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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Do we always Flame between transfer?
    #26031051 -

Is it standard to take a torch to your scalpel after every single transfer? For example if there’s 1 culture in a single dish and I want to take 3 transfers to 3 separate dishes flaming every time is not overkilll but is standard practice correct? All my work is performed in a flow hood

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Re: Do we always Flame between transfer? [Re: LegionOfShroom]
    #26031061 -

I dip in alcohol and then flame every time.


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"There is never a wrong note, bend it." Jimi Hendrix


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Re: Do we always Flame between transfer? [Re: sui]
    #26031079 -

I often wipe down with alcohol soaked paper towel too, dipping might be more efficient I’ll have to try that

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Re: Do we always Flame between transfer? [Re: LegionOfShroom]
    #26031186 -

+1 dip and flame. Probably overkill, but worth it.

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Re: Do we always Flame between transfer? [Re: MLPismyOPSEC]
    #26031192 -

I spray everything with iso and flame like it's the only thing keeping me alive. Better safe than sorry and whatnot.

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Re: Do we always Flame between transfer? [Re: Bph] * 3
    #26031201 -

keeping you alive until you ignite iso vapor. dont spray iso:oldman:


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Do we always Flame between transfer? [Re: Bph]
    #26031204 -

Flame only and it's worked every time.

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Re: Do we always Flame between transfer? [Re: Claybirdd]
    #26031214 -

The dip gets any sticky agar off the scalpol and just adds that bit extra. I've been having a problem with my alcohol lamp not getting my scalpol red hot, I haven't had many issues but I think a torch is better.


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"There is never a wrong note, bend it." Jimi Hendrix


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Re: Do we always Flame between transfer? [Re: Claybirdd]
    #26031245 -

I never make a transfer without the flame first and I never have broken my rules..


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Re: Do we always Flame between transfer? [Re: Claybirdd] * 1
    #26031264 -

Wipe it to get crud off if needed because it's not helping sterility. If you can see any glowing of the tool in regular ambient light while flaming it then the metal temperature is already over 1000°F.

And yes, flame after each transfer.


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"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna

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Re: Do we always Flame between transfer? [Re: LegionOfShroom]
    #26031301 -

LegionOfShroom said:
Is it standard to take a torch to your scalpel after every single transfer? For example if there’s 1 culture in a single dish and I want to take 3 transfers to 3 separate dishes flaming every time is not overkilll but is standard practice correct? All my work is performed in a flow hood


Yes, flame it red hot between each transfer.  You will have a notable increase in contams otherwise.


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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand


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Re: Do we always Flame between transfer? [Re: Psicomb]
    #26031308 -

I flame once and if I touch anything with the blade, and I don’t have problems.  And I’m sure that offends some people, but it works for me.


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Asante’s woodlover patch
Moricz cellar
D3’s agar tek
A woodlover genetic theory
holofracatl's Woodlover grows
Tweeq's woodlover grow
Liloldme's grow

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Re: Do we always Flame between transfer? [Re: Land Trout] * 1
    #26031471 -

This post is strictly if you have a clean culture and good technique.

I use a fh and will do 5-7 transfers between flames.

If i switch cultures i reflame.


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Edited by van hatton (06/04/19 12:01 AM)

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Re: Do we always Flame between transfer? [Re: van hatton]
    #26031746 -

:whathesaid:
Id do the same in a sab :shrug:


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Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Do we always Flame between transfer? [Re: bodhisatta]
    #26031827 -

Whoa ok so here’s Bod saying no Flame until you switch cultures! Obviously having the clean culture and good technique to work with is crucial. I probably won’t attempt it when noccing up grain jars for awhile, but I’m going to start trying that with regular transfers. If I start getting random contams I’ll have to reconsider

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Re: Do we always Flame between transfer? [Re: LegionOfShroom]
    #26031839 -

It's about common sense.

If your flamed blade touches a sterile culture, and you take a cut and put to another sterile dish your blade is still sterile because everything it touched is sterile. Like van said, if you switch cultures you flame so you dont cross contaminate cultures.

However, from an educational and instructional standpoint you ALWAYS flame.



--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Do we always Flame between transfer? [Re: LegionOfShroom]
    #26031844 -

I had one transfer last week where I lifted my dish over the jar and knocked the wedge in after it fell apart while trying to get it on there. Maybe it was too hot, but I said if one is contamed I'll know it's that one. One did contam, also I think the next one.

Deftness in movements is key.


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"There is never a wrong note, bend it." Jimi Hendrix


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Re: Do we always Flame between transfer? [Re: LegionOfShroom]
    #26031885 -

LegionOfShroom said:
Whoa ok so here’s Bod saying no Flame until you switch cultures! Obviously having the clean culture and good technique to work with is crucial. I probably won’t attempt it when noccing up grain jars for awhile, but I’m going to start trying that with regular transfers. If I start getting random contams I’ll have to reconsider


Sometimes i do sometimes i Don't. If im making three transfers from one plate to three new ones i might not.
If im taking three transfers from the spore plate to three new plates i will flame so I do not cross contaminate genetics or possible contaminants


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Do we always Flame between transfer? [Re: bodhisatta]
    #26031889 -

Watch my sab videos on agar work. I cut corners with flaming. Some people noticed. I did it so some people would catch on to the common sense part of it not the robotic following advice because I can't make my own decisions stuff.


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Do we always Flame between transfer? [Re: bodhisatta]
    #26031901 -

A good method is also to mark any jars where you notice a flaw in your technique, that way if you end up with problems you can easily reference back to the transfer and identify your vectors


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"There is never a wrong note, bend it." Jimi Hendrix


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