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OfflineAkrobatik
Chillin

Registered: 01/16/04
Posts: 267
Last seen: 10 years, 4 months
Pan cyans, on PDA?
    #2635368 - 05/04/04 02:45 AM (13 years, 2 months ago)

Has anyone had any problem cultivating pan cyans using Potato Dextrose Agar? Seems like the mycelium doesnt like the PDA. Would MEA work better, or is there another Agar that would be best suited for the job. Thanks ahead for any insight.


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:mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


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InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Pan cyans, on PDA? [Re: Akrobatik]
    #2635391 - 05/04/04 02:49 AM (13 years, 2 months ago)

I dont do agar work on my Pans


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So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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OfflineAkrobatik
Chillin

Registered: 01/16/04
Posts: 267
Last seen: 10 years, 4 months
Re: Pan cyans, on PDA? [Re: OldSpice]
    #2635399 - 05/04/04 02:52 AM (13 years, 2 months ago)

Can I ask Why?


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:mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


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InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Pan cyans, on PDA? [Re: Akrobatik]
    #2635411 - 05/04/04 02:55 AM (13 years, 2 months ago)

I use multispore injection...i just grow them and dont try and isolate anything


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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OfflineRoseM
Devil's Advocate
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Registered: 09/24/03
Posts: 22,294
Loc: Mod not God Flag
Last seen: 10 days, 5 hours
Trusted Cultivator
Re: Pan cyans, on PDA? [Re: OldSpice]
    #2635418 - 05/04/04 02:56 AM (13 years, 2 months ago)

Al's lazy... so am I.


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Fiddlesticks.



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OfflineAkrobatik
Chillin

Registered: 01/16/04
Posts: 267
Last seen: 10 years, 4 months
Re: Pan cyans, on PDA? [Re: OldSpice]
    #2635423 - 05/04/04 02:57 AM (13 years, 2 months ago)

Wouldnt it be better to try and isolate a fruiting strain, especially with a somewhat picky strain to grow indoors, that way you can at least get some consistancy.


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:mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


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Anonymous

Re: Pan cyans, on PDA? [Re: Rose]
    #2635428 - 05/04/04 02:57 AM (13 years, 2 months ago)

hehe, well akro and I are trying hard to get one to isolate...  PDA is not working... :frown:  I can't figure out what to use... maybe PDYA?


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OfflineAkrobatik
Chillin

Registered: 01/16/04
Posts: 267
Last seen: 10 years, 4 months
Re: Pan cyans, on PDA? [Re: ]
    #2635429 - 05/04/04 02:58 AM (13 years, 2 months ago)

OR MEA


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:mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


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OfflineRoseM
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Posts: 22,294
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Re: Pan cyans, on PDA? [Re: Akrobatik]
    #2635431 - 05/04/04 02:59 AM (13 years, 2 months ago)

Sure it would... but if you caqn't get it to grow on agar, perhaps you will have better luck on substrate.

Perhaps you have a dud syringe.

Chances are, like cubes, you will get a fruiting strain.


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Fiddlesticks.



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OfflineAkrobatik
Chillin

Registered: 01/16/04
Posts: 267
Last seen: 10 years, 4 months
Re: Pan cyans, on PDA? [Re: Rose]
    #2635435 - 05/04/04 02:59 AM (13 years, 2 months ago)

I doubt its the syringe, came from a reputable place


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:mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


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InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Pan cyans, on PDA? [Re: Akrobatik]
    #2635436 - 05/04/04 02:59 AM (13 years, 2 months ago)

No not really because this strain ieFlorida has been consistant from a wild print...it is pretty hardy allready on its own


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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OfflineAkrobatik
Chillin

Registered: 01/16/04
Posts: 267
Last seen: 10 years, 4 months
Re: Pan cyans, on PDA? [Re: Akrobatik]
    #2635438 - 05/04/04 03:00 AM (13 years, 2 months ago)

I think ralphsters


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:mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


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Anonymous

Re: Pan cyans, on PDA? [Re: OldSpice]
    #2635497 - 05/04/04 03:16 AM (13 years, 2 months ago)

its from ralphster44.. I dont' think its the spores.. I think I am just feeding the spores the wrong food.(in lamens terms)

niether of my pans are growing on PDA agar...I can't remember were I got my pan subb print.. It was a wild print though.

Cervantes - I agree with you, I could just do a multispore. But I want more than that... I want to start from agar to increase my gemination rate, plus to pick out a healthy growth.


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InvisibleOldSpice
Geritol Breath...
Female User Gallery

Registered: 08/25/03
Posts: 59,080
Loc: Crankytown, Texas
Re: Pan cyans, on PDA? [Re: ]
    #2635526 - 05/04/04 03:27 AM (13 years, 2 months ago)

Remember me when you get this Copelandis superstrain going...


--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty


You never see a motorcycle parked outside a Psychiatrist office:biker:


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Anonymous

Re: Pan cyans, on PDA? [Re: OldSpice]
    #2635559 - 05/04/04 03:39 AM (13 years, 2 months ago)

I will surely hit you up some. :cool:


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Pan cyans, on PDA? [Re: ]
    #2635617 - 05/04/04 04:17 AM (13 years, 2 months ago)

I've had some luck using instant mashed potatoes .
And, you might ask bluemeanie if he will give up his recipe .


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Invisible@cro
new name
 User Gallery

Registered: 12/07/02
Posts: 1,224
Loc: The PNW
Re: Pan cyans, on PDA? [Re: ]
    #2635682 - 05/04/04 04:38 AM (13 years, 2 months ago)

Grow them out multispore then take a clone of what fruits...
It seems like you're working backwards trying to isolate something, then fruit it and see how it performs. Go the other way and fruit it multispore, then you'll know the clone you pickt is an easy fruiter and a fast grower.

Peace - @cro


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Pan cyans, on PDA? [Re: @cro]
    #2635717 - 05/04/04 04:51 AM (13 years, 2 months ago)

agreed :thumbup:


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OfflineRoseM
Devil's Advocate
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Registered: 09/24/03
Posts: 22,294
Loc: Mod not God Flag
Last seen: 10 days, 5 hours
Trusted Cultivator
Re: Pan cyans, on PDA? [Re: Akrobatik]
    #2636327 - 05/04/04 11:12 AM (13 years, 2 months ago)

Weird that PDA doesn't work, has this batch worked with anything else?


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Fiddlesticks.



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Anonymous

Re: Pan cyans, on PDA? [Re: Rose]
    #2636576 - 05/04/04 01:34 PM (13 years, 2 months ago)

yeah, my cubes are loving that PDA agar. I am getting great growth on all of them. its just seem my pans are not liking it.

@cro - My pan subbs need agar work done. they are a wild print. I agree with you though, its might be easier to multispore then clone. I just want to isolate then clone from that... maybe my reasoning is off.


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