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Registered: 01/02/03
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storage of liquid mycellial cultures
    #1184092 - 01/02/03 05:55 AM (15 years, 5 months ago)

how long can liquid mycellial cultures, such as the result of spore germination in honey water or an established malt extract broth be stored in order to be used for future innoculating material, and under what conditions?

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Re: storage of liquid mycellial cultures [Re: gdr]
    #1184142 - 01/02/03 06:25 AM (15 years, 5 months ago)

how long is a pretty circumstantial question. most teks on honey water answer this question fairly well. Personally I inoculate as soon as the solution has cooled from sterilization. I allow it to incubate for 48 hours or more if necessary (watch for strings of mycelium to form) shaking daily. At this point you can further incubate for massive mycelial growth (not really necessary, unless you wanna eat those masses) or you can refrigerate for longer term storage. How long you could store it is hard to answer. It really depends on how fast your mycelium eats up the available nutrients. If you inoculated and then immediately refrigerated it would probaly have nutrients for over a month. A quick test is to take it out of refrigeration and incubate for ~32 hours and watch for growth. If it grows there are still nutes avail. Not really sure why you would need long term storage of solution, but that's just me personally, I make it when I need to use it and use it within a week.

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Re: storage of liquid mycellial cultures [Re: gdr]
    #1184978 - 01/02/03 12:06 PM (15 years, 5 months ago)

This might be of interest for you.

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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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