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Re: Penis envy isolation part 3 [Re: verum subsequentis] * 1
    #25343269 -

This is how history is made. What is being accomplished now is paving the way for the future. Very good work :thumbup:


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Re: Penis envy isolation part 3 [Re: YoYoSpores]
    #25343277 -

ahh shucks. thanks it's been quite the process.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25343385 -

Nail biter!

:mushroom2:

Can't wait!

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Re: Penis envy isolation part 3 [Re: Skipio]
    #25343659 -

Holy crap, you got a shroom factory, lol. Your badass Verum.


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My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.

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Re: Penis envy isolation part 3 [Re: BigSurMoon]
    #25347106 -

Holy fucking fuck verum!  This is amazing, I just browsed the thread and am in awe at how much time, work and energy you have put into this, I cannot wait to see you reap your due rewards, err fruits!  I just started in mycology and this is inspirational.  Very nice work, and very well documented.  I appreciate what you have done, although I am wondering how many of the plates I have I am going to truly try and isolate now :eek:


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Ginge



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Re: Penis envy isolation part 3 [Re: Ginge]
    #25347112 -

i'm glad you are enjoying it! what do you mean by
Quote:
Ginge said: although I am wondering how many of the plates I have I am going to truly try and isolate now :eek:



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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis] * 3
    #25347124 -

updated op with

BEHOLD







it is time to shake that ass



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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25347157 -

:grin::mushroom2::crazy2: def interested to see in the outcome!


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The thing about deceit is, you don't know your being deceived...

The only thing I am sure about is that I know nothing - socrates

Believe nothing you hear and half of what u see.

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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25347562 -

verum subsequentis said:
i'm glad you are enjoying it! what do you mean by
Quote:
Ginge said: although I am wondering how many of the plates I have I am going to truly try and isolate now :eek:



I simply had no idea the amount of resources, time, etc that were going to be needed to truly complete an isolation journey.  I have only been doing mycology about a month.  I MS noc'd up 16 pasty plates with GT and 16 with Treasure Coast, had 100% colonization with GT, 50% with TC, about to give up on the 4 week old TC's with no growth, but I am frugal and hate to waste, and I have no idea the condition of vendor spores.

Anyway, I am on the 3rd transfer GT and 2nd TC.  GT is like a dandelion and people have success with all sorts of genetics, should I even isolate a MC?  TC seems to be a bitch according to the forums, it would probably be worth the work, alternatively.  However, I think this work is much more worthy on PE or P. azure or something of the like.

To actually do this well I at least need petries, and a flow hood would be ideal.  I can continue in pasty plates and SAB for now but the size (were those 100 petries you used?) of petries helped so much in seeing your work that I think it is worth using.

Like you, I was originally saving plates to look at growth and because I am frugal and that shit is still viable! and when plates fully colonized I am experimenting with A2G with the remaining agar and myc.  Have 12 quarts and 5 1/2 pints going, so I can hopefully have some fruits with my science soon.

Side note, you changed your signature.  The jar in it, is the yellow circle the visible part of the agar disk from your petrie?  I cut my agar into 5 or 6pieces before noc'ing the grain, if that is what your image is, you kept it whole.  Is one or the other recommended for healthier or faster growth?

Keep up the good work, sorry for the wall of text!


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Ginge



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Re: Penis envy isolation part 3 [Re: Ginge]
    #25347583 -

I have another question...I was going to PM you but I remembered that other's may have the question and seek the answer as well.

You experimented with, and went back and forth with 3 cultures vs 1 culture for isolation.  Obviously at the end of the isolation 1 culture is ideal.  If you had it to do again, would you keep the larger petries 100-110 that it looks like you are using and keep the 3 culture strategy early, migrating to 1 culture as dictated by growth?  Or use the 50-60 petries and go single culture per plate the whole way?

PS If that is confusing to you, ask your friend with the top to bottom left to right thing to read it, I agree with him/her:wink:


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Ginge



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Re: Penis envy isolation part 3 [Re: Ginge]
    #25347745 -

just want to follow this great work! you've got a terrific level of ambition verum!!:jah:

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Re: Penis envy isolation part 3 [Re: k5hd2y] * 2
    #25348033 -

most folks nowadays would advise you to put your energy into getting a stellar clone. clones can kick absolute ass and are very easy to come by. while your ms tub(s) is growing your potential clones out you can continue thinning the genetic herd on agar.

how much spore solution did you put to each plate and did you streak them? the next time i do an isolation i'm going to dillute the ever living balls out of a syringe (or print or swab) and then streak i drip across each plate. the fewer spores you start with the quicker a single set of genetics can be isolated.

yes, i use 15x100 petris and i'd recommend doing three cultures per plate or you'll burn through plates like a mutha. i prefer them to pasty plates because of the visibility and ease to work with. i order celltreat dishes of amazon for 80 bucks a case (500).

what you see in my sig is the myc crawling off the agar and onto the grain. it's one of my isolates that i just thought looked sexy. i usually cut them up into multiple pieces but in this particular sterile session i had about fifty different cultures to put to slants and to grain and i couldn't be bothered to slice them all up. honestly though, i doubt it matters much. no matter how many pieces you are cutting up you are still introducing the same amount of myc so...:shrug: i suppose you'll get a little faster growth with five little pieces but i just let those grow out a couple days and shook the dog shit out of them. this breaks the agar into dozens of little pieces and distributes colonized grain all over the place. it's all over but the cryin now.

and to reiterate i'd go multiple cultures per 100 mm plate. possibly all the way. i had thought that it'd be better to let them grow out more so that i could more clearly see sectors but having experienced what i've been through i'd focus less on identifying sectors and more on saving plates and thinning the heard more quickly.

basically i found that there was WWAAYY more going on in each transfer than i had thought because there were way more spore on my germ plates than i had thought. so, i'd transfer until you've been seeing very obvious sectoring for a while and then divide them into how many plates you desire to isolate.

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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25348080 -

How long has your project gone? The original post is 10/17, but you were around t20 already.

It's gonna be awesome to see how these fruit. Good stuff man. :congrats:


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Space Ape Project
Links For Getting Started
Current PF Tek
Shoebox Assembly
All The Agar Info You Could Want
How It Should & Shouldn't Look


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Re: Penis envy isolation part 3 [Re: LtLurker]
    #25348105 -

i'd have to check. i wasn't as organized at the beginning. turns out you really need to label and document like a mofo when undertaking something like this.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25348111 -

I bet man. Couple dozen plates each transfer is a lot to keep track of.


--------------------
Space Ape Project
Links For Getting Started
Current PF Tek
Shoebox Assembly
All The Agar Info You Could Want
How It Should & Shouldn't Look


Edited by LtLurker (07/26/18 02:55 PM)

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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25349061 -

verum subsequentis said:
i'd have to check. i wasn't as organized at the beginning. turns out you really need to label and document like a mofo when undertaking something like this.


Exciting.

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Re: Penis envy isolation part 3 [Re: verum subsequentis]
    #25349092 -

verum subsequentis said:
updated op with

BEHOLD







it is time to shake that ass




Thats a whole lotta ass...


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.

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Re: Penis envy isolation part 3 [Re: BigSurMoon]
    #25349105 -

Thank you for the advice, I see petrie dish orders in my future.


--------------------
Ginge



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Re: Penis envy isolation part 3 [Re: Skipio]
    #25349121 -

:yourock:  :whatshesaid:

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Re: Penis envy isolation part 3 [Re: Letsgrowstones]
    #25349748 -

Is this part of the PE Challenge with Psycho Delicacy?
:nowaydude:


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