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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

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Offlinemg420
journeyman
Registered: 02/09/04
Posts: 100
Last seen: 11 years, 7 months
Absolute raging failure.
    #2389781 - 03/01/04 12:21 AM (12 years, 9 months ago)

First 'real' grow is doing absolutely nothing. By 'real', i mean that everything was ludacrisly sterile and effort was put into it, this time. Mycelium solution was used, not spores. Honey-water tek was used and the solution was loaded with myc.

1 week later, no growth.

I need some info cleared up. The incubation stage... The FAQs and teks have some blatently contradictory information. Some strictly say that after innoculation things should be kept at 86F, and others strictly say 70F. What's correct?

I'm pretty sure that the failure is in the flour. Does non-organic absolutely guarantee failure of all jars all the time?


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InvisibleHanky
wiffle bat.
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Registered: 08/30/03
Posts: 56,993
Loc: Great Southern Land.
Re: Absolute raging failure. [Re: mg420]
    #2389790 - 03/01/04 12:24 AM (12 years, 9 months ago)

86f for colonisation..........70 to 75f for fruiting.
explain exactly how you perpare and sterilise your jars,also explain your inoculation methods.


--------------------
Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]




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Offlinemg420
journeyman
Registered: 02/09/04
Posts: 100
Last seen: 11 years, 7 months
Re: Absolute raging failure. [Re: Hanky]
    #2389818 - 03/01/04 12:36 AM (12 years, 9 months ago)

Most importantly I'd like to find out if the flour is the issue.


Substrate was measured exactly as described in the tek's. Whole wheat flour was used because BRF couldn't be found. Lids were puctured, taped and foiled. Jars were steamed at a rolling boil for 1:15. Jars were cooled on a counter, then the fridge, covered the whole time and removed from the fridge before turning 'cold'. Innoc came from honey-water jars full of myc. 0.5cc/hole, 4 holes/jar. Jars were not retaped, but foil was put back on.

Sterilization was non-stop. The entire work area was wiped down with alcohol, hair was covered, no breathing out of mouth. syringe was flame sterilized after every jar as well as before the whole process.

Jars were kept in a large rubbermade bin that contained a small bucket of water with a fishtank heater in it. The bin was covered so it retained heat and humidity. They were not looked at for 1 week because nobody was home.

There is no growth of anything. No myc and no visible contam. The jars still seem to have a great moisture content and the dry verm layer is still dry.


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InvisibleZapotecorum
AlcoHoliC

Registered: 01/15/04
Posts: 186
Loc: my conversion van
Re: Absolute raging failure. [Re: mg420]
    #2389819 - 03/01/04 12:36 AM (12 years, 9 months ago)

try keeping it simple, mmgg those those jars.


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Invisiblecheesenoonions
??????????????

Registered: 04/02/01
Posts: 584
Re: Absolute raging failure. [Re: mg420]
    #2389874 - 03/01/04 01:40 AM (12 years, 9 months ago)

maybe you have a bacterial contam. You can't always see those. Do they smell funky? If you can't get BRF, try getting some long grain brown rice and blending that down to a powder in a blender. Long grain brown rice or brown rice should be available at your grocery store in bags.


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InvisibleHanky
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Re: Absolute raging failure. [Re: cheesenoonions]
    #2389903 - 03/01/04 02:00 AM (12 years, 9 months ago)

yeah the wheat flower may be your problem,i've never heard of anyone using it before as mentioned above you can make your own BRF. or you can grind down millet,WBS etc.

best to start from scratch using a more sutible substrate,sucks i know.


--------------------
Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]




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Invisiblesimplemachine
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Registered: 09/14/03
Posts: 1,981
Re: Absolute raging failure. [Re: cheesenoonions]
    #2389904 - 03/01/04 02:01 AM (12 years, 9 months ago)

yeh try smelling the open holes...If it smells like rotten fruit it's bacteria.


SM


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Offlinemg420
journeyman
Registered: 02/09/04
Posts: 100
Last seen: 11 years, 7 months
Re: Absolute raging failure. [Re: mg420]
    #2390853 - 03/01/04 12:43 PM (12 years, 9 months ago)

I have read that whole wheat can be used in place of BRF, but is not as good of a choice. I'll post what it smells like.

What is MMGG? I've read this acronym hundreds of times, but i've never found a clear explination of it.


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Offlinemg420
journeyman
Registered: 02/09/04
Posts: 100
Last seen: 11 years, 7 months
Re: Absolute raging failure. [Re: mg420]
    #2390919 - 03/01/04 01:03 PM (12 years, 9 months ago)

The honey-water jars were the biggest success of the project.

new info: one of the jars has a dime-sized myc growing on it! it was not noticed before.

Jars have no odd smells... at least nothing pungeant.

honeywater had many many strings of myc, and a lot of them were stuck together in clumps. Is that what's supposed to happen? Jars were shaken before liquid was put in syringe and as the plunger was pulled it was aimed at myc to get as many as possible.

btw, this is B+ strain.


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Invisiblecheesenoonions
??????????????

Registered: 04/02/01
Posts: 584
Re: Absolute raging failure. [Re: mg420]
    #2391138 - 03/01/04 02:19 PM (12 years, 9 months ago)

a bit of advise with the honey water: Try putting something in there to break up the mycelium. I don't have any suggestions as to what to use, but I have heard people using pieces of glass and similar things. This way you break up the mycelium into small pieces. This does two things. It allows easier entry into the syringe through the needle. Sometimes the mycelium is really hard to suck into the syringe. The second thing is that it allos for many points of innoculation. each little piece of myc. will start to grow. This means faster colonization. good luck


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Offlinemg420
journeyman
Registered: 02/09/04
Posts: 100
Last seen: 11 years, 7 months
Re: Absolute raging failure. [Re: mg420]
    #2391200 - 03/01/04 02:35 PM (12 years, 9 months ago)

the myc was only tiny strings before they were sucked up. simply shaking broke up the clumps pretty well. Glass will be tried next time, but that's really not the problem right now. There are 11 jars with absolutely no growth and I don't know why.


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OfflineRoseM
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Registered: 09/24/03
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Re: Absolute raging failure. [Re: mg420]
    #2391229 - 03/01/04 02:45 PM (12 years, 9 months ago)

OK... without pictures, or being able to smell the jars... it is hard to say.

Here are some theories.

Perhaps you should wait another week.

Perhaps wheat wasn't the best substrate.

Perhaps your honey water was growing more than just myc.

Perhaps you didn't grow myc at all in your honey jars... many contams can be mistaken for myc.

Perhaps your jars were too wet/dry/hot/cold...

Perhaps the substrate contained some sort of a fungicide.

Perhaps you didn't get as much myc in your syringe/jars as you thought.

Are your jars getting enough air?

Did you use spores in your honey water jar? Perhaps you grew a slow growing B+ strain in your honey water.

I think it is either your honey water or your substrate... perhaps both.

More details or photos are needed to be more helpful... you are growing something white... that is a good sign.

Did you inoculate the sides of your jars or the middle?


--------------------
Fiddlesticks.



Edited by Rose (03/01/04 02:51 PM)


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OfflinePiano
Tay Inna Weeyan
Registered: 01/23/04
Posts: 43
Last seen: 8 years, 11 months
Re: Absolute raging failure. [Re: Rose]
    #2392140 - 03/01/04 08:04 PM (12 years, 9 months ago)

Just a thought from a newb--I used whole wheat in my PF jars, and it seems to be working fine for me.
Most are about 98% colonized, somewhere around 3 weeks.


--------------------
"Relax, stupid! Everything you see is make-believe." --Homer Simpson


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Invisibleblink
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Registered: 03/31/02
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Re: Absolute raging failure. [Re: Piano]
    #2392452 - 03/01/04 09:59 PM (12 years, 9 months ago)

Did you do multispore innoc or did you use the honey water tek?
mg was using the honey water tek. 1 week and no results, he is wondering if he should continue waiting because he has heard that colonization generally begins after only 3 days or so...

Is it impatience or is it legitimate concern?


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Invisibleblink
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Re: Absolute raging failure. [Re: blink]
    #2396332 - 03/02/04 08:03 PM (12 years, 9 months ago)

bump


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Invisiblesimplemachine
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Registered: 09/14/03
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Re: Absolute raging failure. [Re: blink]
    #2396384 - 03/02/04 08:22 PM (12 years, 9 months ago)

yeah blink if you honey/water innculated jars haven't shown any growth, it's probably infected with bacteria. Open one up and give it the sniff.


Liquid innoc sounds easy, but it takes some practice. You have to be VERY clean to avoid growing bacteria in an LC jar. Were you using a glovebox or flowhood? How much myc did you let grow in the LC jar before innoculation?


SM


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OfflineKaptKid
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Re: Absolute raging failure. [Re: mg420]
    #2396573 - 03/02/04 09:14 PM (12 years, 9 months ago)

ON the MMGG ver 3.2 say's to use 2/3 cup of verm.DON'T DO THIS.
Only use 1/2 cup as per the PF Tek.
Sounds like what I just went thur for two tryies.
Not enough water content.
Birhted my 1st cakes thurday.


--------------------
Child of the 60's, Tripping ever since.


:sun:


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

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