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Invisiblevinsue
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clone from dried caps
    #2390329 - 03/01/04 08:04 AM (12 years, 10 months ago)

Is it possible to start a clone on agar using mushroom sample that was dried and has been frozen for over 1 year? Original sample was extremely potent. Thanks


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OfflineFloyd_
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Re: clone from dried caps [Re: vinsue]
    #2390396 - 03/01/04 09:16 AM (12 years, 10 months ago)

unless its a species that can withstand freezing its most assuredly 100% dead. And even if it is a species that can withstand freezing..its still probably dead


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Anonymous

Re: clone from dried caps [Re: Floyd_]
    #2390667 - 03/01/04 11:37 AM (12 years, 10 months ago)

No clone but you can maybe get some spores to germinate.


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Offlineaje
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Re: clone from dried caps [Re: vinsue]
    #2406436 - 03/07/04 10:30 PM (12 years, 10 months ago)

I wouldn't bet on it, but if you have the resources to spare you may as well try it. You could get lucky.


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OfflineScourge
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Re: clone from dried caps [Re: ]
    #2407038 - 03/08/04 02:40 PM (12 years, 10 months ago)

i agree, spore germination would probably be your best bet.


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OfflineMitchnast
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Re: clone from dried caps [Re: Scourge]
    #2424848 - 03/12/04 06:19 PM (12 years, 10 months ago)

i bet if you tried to clone a dried cap, you would germinate the spores anyway, unless you used peroxide first


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OfflineRogerRabbitM
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Re: clone from dried caps [Re: vinsue]
    #2438551 - 03/16/04 10:47 AM (12 years, 10 months ago)

Dried caps have been known to start growing again on agar. Go for it.


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Anonymous

Re: clone from dried caps [Re: RogerRabbit]
    #2438564 - 03/16/04 10:55 AM (12 years, 10 months ago)

What!?!? Thats crazy are you just fucking around or serious. Well any way thats cool!


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OfflineCaptainFuture
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Re: clone from dried caps [Re: RogerRabbit]
    #2440093 - 03/16/04 05:53 PM (12 years, 10 months ago)

NEVER!
A dried cap is not a sklerotia...


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Offlinesnowspeed1
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Re: clone from dried caps [Re: vinsue]
    #2441863 - 03/17/04 03:06 AM (12 years, 10 months ago)

Actually i read a post on this board about a guy who did it with a 20 year old mushroom. there was little left but dust. never underestimate the power of a determined nature. he used peroxide on it as well as a safety measure.


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InvisibleJoshua
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Re: clone from dried caps [Re: snowspeed1]
    #2441891 - 03/17/04 03:19 AM (12 years, 10 months ago)

"unless its a species that can withstand freezing its most assuredly 100% dead."

This is true.

Cubensis fruit bodies do not survive dessication. I personally am not aware of any species whos fruitbody will survive dessication, especially for 20 years.

It is probable that what was grown from the cap were spores and not tissue.

Joshua


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OfflineRogerRabbitM
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Re: clone from dried caps [Re: vinsue]
    #2442524 - 03/17/04 10:24 AM (12 years, 10 months ago)

With all due respect to those who disagree or have never done it, you can take dried mycelium and get it growing again on agar. I've done this many times with dry mushrooms. The stipe works better than the cap. This is not due to spores germinating, but due to the dried mushroom tissue rehydrating and beginning to grow again.


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Offlineragadinks
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Re: clone from dried caps [Re: RogerRabbit]
    #2442632 - 03/17/04 10:57 AM (12 years, 10 months ago)

Does that depend on the species? With what species did you try that out?
Did you just put them on agar or did you rehydrate them first?


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OfflineNewdude1
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Re: clone from dried caps [Re: RogerRabbit]
    #2442642 - 03/17/04 10:59 AM (12 years, 10 months ago)

Seems to be some debate on this, I think maybe it calls for an experiment with pictures RodgerRabbit? mabye some dried myc, dried fruit, and dried cap spores, that would be really cool.


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OfflineShroomInhaler
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Re: clone from dried caps [Re: Newdude1]
    #2443106 - 03/17/04 01:43 PM (12 years, 10 months ago)

So what some of you are saying is that if i purshase dry mushrooms from someone, I don't need a spore syringe or anything, I can just try and use the dried msuhrooms??


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OfflinePooPs
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Re: clone from dried caps [Re: ShroomInhaler]
    #2443157 - 03/17/04 01:59 PM (12 years, 10 months ago)

I'll bet there were spores on the stem, causing germination of more mycelium..

But that's just my dumb ass opinion !


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InvisibleJoshua
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Re: clone from dried caps [Re: RogerRabbit]
    #2443463 - 03/17/04 03:16 PM (12 years, 10 months ago)

This is news to me! I'll try it and see what happens.

Joshua


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Anonymous

Re: clone from dried caps [Re: Joshua]
    #2443712 - 03/17/04 04:09 PM (12 years, 10 months ago)

Ok well though I am skeptic. I do believe that this could be true. Yeast a cusin of fungi, does reanimate when dried and rehydrated. So I guess this could be true for mushrooms. As long as the cell walls arnt damaged in the drying process. Hmmmm


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InvisibleJoshua
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Re: clone from dried caps [Re: ]
    #2447599 - 03/18/04 04:43 PM (12 years, 10 months ago)

Surely someone else has knowledge on the subject!

Joshua


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The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


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Offlinebobmarley4prez
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Re: clone from dried caps [Re: ]
    #2447614 - 03/18/04 04:48 PM (12 years, 10 months ago)

Yeast a cusin of fungi

yeast is a term used to describe a unicellular fungi of three of the four phyla of fungi (zygomycota, ascomycota, and basidiomycota(most mushrooms).
Peace


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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