Johnny Dont said:

Yes the agar is suppose to stiffen after you pour it. I stack my dishes 20 high and let them cool like that. It helps with condensation

15 mins is probably enough time, if when you poured you could handle the agar.

I use a paper clip bent into a loop, not sure what a standard size is. But as long as you cooled it on a dish first and it stopped hissing, you should be fine

Germination can take 3 days to 3 weeks.

Its probably turned black from the smoke. As long as nothing is growing don't worry.

You should practice the movements in your SAB, to get a better feel. I like to record myself from above, so I can look and see what I did wrong

I wrap my dishes with plastic wrap and store them in my SAB in my closet. Mostly so they are out of the way. I'm sure you could just leave them on a shelf or whatever. But you should wrap them either with parafilm or plastic wrap

Hope that helps

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Nimph said:

Have you smelled the mushrooms were u got the spores from before? You could clean a plate a couple times on the limited choices an smell it but would just restart an norrow your vectors.



Move you hands really slow like a chameleon, the videos on here of people doing it are to fast.
Barely open it no more than u need very slow so it doesnt suck air in an flame everything that goes over your media.
Also let or make condensation go away before using them, thats were alot of infections around the edge come from an the excess water spreads it when its moved around. You wanna leave plates be untill your sure nothing else is in there(spore plates are always dirty.)

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Raj_5432 said:

hey all,

just an update, me still struggling with white mold contamination in agar plates, even after 4 trf i am left with only 1 agar plate which is clean.
Out of around 25 plates i managed only 1 which was clean. This even after taking utmost care what i learned here.

Also i did trial inoculation of wheat grain . Its 15 days now . But i am not 100 % sure its myc or white mold . Initially i confused mold for myc in agar plates, and it took me some time to know the diff.

Now i need opinion on my grain jar.Pls look at the pic and pls comment.

Also pls direct me to some good threads on contamination in jars, specially contamination which looks similar to myc.

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