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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Do we always Flame between transfer? [Re: bodhisatta]
    #26032023 -

bodhisatta said:
LegionOfShroom said:
Whoa ok so here’s Bod saying no Flame until you switch cultures! Obviously having the clean culture and good technique to work with is crucial. I probably won’t attempt it when noccing up grain jars for awhile, but I’m going to start trying that with regular transfers. If I start getting random contams I’ll have to reconsider


Sometimes i do sometimes i Don't. If im making three transfers from one plate to three new ones i might not.
If im taking three transfers from the spore plate to three new plates i will flame so I do not cross contaminate genetics or possible contaminants


Exactly :whathesaid:


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: Do we always Flame between transfer? [Re: van hatton] * 2
    #26032039 -

well said. It becomes common sense after some time and practice. If i know that my culture is clean and know that my scalpel is still clean, i don't take the time to re flame it. I took five transfers to new plates last night without flaming between.

It is true that a scalpel can have invisibly tiny myc fragments on it that can come off in the agar and grow out. For this reason, if you are taking from multiple different genetics and don't want to mix them up, it is important to flame. As a rule of thumb though, a noob should just flame between every transfer until they've gotten really good at sterile transfers.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Do we always Flame between transfer? [Re: verum subsequentis]
    #26032045 -

verum subsequentis said:
a noob should just flame between every transfer until they've gotten really good at sterile transfers.


This should be said one more time. :cheers:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Extras: Filter Print Post Top
Re: Do we always Flame between transfer? [Re: van hatton]
    #26032055 -

A noob should just flame between every transfer until they've gotten really good at sterile transfers


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Do we always Flame between transfer? [Re: van hatton]
    #26032064 -

By flaming every time when something does go wrong It'll be easier to trace back to where the problem originated..
There's none of this: Did I flame or did I not flame?  I forget...!


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Re: Do we always Flame between transfer? [Re: verum subsequentis]
    #26032072 -

verum subsequentis said:
A noob should just flame between every transfer until they've gotten really good at sterile transfers


Man, I thought I was going to get lynched for saying I didn’t flame every time.  really consider myself a noob, just want to be efficient not lazy.  I don’t think I would suggest noobs sterilizing every time cause in my mind flaming causes more hand movement which causes more air movement, and for me a warm blade melts the agar and you lose more ability to hold your wedge, and maybe it cools on the blade causing it to stick when you want to drop it.  And maybe I am over thinking it.
Edit, I wrote in the context of agar to grain or good isolates a2a.  Doing 1st or even second transfer I would suggest flaming.


--------------------
Asante’s woodlover patch
Moricz cellar
D3’s agar tek
A woodlover genetic theory
holofracatl's Woodlover grows
Tweeq's woodlover grow
Liloldme's grow

Edited by Land Trout (06/04/19 09:32 AM)

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Re: Do we always Flame between transfer? [Re: verum subsequentis]
    #26032075 -

verum subsequentis said:
well said. It becomes common sense after some time and practice. If i know that my culture is clean and know that my scalpel is still clean, i don't take the time to re flame it. I took five transfers to new plates last night without flaming between.

It is true that a scalpel can have invisibly tiny myc fragments on it that can come off in the agar and grow out. For this reason, if you are taking from multiple different genetics and don't want to mix them up, it is important to flame. As a rule of thumb though, a noob should just flame between every transfer until they've gotten really good at sterile transfers.


I've been wondering about this, I also imagine that youd prolly have a much smaller available genetic material by using almost microscopic fragments to transfer.


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"There is never a wrong note, bend it." Jimi Hendrix


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Re: Do we always Flame between transfer? [Re: sui]
    #26032077 -

That's how the josex poke works. Fragments sticking to a heated needle tip.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Do we always Flame between transfer? [Re: Land Trout]
    #26032093 -

Land Trout said:
verum subsequentis said:
A noob should just flame between every transfer until they've gotten really good at sterile transfers


Man, I thought I was going to get lynched for saying I didn’t flame every time.  really consider myself a noob, just want to be efficient not lazy.  I don’t think I would suggest noobs sterilizing every time cause in my mind flaming causes more hand movement which causes more air movement, and for me a warm blade melts the agar and you lose more ability to hold your wedge, and maybe it cools on the blade causing it to stick when you want to drop it.  And maybe I am over thinking it.
Edit, I wrote in the context of agar to grain or good isolates a2a.  Doing 1st or even second transfer I would suggest flaming.


Take the extra time, flame your tool, and let it cool.

Once you have confidence in all aspects of it, Tool sterility, deftness of movements, eliminating any unnecessary contact with your tool to anything other than your sample.


--------------------

"There is never a wrong note, bend it." Jimi Hendrix


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Re: Do we always Flame between transfer? [Re: mushboy]
    #26032096 -

mushboy said:
That's how the josex poke works. Fragments sticking to a heated needle tip.


Link?


--------------------

"There is never a wrong note, bend it." Jimi Hendrix


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Re: Do we always Flame between transfer? [Re: sui]
    #26032107 -

Ur a mod and you dont know what the poke is :oldman:

:lol: jking here ya go.


https://www.shroomery.org/forums/showflat.php/Number/24740168/page/1


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: Do we always Flame between transfer? [Re: van hatton]
    #26032112 -

I'm meandering my way through it dagnab it.


--------------------

"There is never a wrong note, bend it." Jimi Hendrix


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Re: Do we always Flame between transfer? [Re: sui]
    #26032114 -

:lol:

The poke is awesome for lcs. And super dirty plates.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Extras: Filter Print Post Top
Re: Do we always Flame between transfer? [Re: van hatton] * 1
    #26032118 -

I still haven't tried the "poke". Maybe I'll give er a try but haven't found the need yet. I know it'll work though. I once did a series of transfer tests in which i just dragged the blade through the edge of a culture and then dragged it through a new plate. Those tests almost always grew out. I also did tests where i jest poked the tip of the scalpel into a culture and into a new plate. About 40 percent of the poke marks grew out.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Do we always Flame between transfer? [Re: van hatton]
    #26032119 -

van hatton said:
Ur a mod and you dont know what the poke is :oldman:

:lol: jking here ya go.


https://www.shroomery.org/forums/showflat.php/Number/24740168/page/1


Dank!


--------------------

"There is never a wrong note, bend it." Jimi Hendrix


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Re: Do we always Flame between transfer? [Re: verum subsequentis]
    #26032124 -

You only need a few cells of mycelium  to colonize most things. depending.

I did the poke directly to grain took.around 28 days for 100% including shakes.

But i mean shit if you can knock a grain jar you can knock an lc. :shrug:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Extras: Filter Print Post Top
Re: Do we always Flame between transfer? [Re: verum subsequentis]
    #26032126 -

it gives me such a hard on knowing these tiny invisible fragments can grow countless pounds:vibin::splooge:


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Do we always Flame between transfer? [Re: mushboy]
    #26032145 -

It's truly amazing. I'm starting to think that it's actually limitless. The other day I made SOOO much LC out of a nickle sized growth on agar and started thinking about what could really be achieved with one clean plate. Rough math...
1 poke per 1000 cc LC....
pokes available in plate.... ? let's say 100 even though it's way more.
100 pokes into 100 1000 cc LCs and your at 100,000 CCs of LC.
10 CCs per 4 quart bag of spawn and your at 10,000 bags
1/2 dry per bag over two flushes and you've got 5,000 lbs of magic.
5000 lbs from one plate. WHAT THE FUCK.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Do we always Flame between transfer? [Re: verum subsequentis]
    #26032149 -

Yeah ive been making my a2g smaller and smaller.
I do 8 jars from one 60mm now.



It could be even more than that as well.
And faster. 1 lc.to 1000 lcs.

overexpansion  could become an issue maybe.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Extras: Filter Print Post Top
Re: Do we always Flame between transfer? [Re: verum subsequentis]
    #26032152 -

You could colonize the entire universe starting with something you can't even see without a microscope.:pikannom:


--------------------
Asante’s woodlover patch
Moricz cellar
D3’s agar tek
A woodlover genetic theory
holofracatl's Woodlover grows
Tweeq's woodlover grow
Liloldme's grow

Extras: Filter Print Post Top
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Shop: North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale

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