This Mushroom Cultivation is for magic mushrooms, Gourmet and Medicinal Mushrooms is for, well, those. I think there is general consensus that LM is the latter, but one could start a philosophical discussion about that. There is also another forum space for that too haha. Anyways... Let admin decide about where to store. But there is some overlap regarding your mycelium question with this forum.

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Am I right in thinking these plates are done-for, or is there a reason to try and recover them? It was potato-starch agar, if it matters.

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I searched on the forums and it seems like wood splints (basically a piece of wood in agar?) are the best way to store good clones long term. Or is there a better way?

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Apparently the big benefits are in the mycelium rather than the fruit body for Lion Mane specifically. Am I right in thinking the best way to bulk-grow mycelium is to look into liquid culture methods? If I wanted to yield 2.5g of mycelium per week, would I need 1 quart jar of LC? 2? 20? Some idea of scale is all I'm really after.

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>>I have the feeling it is also just very accidental and very convenient for supplement producers that instead of just only the fruiting bodies, also the mycelium is usable.

Anyways, what I understand is that there are more variables that make some contents (more) active. It is not just 'mycelium OR fruiting bodies', but more variables play a role. Just like the nutrients content label on a package for potatoes makes one conclude the nutritional value is always identical (regardless of time of the year, temp and sun conditions, soil quality, variety, NPK, etc.).

For actives for example (from magic mushrooms) it seems alkaloids seem to be more activated in mycelium when there is a threat, like mites. The same principle of influence of context conditions quite probably apply for lots of contents.

So, if you are talking about 'the best way'; do you mean BE or ABE? Like yield in grams or yields in grams of the specific chemicals you're looking for? Based on that, and practical possibilities, different answers about the best way.

I'm not familiar with extracting mycelium from LC. I think I've read some people do that for magic mushrooms. If there is a tek to extract mycelium from LC for magics and other mushrooms, I am keen to know. Distill the liquid and store the salt? Regarding bulk grow and yield, my guess is that for the same size jar of LC vs grain, grain jar will have more yield of mycelium. And additionally you have grains in your yield. One can then also state it has additional β-Glucans! Actually yesterday I dried LM substrate (with rye berries). For sure it is visibly less dense colonized then compared with cubes.

cybork said:

Some reading possibly of interest:

Cryovial tek by Mycoplex
What is the rationale behind adding distilled water to a piece of agar (...) for long term storage? by me
The OFFICIAL Stasis Storage Thread by fossilshark
homemade lids for drying out cultures for storage by fahtster

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Thanks. I'll check those out.

cybork said:

>>I have the feeling it is also just very accidental and very convenient for supplement producers that instead of just only the fruiting bodies, also the mycelium is usable.

...snip...

So, if you are talking about 'the best way'; do you mean BE or ABE? Like yield in grams or yields in grams of the specific chemicals you're looking for? Based on that, and practical possibilities, different answers about the best way.

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I genuinely have no idea what BE and ABE means. I checked the glossary, and asked google.

As for it being 'very accidental' that fact is not lost on me. There's a reason I'd rather grow my own than buy from him. He gets nothing if it doesn't work, and if it does, then I gain a little bit of faith in humanity.

When I talk about yield, I mean that the schedule for dosing is 5 days on, 3 days off. So within a 7 day period (because buffer) you need to be able to grow at least 2.5g of edible mycelium. That's the metric. I...see it no different than making cookies. You need X amount of butter. Either it grows that fast or it doesn't. And, sure, there's variability. Maybe my honey is different from yours. Maybe my water says it is distilled and it isn't. So maybe I end up needing to crank it up to get what I'm after. But if a quart jar grows so fast that I'd be looking at 50g a week from a quart jar, well, ok then.

Also, to clear up any confusion, you eat the fruiting bodies of the cubes, but the mycelium of the LM. And, yeah, I agree, convenient he happens to sell that. But I'm not buying from him, because I prefer to verify science when I can. I can buy lion's mane from the local farmer's market and get my myc going from there.

A.k.a said:

If the plates are still clean you can take a bit of the dried up agar and put it on a new plate.

I recently did this with a plate that was a couple years old and looked like a potato chip, came back to life in 2 days.

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