests to see how many particles are in the air, below .3 microns, etc
I bought it to see if the HEPA was actually working, and to see the limit of how far away it worked, etc.
Well, it taught me some shit about a few things, like...
When you turn the ffu on, it takes a while for it to be totally free of contaminants, like 2 min...
That if you leave the ffu on all night, there is a large are with no contamination.
If you open the door to the room with the ffu on, contaminants go in.


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Re: Post Your New Tools Of The Trade 🧪⚫🔬 [Re: fnulnu] * 5
    #28767911 -


Edited by Yahra (05/09/24 03:21 PM)

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Shop: North Spore Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale


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