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Offlinetotal
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Re: Mephisto's Master Slant Tek [Re: Mephistophelian]
    #15792553 - 02/11/12 12:46 PM (12 years, 7 days ago)

:fuckinawesome:

And also Mephistophelian, i would suggest a much lower Malt content...
Specially for slants...:shrug:

I personally recommend 1g ELME per 100ml dh2o


Edited by total (02/11/12 12:51 PM)


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Invisiblearis
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Re: Mephisto's Master Slant Tek [Re: Mephistophelian]
    #15792592 - 02/11/12 12:55 PM (12 years, 7 days ago)

What are the advantages of doing this with agar instead of distilled water?

http://www.shroomery.org/forums/showflat.php/Number/14632590#14632590

Quote:

lipa said:
Just some extra info. I always make backups with culture tubes and distilled water. It is important that the wedges be very small 3-4mm diameter and put 3-4 of them in each tube. You don't have to use cryo-vials either. I use transport tubes that are 5ml.

There is an advantage to making distilled water vials too.  If you make a few of them for each strain it is very easy to make grain spawn masters without having to get the mycelium running on agar first. Just make up some grain and drop the wedges strait into the grain and done.

I have been thinking about growing mycelium over small pieces of wood and dropping them in to distilled water as well. You could use cardboard most likely. I have also used those little kitty litter cardboard balls they sell at the pet store moistened and loaded in small tubes with mycelium. I recently pulled a three year old shiitake culture out of the fridge and placed a small chunk on agar. Took of like a rocket and looks really healthy. Agrocybe agertita stores really well this way too.

Don't expect distilled water storage to work every time. It doesn't with every species. That's why I do both so I will know which ones will work like that in the future (I also like the ability to just make up grain spawn masters without having to have an extra plate out).  Storage vials take up very little room. That's nice when you are limited on space
.
Lipa




Edited by aris (02/11/12 01:05 PM)


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Offlinetotal
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Re: Mephisto's Master Slant Tek [Re: aris]
    #15792656 - 02/11/12 01:13 PM (12 years, 7 days ago)

Distilled water storage is a 3d storage...
I prefer growth on agar b/c the culture grows on a 2d plane, it makes seeing contaminates easier...
And when your working with master/backup cultures, hidden contamination is the last thing you want...

:2cents:


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Offlinewinemaker
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Re: Mephisto's Master Slant Tek [Re: Mephistophelian]
    #26037311 - 06/06/19 09:07 PM (4 years, 8 months ago)

And today I learn what slants are. Thank you for the write-up!


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OfflineMephistophelian
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Re: Mephisto's Master Slant Tek [Re: winemaker]
    #26044779 - 06/10/19 08:54 PM (4 years, 8 months ago)

You are very welcome! Glad it could be of use even after all these years :laugh:


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Master Slants Made Simple


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