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OfflinePsyWonderCap
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Registered: 07/19/05
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3rd Failure! I Need HELP!
    #4574095 - 08/24/05 10:06 AM (11 years, 3 months ago)

Setup info:
Brf cakes in 1/2 pint jars
82-85 deg constant
Knocked up with Golden Teachers
Syringes from hawk's eye

So my first 2 sets of jars did nothing after 3 weeks but slowly one by one get contam'd. With the last of this syringe i started my 3rd set. They are doing as the others only one is contam'd and no growth on others...its been 2 weeks today. I am a cook so i know i measured my substrate right, the conditions are of correct temp (RH is like 35 but i didnt think that mattered at this point)My substrate is very fluffy and when i injected it spread all the way to the bottom. Sadly i think its a syringe problem. I shook the shit out of it, dont know why i cant get this to grow. EVEN a shitty grown, just SOMETHING! Help my guys/gals PLZ


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OfflineLoriath
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Re: 3rd Failure! I Need HELP! [Re: PsyWonderCap]
    #4574101 - 08/24/05 10:12 AM (11 years, 3 months ago)

describe your preparation procedures? like cleaning, and making the substrate. Did you PC your jars or just boil them in a big pot? did you flame the tip of your syringe before using? did you use an extra layer of verm to finish off the top of the jar to help fight contam? I am still a newb but after reading many many teks and posts those are the only things that came to mind. I have not yet grown any, still waiting on my spores. I'm sure someone can be of more help, just thought i'd try.


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OfflinePsyWonderCap
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Re: 3rd Failure! I Need HELP! [Re: Loriath]
    #4574114 - 08/24/05 10:24 AM (11 years, 3 months ago)

Thx man...to clarify

I was very clean and have a completly plastic'd closet shelf in a small room to which has a hepa runnin 24/7. I did boil but i boiled for 1 1/2 hours. I flamed the syringe and properly let cool (least 15 minutes) I alcohol whiped between each jar. I did use the dry verm layer on top after i had wiped the jar.


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InvisibleNewbie
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Re: 3rd Failure! I Need HELP! [Re: PsyWonderCap]
    #4574144 - 08/24/05 10:44 AM (11 years, 3 months ago)

I think 15 minutes is too long of a cooling time. The minute it was cool bacteria could have made it their home. Wipe the needle immediately with alcohol after you flame it, that way it will sterilize it MORE, and it will also cool it down. The minute that alcohol swab leaves the needle, inoculate the jar.


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OfflinePsyWonderCap
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Re: 3rd Failure! I Need HELP! [Re: Newbie]
    #4574166 - 08/24/05 10:52 AM (11 years, 3 months ago)

so in theory if i held the alcohol swap to the need and just slid it past into the jar...alcohol wouldnt hurt substrate if i didnt let the alcohol dry? this is good news. Still doesnt explain why i have no growth tho...sadly :-) Thanx tho NShroomie


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OfflineHoss
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Re: 3rd Failure! I Need HELP! [Re: PsyWonderCap]
    #4574234 - 08/24/05 11:09 AM (11 years, 3 months ago)

How long did you wait before you knocked up those jars after boiling them? Because the jars need to sit and cool off for a while. If you didn't wait long enough the inside of the jars could have still been hot, and the spores would have been killed.

If this is the case, then next time give your jars about 12 hours of cooling time before you knock them up.


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Invisiblesrgtm1a
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Re: 3rd Failure! I Need HELP! [Re: PsyWonderCap]
    #4574305 - 08/24/05 11:41 AM (11 years, 3 months ago)

well, your techniques sound like you are doing everything ok. boiling works, but isn't the best as far as getting rid of contams. PC is always the best method...you can pic one up pretty cheap.

The only thing I could see wrong is the 15min wait between flamming and knocking up your jar. As previously said, this could be where your problem was.

next time, swab the tip with rubbing alcohol, flame, then swab with alcohol again to cool the needle down, then inject right after that. Repeat that btwn jars. Quicker you get that needle sterilized and into the substrate, less chance of contam.

Bad syringes do happen, but not very often, So I don't know if this is a bad syringe....there are some holes in your sterilization techniques that could have caused this. 2 main problems I see are:

1. not using a PC.
2. 15min wait btwn flaming and inoculation.


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InvisibleNewbie
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Re: 3rd Failure! I Need HELP! [Re: PsyWonderCap]
    #4574310 - 08/24/05 11:45 AM (11 years, 3 months ago)

Quote:

PsyWonderCap said:
so in theory if i held the alcohol swap to the need and just slid it past into the jar...alcohol wouldnt hurt substrate if i didnt let the alcohol dry? this is good news. Still doesnt explain why i have no growth tho...sadly :-) Thanx tho NShroomie




I've heard of people placing an alcohol pad over the hole and sticking the need through it. The alcohol might kill some spores, but plenty will survive to germinate. I didn't even use alcohol with mine, I just flamed it red, then stuck it in after a couple seconds, then reflamed after each new jar. Good luck man!


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Offlinecoda
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Re: 3rd Failure! I Need HELP! [Re: Newbie]
    #4574772 - 08/24/05 02:37 PM (11 years, 3 months ago)

it could be the syringe, if you knocked up a bunch of jars and didnt see any growth, your syringe may be bunk. Contact the vendor and let them know, they'll prolly send you a replacement.


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To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG



Don't fuck with the laughing jesus.


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Offlinehyphae
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Re: 3rd Failure! I Need HELP! [Re: coda]
    #4575076 - 08/24/05 03:59 PM (11 years, 3 months ago)

I've seen some good questions asked here and some good advice. First mistake newbies make most often is not letting the jars cool enough they say well they were barely warm when they knocked up their jars, well warm means well over 106F. in the centers of the jars this temp will not kill spores but 122F. will so just keep this in mind. Shaking the syringe was smart, another newbie mistake (forgetting to). Heating the needle cherry it will be cool to the touch in about ten seconds, try it sometime with a used needle. The alcohol swab would be just added insurance. Tip: You do not have to flame the needle each and every time you dispense spores (fact). Only reflame after each batch of jars or if the needle contacts anything other than the lids holes, this tip will save you time and effort and will only fine tune your sterile culture technique. GL


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Getting the most out of your casings!, A pinning strategy.
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"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflinePsyWonderCap
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Re: 3rd Failure! I Need HELP! *DELETED* [Re: hyphae]
    #4576304 - 08/24/05 09:44 PM (11 years, 3 months ago)

Post deleted by PsyWonderCap

Reason for deletion: double post



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OfflinePsyWonderCap
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Registered: 07/19/05
Posts: 73
Last seen: 11 years, 2 months
Re: 3rd Failure! I Need HELP! [Re: PsyWonderCap]
    #4576315 - 08/24/05 09:49 PM (11 years, 3 months ago)

well i left them sitting over night in the pot i boiled them in ...soo i'm pretty sure they were cool, they felt cool. Just a note guys i was more directed towards why they didn't grow rather than why they got contam'd. I mean it took like a full 2 1/2 weeks for them to even start there contam. I was just sayin i was pissed off about no myc after 2 weeks! Lol but yea i was doing the needle thing wrong and i think i had my hepa blowing 2 close over the jars. BAD IDEA i guess :mad2:


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"Dunk and Roll" How-to


Edited by PsyWonderCap (08/24/05 09:57 PM)


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