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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Please advise on result of spore inoculation
    #26100383 -

I have started over with spores on agar of different varieties. Have used the swiping method of zigzag in 3 different sectors on the agar dish and marked the sectors accordingly.
Please comment on the pictures, if these look like normal spore inoculation results or if there is obvious mold. I am unsure, what could be mold compared to mycelium.

1 (nothing growing here it seems):


2 (dont know, could be mold to my eye or tomentose growth?):


3 (looks like best section to transfer from at the intersection between 2 and 3):


4 (again dont really know, any mold here or is it mycelium?):


5 (some rhizomorphic growth in the overlapping sections 1 and 2, transfer this?):

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Re: Please advise on result of spore inoculation [Re: Dr.Ivan]
    #26100391 -

I would transfer it's hard to tell honestly you can make the next agar lower in nutes and see what it looks like after the transfer


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Re: Please advise on result of spore inoculation [Re: Boogieman47]
    #26101289 -

Boogieman47 said:
I would transfer it's hard to tell honestly you can make the next agar lower in nutes and see what it looks like after the transfer


Would you prefer to transfer from the 3rd section generally or does this not matter, if there is no obvious contam visible?

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Re: Please advise on result of spore inoculation [Re: Dr.Ivan]
    #26101380 -

You want to usually stay away from the edge of the plate .. preferably you want to take transfers closer to the center then the edge try to take atleast 2 transfers from each of those plates


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Re: Please advise on result of spore inoculation [Re: Boogieman47]
    #26101401 -

Ok, will do and keep development posted here

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Re: Please advise on result of spore inoculation [Re: Dr.Ivan]
    #26101404 -

Right on good luck we will be here when you need it


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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