Stipe-n Cap said:

[...]
Soaking fully colonized grain spawn before spawning;
[...]

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Wait is this debunked? I got my info from Frank: https://www.shroomery.org/forums/showflat.php/Number/17616982

Yeah it's 10 years old but I thought it was still valid

Throwaway said:

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Throwaway said:

Stipe-n Cap said:

[...]
Soaking fully colonized grain spawn before spawning;
[...]

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Wait is this debunked? I got my info from Frank: https://www.shroomery.org/forums/showflat.php/Number/17616982

Yeah it's 10 years old but I thought it was still valid

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Stipe-n Cap said:

Throwaway said:

Stipe-n Cap said:

[...]
Soaking fully colonized grain spawn before spawning;
[...]

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Wait is this debunked? I got my info from Frank: https://www.shroomery.org/forums/showflat.php/Number/17616982

Yeah it's 10 years old but I thought it was still valid

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Yeah, it's pointless.

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perro said:

Cold shocking

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Stipe-n Cap said:

Yeah, correk on all points.  I think it was very popular in it's day. I don't think people applied as much critical thought to methodology as we do now. It's been interesting to watch the evolution of cult over the years, this site and the hard working, clever, citizen scientist types, have really developed a wide variety of accessible, reliable techniques for the cultivation of so many diverse species.

It really is something, when you think of it. Mushrooms used to be soley in the hands of only a very select few, now it's open to everyone, and tailored to nearly every skill level and preference.

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Quote:

The sterilization/decontamination activity of UV lights is limited by a number of factors, including:

Penetration – In the dynamic air streams of BSCs, microorganisms beneath dust particles, plastics, and work surfaces are not affected by the UV light because it cannot penetrate particles so far from the UV source.

Relative humidity – The germicidal effects of UV light drop off precipitously when relative humidity is above 70%.
Temperature and air movement – The optimum temperature for the UV lamp to be effective is 77-80 degrees F. Temperatures below this range result in reduced efficacy, and air movement can exacerbate this.

Cleanliness – Dust and dirt block the germicidal effectiveness of the UV lamp, so weekly cleanings are necessary.

Age – Check UV lamps every six months to assure proper function, as the amount of germicidal wavelength emitted decreases with bulb age and hours of use.

Overuse – UV lights are routinely left on overnight or longer in an effort to decontaminate workspaces, but this practice can result in the germicidal wavelength no longer being produced by the bulb.
For these reasons and other concerns, the National Sanitation Foundation (NSF) does not recommend the use of UV lights in BSCs.

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Mainet2r12 said:

🔥 yeah!  Call me Sam Macgee …

“ The water in your tub is tap water, it's not sourced from a sewage system, it's fine. UV lights are beneficial when used in well systems in line and potentially coupled with a filter, but that's just common SOP for rural communities”

We have well water…

It has not made is sick, ever, but it is not treated. And it is a shallow well (25’ or so).

The uv light is freaking huge and it gets placed directly over the water, not off to the side. Yes, almost all UV-C is blocked by glass. No, invisible airborne water does not significantly block UV-C. Yes, it does not pass through opaque material. Yes, it does kill by breaking up DNA, but is not full effective in breaking down DNA in bacterial endospores (thanks for that, it made interesting study).

As for 70% iso being effective, it can be, but…what Ive read in multiple sources is that it must remain on the surface being cleaned for 15 minutes minimum, which is why 70%; anything higher would evaporate too quickly to complete the cleaning.

Sources suggest that 10% bleach is more effective…

Where contamination is concerned I’d venture that there is no such thing as too much caution; given a grower’s willingness to expend the effort and funds.

Sometimes I ponder the very existence of Psilocybe cubensis in the natural world. How, without our diligent and careful assistance do these poor fungi survive to spore?

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