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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Ashtray161]
    #27302708 -

Nice. I haven't nearly enough deets to say if you have a snowball's chance in hell at being successful but you seem intelligent enough to pull this off. Interesting choice with the sauna. How hot did it get in there?


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: verum subsequentis]
    #27302721 -

sauna would be such a rad room to set up the SAB in, dang. I wouldn't bother with heating it first, though, that will just create air currents from the convection created by the heater. still air box in a room with completely still air is :thumbup:

a tip for you, since I can tell you are very resourceful in digesting teks and methods - in the advanced search, specify that you'd only like to see threads newer than 5 years old. There have been so many advances in recent years that your brainpower would be better spent looking at those. The peroxide stuff all sounds like the kind of stuff that used to be done before more failsafe methods were proven out in recent years. It can be very alluring when you're new to the hobby, but as you dive further in you'll look back and realize all the ways going down that path would have made things much more difficult for you in the long run.


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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: verum subsequentis]
    #27302726 -

Looks good , I use the unmodified one and just slide it off the table a bit .

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Samushlove]
    #27302754 -

Cool to see you've made the effort to figure this all out.

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: DERRAYLD]
    #27303317 -

Ok I have the plates at a constant temp of 80 degrees. Anything else I need to do?

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Rakali]
    #27303327 -

Also anyone in Australia that could suggest a brand and source for WBS. I'm new here. Thanks

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: verum subsequentis]
    #27303339 -

It currently gets to around 160f. It's actually broken and I'm heating it with a space heater which I removed before I did the SAB work. Figured it couldn't hurt to heat it before I started. I know 160 isn't quite enough to kill most things but perhaps it killed some. Also the temp had cooled down before I got in to work.

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: trubblesome]
    #27303347 -

Thanks for the tip!

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Rakali]
    #27303352 -

Start putting some spores and mush tissue center of plates contamination usually pops up on the on edges , wait 24  48 hrs

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Samushlove]
    #27303660 -



Spores printed! Only I left them on for way too long. I thought I read that they needed to be there for 24-48hrs. I don't know how I got that idea but it's way wrong. Anyway it's done. I'm drying them in the sauna at 80f. Would you recommend scraping a bit off and putting it on the agar now? I'll need to reheat the extra agar and go buy some ketchup cups. I'm guessing you'll say yes so I guess that's what I'm doing. Also while I'm out I'll buy birdseed. I found 5kg pretty cheap. It does have corn but it seems worth it. Would it make sense to use birdseed for my spawn jars as well as my fruiting chamber? I'm going to start fruiting in 10L buckets to start.

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Rakali]
    #27304465 -

Yeah put spore's to agar , and yes to the bird seed I get the cheap One with Millet being the main grain , 12 hour soak and a good rinse put in jars and PC 90minz there's a hundred teks but wbs is what I'm using as of now ,I only have a few grows under my belt.

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Samushlove]
    #27304570 -

I haven't inverted the plates yet because I'm worried the pieces of stipe won't stick to the agar. How long should I wait? It's been about two days.

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Rakali]
    #27304607 -

In my experience 2 days and you should see growth , should be good to invert but I don't see the need to.

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Samushlove]
    #27304731 -

There is no need to invert. Keep them in a temp stable area and you're good.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: verum subsequentis]
    #27304936 -

Ok there's definitely a bit of white fuzzy edge on some of the stipes in the agar. Not seeing any contam yet. I did invert them because there's a bunch of condensation in them.

I'm going to make foil envelopes for the spore prints and possibly hold off on putting spores on plates because I have all these stipe plates going right now.

Going to soak my WBS overnight and rinse and PC and make some grain jars and let them sit a week or two just to ensure they are sterile.

So far so good.

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Rakali]
    #27310642 -

I made some WBS following @mushboy's "24hour soak no cook" tek. Only mods were I didn't skim (there weren't many floaters) and I actually ended up soaking more than 24hrs accidentally. Because I can't find my big PC pot I instead laid my jar on it's side with the lid tightened and propped on a jar lid. Seemed to work fine.



One of the plates looks pretty promising. Nice fuzzy growth coming from the center. Do I need to wait for it to grow more or can I just add it to the WBS? It looks free from contams as far as I can tell. Can't say that for some of the others.


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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Rakali]
    #27310713 -

I have never seen such quick learning lol

Good work. I hope to see how this turns out and if you get some fruits!

Hats off sir.

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Salvitica]
    #27317947 -

Because I'm using these stupid little connecting containers I can't get a good luck at the mycelium growth. Only one plate looks promising. It grew white fuzz (not fluff like bread mold but doesn't appear to be branchy either from the angle I'm looking at it) from the center right out to the edges. But it's hard to see if it's mycelium or contam. How can I tell one from the other Is contam usually not white? I have this WBS spawn jar ready to go but I don't want to waste it on some random white mold. Any help would be appreciated. None of the other plates look good so I made go to plan b (spores) sooner than later if this one plate turns out to be contam.

Also just for fun I added a bit of moisture to that dirt/manure/mycelium sample bag I collected and broke it up a bit. Probably could have done that sooner. It doesn't cost me anything to give it the right conditions and see if it will colonize. If it does I'll treat it like a spawn bag and try to fruit it.

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Rakali]
    #27387847 -

My mushroom project has been on hold for awhile (53 days according to the old post warning). In the meantime the WBS spawn jar I made about 2 months ago got moldy in the last week. It was pretty good for awhile and had I used it sooner it probably would have been fine. I think the cap wasn't fully sealed and I hadn't taped it.

Of all the 12 agar plates I made only one looked uncontaminated (creamy white). I had been intending to spawn it but I kept putting it off. I looked at it this morning and there's a mushroom growing in the plate!



This is encouraging me to spawn that plate so I'm going to make another WBS jar and spawn it in the next few days.

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Re: Overexcited Noob with 85g of Fresh Cubes and an Interest in Cultivating Seeking Advice [Re: Rakali]
    #27388228 -

Rakali said:
My mushroom project has been on hold for awhile (53 days according to the old post warning). In the meantime the WBS spawn jar I made about 2 months ago got moldy in the last week. It was pretty good for awhile and had I used it sooner it probably would have been fine. I think the cap wasn't fully sealed and I hadn't taped it.

Of all the 12 agar plates I made only one looked uncontaminated (creamy white). I had been intending to spawn it but I kept putting it off. I looked at it this morning and there's a mushroom growing in the plate!



This is encouraging me to spawn that plate so I'm going to make another WBS jar and spawn it in the next few days.


Clone that plate pin and through that plate on grain:thumbup:

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Shop: Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Bulk Substrate

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