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Amazon Shop: ½ Pint Jars, 10cc Syringe, Agar, Parafilm

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InvisibleYidakiMan
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Storing mycelium on distilled water...
    #2589415 - 04/22/04 01:44 AM (13 years, 3 months ago)

I was looking for vials and I came across exactly what I was looking for.
http://www.cynmar.com/product_info.php?products_id=1033

Forty nine 1 dram vials, w/ rubber lined caps. And a neat little box too. At four vials per stock culture it would store 12 cultures nearly indefinately. =)

Think I might get me one.


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Offlinezeronio
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Re: Storing mycelium on distilled water... [Re: YidakiMan]
    #2589487 - 04/22/04 02:12 AM (13 years, 3 months ago)

23$ for 49 vials is rather expensive. I tried those and I didn't like their narrow mouth. It's difficult to get the mycellium out of them.

Now I use 2ml  microcentrifuge polypropylene tubes that cost about 30$ for 1000 vials.  :cool:


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Offlineexprtcontamgrwer
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Re: Storing mycelium on distilled water... [Re: YidakiMan]
    #2589531 - 04/22/04 02:24 AM (13 years, 3 months ago)

What kind of luck has anyone been having storing mycellium in distilled water? And for how long? I've read that it works great and that it doesnt work at all. Which is it?


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Offlinecreekfarmer
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Re: Storing mycelium on distilled water... [Re: exprtcontamgrwer]
    #2589927 - 04/22/04 07:06 AM (13 years, 3 months ago)

It works great. If it does'nt then the fault is in poor lab technique. I just use jelly jars.


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InvisibleYidakiMan
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Re: Storing mycelium on distilled water... [Re: creekfarmer]
    #2590300 - 04/22/04 10:55 AM (13 years, 3 months ago)

Oh cool. I actually have some of those tubes laying around.... The local science surplus store sells 'em for 5 cents a pop. You don't have any problems with leaks or evaporation?


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InvisibleYidakiMan
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Re: Storing mycelium on distilled water... [Re: YidakiMan]
    #2590405 - 04/22/04 11:36 AM (13 years, 3 months ago)

I tried it once with a monokaryotic strain. It didn't quite go well, I was kinda confused on the technique. It didn't reanimate on agar. This time I plan on taking a larger amount of mycelium from my plate. I'll store a few tubes and use one or two to start some liquid culture.
I bought an eberbach container at a real deal off eBay, but I don't have a base yet. This is the way I will be making my fermented mycelium until I get a Waring blender base for cheap.


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Offlineexprtcontamgrwer
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Re: Storing mycelium on distilled water... [Re: creekfarmer]
    #2593149 - 04/23/04 01:10 AM (13 years, 3 months ago)

creekfarmer could you describe the way you do it that works so well?


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InvisibleYidakiMan
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Re: Storing mycelium on distilled water... [Re: exprtcontamgrwer]
    #2593403 - 04/23/04 02:25 AM (13 years, 3 months ago)

I did it again tonight and I bet it worked.

I sterilized 10cc of water in a syringe. Once cool, I flamed a needle and squirted drops of water forming a ring with a diameter of 3 cm. around the center of the plate. Using the needle I scraped at the mycelium while sucking up water. I see chunks of mycelium in the water, but I bet there are hundreds of fragments invisible to the naked eye. I already had the 2ml centrifugal tubes that creekfarmer mentioned. The tubes were not sterile so I put one drop of household h2o2 in followed by 1.5ml of mycelium water. Even though I started with 10cc I never got more than 8ml back into the syringe. The King Oyster and regular Oyster were from fully colonized plates and I only used one plate per syringe. Tonight my furthest advanced Elm Oyster was only 5 cm across. I used that and another plate that had similar sized growth on it. This is of course a huge cross-contamination risk, but I am working intensively with the elm oyter and have more plates of elm oyster to fall back on.
So basically, from each plate, I got four vials of culture that will not need refridgeration and will not deteriote and are immediately ready for storage without incubation.

If it doesn't seem to be working, practice more and you'll get the technique down.


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Offlinezeronio
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Re: Storing mycelium on distilled water... [Re: exprtcontamgrwer]
    #2593659 - 04/23/04 04:09 AM (13 years, 3 months ago)

I lost some cultures because of contamination but mostly it works great. You have to be very careful when you make them to keep everything absolutely sterile to wrap the vials with parafilm even if they seem to be tightly closed.


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Offlineexprtcontamgrwer
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Re: Storing mycelium on distilled water... [Re: zeronio]
    #2596391 - 04/23/04 11:36 PM (13 years, 3 months ago)

what's the longest anyone here has stored a culture this way without problems?


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Amazon Shop: ½ Pint Jars, 10cc Syringe, Agar, Parafilm

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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