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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Golden Cocks [Re: cronicr]
    #25810141 -

cronicr said:
The second was doing seperate plates and the second i seen any mycelium it was put to a plate on top of eachother, the third was using isolates  which almost always just formed a trump wall keyword almost😉
These results stem from the second method though .



Excellent work, cronicr! So in essence this was done by putting together monokaryon isolates.


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Re: Golden Cocks [Re: denger]
    #25810153 -

No that would be the third way. By the time you see mycelium on a dish its long been dikaryotic if you streaked out spores


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Re: Golden Cocks [Re: bodhisatta]
    #25810160 -

bodhisatta said:
No that would be the third way. By the time you see mycelium on a dish its long been dikaryotic if you streaked out spores


That depends on the density of the streak. If it shows single colonies with some distance between them they could be monos with some probability.


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Re: Golden Cocks [Re: denger]
    #25810188 -

Yeah it is kind of tricky to isolate monos but not impossible


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Re: Golden Cocks [Re: bodhisatta]
    #25810192 -

Very cool :trippy:

Its so fun to watch all these different crosses people are doing. :ohwow:
10 years from now there are going to be so many more cubes just from people on the shroomery!


bodhisatta said:
Bro you need rattlesnake venom


:kittylaugh:


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Re: Golden Cocks [Re: denger]
    #25810284 -

denger said:
bodhisatta said:
No that would be the third way. By the time you see mycelium on a dish its long been dikaryotic if you streaked out spores


That depends on the density of the streak. If it shows single colonies with some distance between them they could be monos with some probability.


I purposely diluted spores down quite a bit then streaked them. Looked at with a microscope there's still just too many spores. And spores like to clump. If you could dilute spores and also keep them from grabbing a partner then maybe. But even using jet dry or other surface tension agents wasn't able to get spores broken apart enough to get monokaryotic growth no matter how hard I tried. I think it would take a micromanipulator scope to achieve single spore monokaryotic growth reliably

Even with hefty agitation you'll see clumps like this


Diluted and streaked


If you made a 10cc syringe with the smallest speck of spores you can see its still too many spores


Even at the hardly visible growth phase they're long dikaryotic from streak plates(not super diluted in this case)



Ive played around a bunch with germination and trying to get monokaryotic growth but i think its out of the reach of hobby equipment


Spores clump like madmen despite your best efforts to make them disperse evenly


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Re: Golden Cocks [Re: bodhisatta]
    #25810311 -

That depends on the density of the streak. If it shows single colonies with some distance between them they could be monos with some probability.


I purposely diluted spores down quite a bit then streaked them. Looked at with a microscope there's still just too many spores. And spores like to clump. If you could dilute spores and also keep them from grabbing a partner then maybe. But even using jet dry or other surface tension agents wasn't able to get spores broken apart enough to get monokaryotic growth no matter how hard I tried. I think it would take a micromanipulator scope to achieve single spore monokaryotic growth reliably

Even with hefty agitation you'll see clumps like this


Diluted and streaked


If you made a 10cc syringe with the smallest speck of spores you can see its still too many spores


Even at the hardly visible growth phase they're long dikaryotic from streak plates(not super diluted in this case)



Ive played around a bunch with germination and trying to get monokaryotic growth but i think its out of the reach of hobby equipment


Spores clump like madmen despite your best efforts to make them disperse evenly


Holy Balls! I have to get a microscope. This is badass, I was worried about the cotton tip I did. Not anymore.


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Re: Golden Cocks [Re: Mr. Funguy]
    #25810321 -

All you really need is the microscope and to constantly check for germination for quick transfers.


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Re: Golden Cocks [Re: cronicr]
    #25810323 -

Sounds easy in theory but its fucking hard😂


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It doesn't matter what i think of you...all that matters is clean spawn
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I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Golden Cocks [Re: cronicr]
    #25810356 -

Could you grab one of those spores with a real fine filament?what if you use jet dry water AND an agitator?

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Re: Golden Cocks [Re: Thesunbeam]
    #25810364 -

Thesunbeam said:
Could you grab one of those spores with a real fine filament?what if you use jet dry water AND an agitator?


Dilution would be easier.
Thankfully cubes do not know that they are penis envy or golden halo so simply mixing spores gives you a 1 in 4 chance lol


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It doesn't matter what i think of you...all that matters is clean spawn
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I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Golden Cocks [Re: Thesunbeam]
    #25810375 -

Thesunbeam said:
Could you grab one of those spores with a real fine filament?what if you use jet dry water AND an agitator?


Type sporeplay singer dissection microscope into YouTube


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Re: Golden Cocks [Re: bodhisatta]
    #25810486 -

Whoa that thing is awsome! And yeah that’s  exactly what I was thinking of.

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Re: Golden Cocks [Re: bodhisatta]
    #25810516 -

Any idea how much one of those retails for? I checked their website and couple other retailers that sell them just out of curiosity, but they all just say 'Request a Quote'. Obviously not a good sign price wise.


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Re: Golden Cocks [Re: vikingsc]
    #25810539 -

5-15k


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Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
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Re: Golden Cocks [Re: bodhisatta]
    #25810567 -

Yikes. Keep dreaming I guess.


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Re: Golden Cocks [Re: vikingsc]
    #25810579 -

So if you take 1 spore of one mushroom strain and 1 spore of another, you cross breed the two strains?


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Re: Golden Cocks [Re: MaxRebo]
    #25810581 -

No i simply swiped enough spores and let them do the work


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It doesn't matter what i think of you...all that matters is clean spawn
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I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Golden Cocks [Re: cronicr]
    #25810588 -

So why don't you just take that one golden cock, clone it, and make a golden monococktub?


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Edited by MaxRebo (02/13/19 04:42 PM)

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Re: Golden Cocks [Re: cronicr]
    #25810599 -

You would only need to go that route if you wanted certainty from the start.
Say crossing GT an B +, these pheno types are pretty easy to spot a cross however and using a lot of diversity can give you more options.
As you can see with the AA + PE I got the luecistic fruit and bumpy stipe but not the luecistic penis i wanted so hopefully on another substrate i may get it


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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