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Offlinewutang
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agar plate transfers..im not sure?? answers?
    #7661872 - 11/21/07 08:20 AM (16 years, 2 months ago)

i have a few plates that i was sure wasn't going to grow...
then i looked and there was mycelium growing..and there was
a bit of trich green mold too....

so should i transfer a SMALL piece of mycelium into another plate
and keep doing that until the contam doesn't follow? until its 100% colonized and contam free right?

an explanation would be good..i used a print on one
that was from a trade and it wasn't so sterile...so i figure I can
keep plate transfering until its clean?


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Offlinewutang
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Re: agar plate transfers..im not sure?? answers? [Re: wutang]
    #7662064 - 11/21/07 09:34 AM (16 years, 2 months ago)

bump


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Offlinewutang
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Re: agar plate transfers..im not sure?? answers? [Re: wutang]
    #7662069 - 11/21/07 09:36 AM (16 years, 2 months ago)

okay so heres an example of pan cyan agar..
it looks fucking gross and i want to try to separate the mycelia (3 white spots)
from the contams...i dont even know wtf that is!!



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OfflineRogerRabbitM
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Re: agar plate transfers..im not sure?? answers? [Re: wutang]
    #7662083 - 11/21/07 09:40 AM (16 years, 2 months ago)

Search using my username and keywords such as 'agar' and 'isolation from contaminants'. I've typed out detailed teks for isolating away from contamination several times, so they should be easy to find.

All I see is bacteria on that bottom jar. I'd suggest getting a few sleeves of disposable petri dishes.
RR


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Offlinewutang
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Re: agar plate transfers..im not sure?? answers? [Re: RogerRabbit]
    #7662087 - 11/21/07 09:42 AM (16 years, 2 months ago)

oh thanks


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Offlinewutang
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Re: agar plate transfers..im not sure?? answers? [Re: wutang]
    #7662146 - 11/21/07 10:02 AM (16 years, 2 months ago)

err...

I couldn't find anything in the SE
can you give me a link rr?

and i cant really find petri dishes were im at..
so im stuck using jars


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OfflineRogerRabbitM
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Re: agar plate transfers..im not sure?? answers? [Re: wutang]
    #7662239 - 11/21/07 10:27 AM (16 years, 2 months ago)

I can't post search results where you can see them. I just got over 50 threads by using 'search posts' at the top of this page, 'mushroom cultivation' as the forum, 'RogerRabbit' as the user, and agar isolate contaminants as the search terms. Do it.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinetotal
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Re: agar plate transfers..im not sure?? answers? [Re: RogerRabbit]
    #7662650 - 11/21/07 12:16 PM (16 years, 2 months ago)

Quote:

RogerRabbit said:
With a flame sterilized scalpel, transfer each new growth the minute you see it and can differentiate it from other growths.  Transfer each and every growth you see to its own petri dish.  This means you'll have some dishes that turn out to be 100% mold, but also dishes that will be pure mushroom mycelium, provided you can get a clean transfer. 

If you wait too long, the mold will sporulate, then when you transfer the good stuff, it will have mold spores attached, ruining the culture later on.

It should go without saying to use a glovebox.  If a flowhood is used, the airflow will blow the contaminant spores all over the place.  This is just a note to those of you who have just purchased or made a flowhood.  Never open a contaminated petri dish or jar in front of it.
RR





found that quote on the first one i clicked using the search details that RR provided...should have let him find it thou :shrug:


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