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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25368891 -

verum subsequentis said:
questions are fine, i don't know the answer to that. the stuff i have does not say organic on it and it works quite nicely.


Alright, thanks

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Re: Penis envy isolation part 3 [Re: mushboy]
    #25368962 -

mushboy said:
you have a small hood and lots of penis envy?  :smilingpuppy:


:okthatsfunny:


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Re: Penis envy isolation part 3 [Re: Tormato]
    #25369197 -

ha!

just finished up g2g on 24 bags. mixed and leveled them and the last batch is cooling in the pc. tomorrow i'll noc up the rest.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25372028 -

verum subsequentis said:
ha!

just finished up g2g on 24 bags. mixed and leveled them and the last batch is cooling in the pc. tomorrow i'll noc up the rest.


Niiiiiiiiiiiiiiiice


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Penalties against drug use should not be more damaging to an individual than the use of the drug itself. Nowhere is this more clear than in the laws against the possession of marijuana and mushrooms in private for personal use.

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Re: Penis envy isolation part 3 [Re: TheDrugStore]
    #25373477 -

Man Verum, continuing to kill it.  That operation is HUGE!  Keep up the amazing work and thanks for documenting.  Hoping to get some PE cultures started from MS syringes soon.


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Ginge



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Re: Penis envy isolation part 3 [Re: Ginge]
    #25400740 -

So curious to the input output ratio. Probably going to be insane.


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Novice mycologist, my first inoculation was 8/1/18.

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Re: Penis envy isolation part 3 [Re: Bearded Irishman]
    #25400812 -

:cokeitch:

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Re: Penis envy isolation part 3 [Re: Skipio]
    #25404354 -

Heya,

How's it going, the bags all collinized?

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Re: Penis envy isolation part 3 [Re: Letsgrowstones] * 2
    #25405310 -

it's coming along all right but i underwent a pretty major hickup on my second round of bags. I'm not sure exactly what happened but about half of them did really weird shit. and the other half (or so) are cased and will be put into fruiting conditions today or tomorrow.

the first batch is done and most of them sucked fat balls. a few put out nicely and one has a very unique pheno that gave them all super wavy caps. I've been busy with all manner of things lately and haven't gotten around to uploading photos for an update but i will.

I'm not tripping to hard on it though because i've decided that i tried to do too much at a time and let my quality control slip a bit. i've decided that i'm going to do another round in shoeboxes @ something like 1q spawn to 2q coir. i'll just pull slants out 20 at a time, put them to plates, transfer to ensure cleanliness, put to grain (quarts) and then to shoeboxes.

i haven't decided yet if i'll do that with all of them or just the ones that showed potential. i'm a little torn because i fully expect many of the isolates to be shitty but i've also had a bunch of my best clones perform horribly in bags lately so i don't know for sure that they were genetically shitty.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25405521 -

:popcorn::takingnotes:


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🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: Penis envy isolation part 3 [Re: Caps McGee]
    #25439114 -

Your honesty is commendable. Nice job V.


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Re: Penis envy isolation part 3 [Re: NothingsChanged]
    #25439409 -

Deceit helps no one: least of all ourselves (which is a joke in and of: for  we are ALL "ourselves")


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🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: Penis envy isolation part 3 [Re: Caps McGee]
    #25462257 -

Been a little bit, curious how this is coming along for you bud.


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Space Ape Project
Links For Getting Started
Current PF Tek
Shoebox Assembly
All The Agar Info You Could Want
How It Should & Shouldn't Look


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Re: Penis envy isolation part 3 [Re: LtLurker]
    #25464505 -

LtLurker said:
Been a little bit, curious how this is coming along for you bud.


:snoopyes:


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🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: Penis envy isolation part 3 [Re: Caps McGee] * 1
    #25464560 -

well, i've now run every culture once. each was done in a bag that was two quarts cvg and two quarts grain that was nocced up via g2g with an entire quart. after the g2g they were three quarts spawn and two quarts cvg. this is a really high ratio but it had been working really well for me with Penis envy and a couple different non mutant varieties.

the isolates did not do well at all. most performed very poorly. i mean no yield at all. some refused to even begin to knot up and others looked very promising and just refused to mature. some had great pinsets that all aborted. a few produced half assed and a few produced relatively well.

i'm going to run them again in shoeboxes at a much lower spawn to sub ratio and see what happens. i'm not sure yet but i'm beginning to think that MAYBE PE doesn't do well isolated. who knows, it is a finicky little mutant bitch. maybe it needs a diverse communal pool of genetics to do what it does. i doubt it but it is possible.

i know that a lot of folks will assume that the conditions were off or my sterile tek was flawed but i am not new to either of those things and the sheer number of cultures tested would seemingly ensure a couple of good bags. it's possible that they didn't like the high spawn to sub ratio. we'll see how they do in shoes and take it from there.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis] * 1
    #25464574 -

verum, this reminds me something I have been thinking about a lot lately. When I was a noob,
everything was MS > LC or MS > WBS. I had some fantastic grows along with some epic failures
obviously.

When I started doing agar, I would usually transfer 3-4 times. Everything was clean but the
results weren't that great.

I've been thinking about just using T1 plates from now on, if things look good. I think you
want as much genetic variety as possible.


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Cultivating Panaeolus cyanescens


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Re: Penis envy isolation part 3 [Re: Asura] * 1
    #25464687 -

I think finding a good isolate is like the lottery, and seems multiple strains acting as a monoculture out performs most isolates


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🍄💚😎

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: Penis envy isolation part 3 [Re: Caps McGee]
    #25464707 -

That's interesting man. Thanks for the update.


--------------------
Space Ape Project
Links For Getting Started
Current PF Tek
Shoebox Assembly
All The Agar Info You Could Want
How It Should & Shouldn't Look


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Re: Penis envy isolation part 3 [Re: LtLurker] * 1
    #25464771 -

it's pretty well known that most isolates suck and that you'd have to try out a good couple to find the powerhouse. i FULLY anticipated that. i believe RR used to say that one in twelve isolates would "knock your socks off". that's why i went with sixty some odd.

the best way to quickly find powerful and reliable genetics is to clean up on agar and grow. clone a few of the best and grow. slant the best and grow it until the cows come home.

i'll know if these are trash once i run them all in shoeboxes. i already know i have a few with great potential and one that put off a really interesting wavy cap pheno that i'd keep growing just for shit's and grins.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25468592 -

Beautiful!


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https://troutsnotes.com/pdf/C2_CactusCultivation.pdf
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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