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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: I seriously don't get it. [Re: cronicr]
    #26027202 -

10kvissions:
It's possible that your poly fill filter and the Micropore tape acted like a wick giving the bacteria a path to contaminate your agar while it was wet/damp from the pc. cycle.
That's why I like synthetic filter disc. cut in quarter size and silicone onto the plastic lids..


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Re: I seriously don't get it. [Re: micelio]
    #26027220 -

I tried glass jar petri dishes, they are bad. However if you are getting that amount of contamination you may want to look at the innoculant. Are you getting syringes from your buddy, a sponsor, a random vendor? If that's happening from a print I must ask if you are using a utility knife smothered dumpster juice to make transfers. You are certainly dealing with sneaky ninjas or you are doing something completely wrong. Or using syringes full of trichoderma. Or your PC is broken. Have you tried making control blank plates?


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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Re: I seriously don't get it. [Re: Professor X]
    #26027224 -

How would a broken PC effect trich problems lol


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: I seriously don't get it. [Re: cronicr]
    #26027247 -

10kvission said:
UPDATE:

WBS Plain (no knock) jars
so far the jars aren't growing anything both PT and Polyfil jars look the same. so thats good news.  at least i know the PC'ing is doing its job.

Agar
The Polyfil jars are developing faster than the PT jars are for sure. i've seen the same ammout of growth that took 5 days in the PT jars happen in only 2 days in the Poly jars.  agar is the same... technic, PC all that is exactly the same.
Here are the Agar Photos so far.
-------------------------------------------
He did not inoculate those plates...
I assume the dishes where pull out of the pc. and place on a shelf for three to four days.. Now I wonder how did that much bacteria get in every plate?
He did say he was going to do a test run and compare the two different filter types.
Maybe I'm reading it wrong and he did inoculated those dish.


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Edited by micelio (06/01/19 02:15 PM)

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Re: I seriously don't get it. [Re: Professor X]
    #26031323 -

Professor X said:
Sure doesn't look like cube myc. It looks like trichoderma. What are you inoculating with? Might want to start completely fresh, new prints.

By the way, waaay too much agar in those dishes. Just barely cover the bottom.



that is just covering the bottom.  the jars dome up on the bottom so im just covering enough to cover the middle (top of dome) which means the edges are going to look more full.


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Re: I seriously don't get it. [Re: micelio]
    #26031328 -

micelio said:
10kvission said:
UPDATE:

WBS Plain (no knock) jars
so far the jars aren't growing anything both PT and Polyfil jars look the same. so thats good news.  at least i know the PC'ing is doing its job.

Agar
The Polyfil jars are developing faster than the PT jars are for sure. i've seen the same ammout of growth that took 5 days in the PT jars happen in only 2 days in the Poly jars.  agar is the same... technic, PC all that is exactly the same.
Here are the Agar Photos so far.
-------------------------------------------
He did not inoculate those plates...
I assume the dishes where pull out of the pc. and place on a shelf for three to four days.. Now I wonder how did that much bacteria get in every plate?
He did say he was going to do a test run and compare the two different filter types.
Maybe I'm reading it wrong and he did inoculated those dish.


yes your reading it wrong.  i inoculated all the plates.  i did NOT inoculate the jars.


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Re: I seriously don't get it. [Re: 10kVisions]
    #26031345 -

everyone has such contradicting opinions and this is the issue with this forum.  according to most my step by step process (following pasty white) is perfect.  heat, PC times (adjusted for altitude etc) sterile environments etc... now suddenly after everyone has said "that looks right" "thats teh correct way to do it" now suddenly its wrong.  and this would be another reason for this post.  i really don't get it.

i have myc growth on 2 of the jars.  little hair like growth that is very uniform and nice looking.  both jars have the polyfill.  the PT jars all have the "circle of death" as i like to put it.  its obviously mold.

how is my technic wrong?
i PC for 45 min at 19psi (correct according to quite a few on here including bodhi)
my jars come out and go straight to my SAB siting overnight to cool.
i then use a syringe knock into each jar heating between each pour in each jar, using 2 drops.
use a Loop tool to spread that is soaked in alcohol and also heated between each jar.
lid goes back on and stays in SAB until all jars are complete.
jars then sit on a shelf at a steady 71º.  so where did i go wrong?
gloves, warn
arm sleeves, warn
the only thing missing would be a painters mask but my SAB holes are small enough that that shouldn't be an issue.

for the record. the syringes i get from "ralph" for those of you who know then you know he's a trusted source.  I've had syringes from 4 different places and no luck with anything thus far (in  the new house)

the new images in my signature are from my old grows.  forgot i took them.. but its just also nice to have proof that I've done this with NO issues in the past.


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Re: I seriously don't get it. [Re: 10kVisions]
    #26031368 -

I noticed you left some uninoculated grain jars out as a test. Did you leave an uninoculated agar plate as a test? That'd rule out agar prep. Your technique is sounding pretty good. If that truly is sound, then it could be the spore syringe?


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First Grow

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Re: I seriously don't get it. [Re: Raccoon]
    #26031377 -


These are the 2 i think have "good" spots in them.  am i wrong?  won't i be able to transfer this away from the bad.  isn't that the point of agar?

the rest all look like shit.  they smell like dirty feet too.  never smelt that before. i have now tossed all jars except the 2 i think are worth keeping.



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Re: I seriously don't get it. [Re: 10kVisions]
    #26031389 -

Try to transfer. It looks pretty bad though... Good luck.

One thing I just thought of, you're using too much solution. Don't squirt directly into the jar. Flame the needle and inoculation loop, squirt some on the sab floor to cool the needle, squirt some on the loop to cool the loop, then squirt a bit more to load the loop with less than one drop, streak the plate, call it a day. You'll have much less bacteria and myc, which will be easier to separate.


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First Grow

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Re: I seriously don't get it. [Re: 10kVisions] * 1
    #26031390 -

I wouldn't trust those. Doesn't look like myc to me. Obviously there's tons of bacteria too. You need better innoculant man. Hella dirty.


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contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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Re: I seriously don't get it. [Re: Mad Season]
    #26031397 -

Raccoon said:
Try to transfer. It looks pretty bad though... Good luck.

One thing I just thought of, you're using too much solution. Don't squirt directly into the jar. Flame the needle and inoculation loop, squirt some on the sab floor to cool the needle, squirt some on the loop to cool the loop, then squirt a bit more to load the loop with less than one drop, streak the plate, call it a day. You'll have much less bacteria and myc, which will be easier to separate.


ok thats interesting.  haven't read that technic.  i am heating both loop and needle and i am squirting out a few drop into the sab to cool the needle but all teh teks say place a drop or 2 onto the agar and to use the loop (after cooling in agar) to spread those drops.

Mad Season said:
I wouldn't trust those. Doesn't look like myc to me. Obviously there's tons of bacteria too. You need better innoculant man. Hella dirty.


so what does it look like?  not the huge gross whit shit but the light uniformed growth coming of the edge.  what would it be?


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Re: I seriously don't get it. [Re: 10kVisions]
    #26031428 -

:shrug: just really wispy looking. Not what I'd desire at all. The first pic might be alright, but again it's still pretty wispy, but that 2nd one is :thumbdown:

Have you seen my hidden contams thread? At the end of the op, I talk about what is desirable when selecting grain inoc.
https://www.shroomery.org/forums/showflat.php/Number/22020260

I just know you'll have a much easier time with cleaner spores. Get a print. Fuck syringes when it comes to agar. Just my opinion.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Edited by Mad Season (06/03/19 11:12 PM)

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Re: I seriously don't get it. [Re: 10kVisions]
    #26031429 -

Apparently, for everyone else you don't need much to get tons of colonies. My experience, however, I got only like 7 myc colonies on 20 plates or so using this technique :shrug: That's enough for me, but I'm curious on why I have such low germination rate...


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First Grow

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Re: I seriously don't get it. [Re: Raccoon]
    #26031467 -

you need a print and make yourself an inoculation loop with a vape coil. if you wanna try again with a dirty syringe try the brf puck method for getting away from bacteria.

you might wanna try the marketplace for a print. good luck.


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: I seriously don't get it. [Re: JHOVA]
    #26031478 -

What I suggested: get some new spores. Spore prints. Not spore syringes.

I live in a house that full of mode spores.
This is how I keep my prints lc. and tools clean. 24 7..

https://www.shroomery.org/forums/showflat.php/Number/26018520#26018520


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Re: I seriously don't get it. [Re: micelio]
    #26031487 -

Mad Season said:
:shrug: just really wispy looking. Not what I'd desire at all. The first pic might be alright, but again it's still pretty wispy, but that 2nd one is :thumbdown:

Have you seen my hidden contams thread? At the end of the op, I talk about what is desirable when selecting grain inoc.
https://www.shroomery.org/forums/showflat.php/Number/22020260

I just know you'll have a much easier time with cleaner spores. Get a print. Fuck syringes when it comes to agar. Just my opinion.


thanks for the link i'll get on that reading first thing tomorrow.

Raccoon said:
Apparently, for everyone else you don't need much to get tons of colonies. My experience, however, I got only like 7 myc colonies on 20 plates or so using this technique :shrug: That's enough for me, but I'm curious on why I have such low germination rate...


making new agar plates first thing in the morning i have 4 more syringes 2 types each and i'll try your Tek with the new agar plates.

micelio said:
What I suggested: get some new spores. Spore prints. Not spore syringes.

I live in a house that full of mode spores.
This is how I keep my prints lc. and tools clean. 24 7..

https://www.shroomery.org/forums/showflat.php/Number/26018520#26018520


im all about getting spore prints not syringes.  how can i go about this?


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Re: I seriously don't get it. [Re: 10kVisions]
    #26031494 -

If you live in Ca. you would have to go out of state. or Idaho or Georgia..

I have a Shroomery sponsor I order from.


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Re: I seriously don't get it. [Re: 10kVisions]
    #26031497 -

See C10 agar thread for a good way to streak and increase chances of separating from contams. Scroll way down, it's very thorough.

The cleanup method Jhova mentioned seems interesting too. If streaking in agar doesn't work, I'd totally try that. And in the meantime, good luck getting spores. I'd send you some, but my situation makes it difficult...

JHOVA said:
if you wanna try again with a dirty syringe try the brf puck method for getting away from bacteria.




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First Grow

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Re: I seriously don't get it. [Re: Raccoon]
    #26031522 -

I just read through the entire thread start to finish.
So it looks like the bacteria is following your streak pattern to me this means poor sterile technique, dirty inoculation loop or else contaminated innoculant.
Try it like this.
Wipe jar and lid with iso.
Wipe syringe with iso.
Flame syringe needle.
Lift one corner of the lid but don't take it all the way off squirt in one drop 2 tops under the lifted corner of lid without touching the needle to lid or jar edge. Don't even bother squirting out a drop to cool the syringe first. Replace lid and leave it don't use a loop.
Report back with the results if you do it this way and you still get a plate full of bacteria it's either plate preparation which I doubt this is it or it's the innoculant. If you want to rule out plate prep do a 2nd plate from the same batch and leave it untouched if it doesn't contam it's your innoculant.
At least in my head this would narrow it down through process of elimination.
Let us know how it goes and good luck.

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Shop: Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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