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OfflineBruiser
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Registered: 04/17/02
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Storage
    #1410683 - 03/26/03 08:00 AM (15 years, 1 month ago)

Well, I lucked out today and got 8 dozen new Pyrex test tubes with screw caps for $40 on ebay. My question for everybody is this: What are people's experieces with storage using, (a) Agar Slants, and (b) Distilled sterile water? I was going to make a bunch of slants, but method (b) seems a little easier. Any experiences with either of these methods?
-Bruiser


Method (b) for those who aren't familiar with it:

From: "Perf. Fungi E."
Date: Thu, 2 Apr 1998 22:22:33 +1
Subject: Quotes about water storage
Below is a compilation of texts about the water storage
method, which I found today.
Oei, Peter. 1991. MANUAL ON MUSHROOM CULTIVATION - TECHNIQUES,
SPECIES AND OPPORTUNITIES FOR COMMERCIAL APPLICATIONS IN
DEVELOPING COUNTRIES. Transfer of Technology for Development
(Amsterdam) and Technical Center for Agricultural and Rural
co-operation (Wageningen), ISBN 90 70857 22 7, p 256:
"A simple technique is to grow the culture on an agar medium
and to keep small pieces of colonizad agar floating in
demineralized water. If 100 ml bottles are used, then these
should be fille with 75 ml demineralized water. Sterilize the
bottles for two hours, let them cool, then transfer
aseptically small pieces from the agar culture. Put about
three to four pieces of 0.5 x 0.5 square centimeters in each
bottle. Always inoculate at least three bottles per strain, so
some contamination occurs then there is still a back-up.
Strains can easily be recovered by taking a piece of the agar
out of the water and transferring it to a new slant. This
operation is not as messy as the mineral oil technique.
Strains can be kept for at least one year without losing
vigour (Exept for VOLVARIELLA VOLVACEA, which cannot stand
prolonged storage at temperatures below 12 centigrade)."


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OfflineRaadt
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Registered: 06/07/02
Posts: 2,104
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Re: Storage [Re: Bruiser]
    #1410815 - 03/26/03 08:48 AM (15 years, 1 month ago)

I use slants, and have had no problems with them.

I however have not tried the latter method.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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OfflineBruiser
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Re: Storage [Re: Raadt]
    #1410842 - 03/26/03 08:57 AM (15 years, 1 month ago)

Cool. Thanks Raadt.  :smile: I have plenty of tubes, so I'll make both. I was just looking for some feedback.  :laugh: I'm curious to know if anyone has tried the sterile water method for long term storage and how the results were.
-Bruiser 


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OfflineBruiser
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Re: Storage [Re: Bruiser]
    #1413094 - 03/27/03 04:24 AM (15 years, 1 month ago)

So I'm guessing nobody else has tried storage in sterile water?
-Bruiser


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