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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: practical method for long term storage of mycelium in d [Re: Quincunx]
    #2329557 -

A method also used by many cultivators is to scrape the mycelium from the agar surface, add water, and suck it into a syringe.



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Re: practical method for long term storage of mycellium in d [Re: Quincunx]
    #2329643 -

Sorry Quincunx, I did not want to offend you by calling the lab of yours "fancy". Maybe the blue light ( fluorescent lamp ? ) and the ependorfer tubes made me think you would have a fancy lab.
But even if you had one it would be ok. I would be glad to have a fancy lab.

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Re: practical method for long term storage of mycelium in d [Re: Anno]
    #2333417 -

Quote:
A method also used by many cultivators is to scrape the mycelium from the agar surface, add water, and suck it into a syringe.


Couldn't some small amounts of agar nutrients be dissolved in water this way? If some agar particles get inside, how much of a problem is this? Maybe i'm too obsessed with separating nutrients from water and preserving but i guess it can't hurt.

ragadinks,
Hey, no offence taken. I'm sorry if i sounded like that. I just have a small spare room for mushroom cultivation. The blue light tone comes from UV desinfectant light. I turn it on when working with mycellium cultures.

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Re: practical method for long term storage of mycelium in d [Re: Quincunx]
    #2337486 -

>Couldn't some small amounts of agar nutrients be dissolved in water this way?
For sure.

> If some agar particles get inside, how much of a problem is this?
IME, not a problem.

> Maybe I'm too obsessed with separating nutrients from water and preserving but i guess it can't hurt.

I was also storing small colonized agar cubes in water, I didn?t notice any difference in terms of life expectancy and vigor.

My preferred method to store cultures up to 2 years is to store agar wedges in a small plastic baggie.
The only thing one has to be careful about is to never let the bagiies dry out, always keep them sealed in a tupperware in fridge.


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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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