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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Puffball
    #1908986 - 09/12/03 11:12 AM (13 years, 5 months ago)

Now this is nothing more than an experiment.
From what I have read puffballs have never been successfully fruited in the lab.
I doubt I will have better luck but I have to at least try.
I found these growing in a lawn which was very abundant though they are very small as well.







I took a mature specimen and a wetted slide and puffed the spores on it.
This image is 40x microscope magnification photographed by a 3 mega pixel camera at full size.
I think this species is Bovista plumbea.


Click on image to enlarge.

I cloned it to agar and let it grow out.

Next I will try to expand the mycelium and try to fruit it.


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Offlinezeronio
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Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 5 months, 14 days
Re: Puffball [Re: MycoFactory]
    #1909076 - 09/12/03 11:51 AM (13 years, 5 months ago)

Very interesting.
I'd like to see how mycellium looks like.


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OfflineFungi_x
Original Shroomer
Male User Gallery
Folding@home Statistics
Registered: 08/17/03
Posts: 6,819
Loc: Michigan Flag
Last seen: 1 month, 27 days
Re: Puffball [Re: zeronio]
    #1909146 - 09/12/03 12:16 PM (13 years, 5 months ago)

Wow, Please Keep us updated :laugh:


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HAPPY FUCKING HOLIDAYS!


Edited by fungi_x (09/12/03 12:16 PM)


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Invisiblee7c
xyz
Registered: 04/29/03
Posts: 93
Re: Puffball [Re: Fungi_x]
    #1909202 - 09/12/03 12:35 PM (13 years, 5 months ago)

I remember stomping those as a kid, to see the satisfying green poof come out
Are they poisonous or edible or hallucinogenic?


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InvisibleCow Shit Collector
Patty Poacher

Registered: 02/15/01
Posts: 1,959
Loc: Random Field
Re: Puffball [Re: e7c]
    #1909949 - 09/12/03 04:34 PM (13 years, 5 months ago)


Try to get some spores from this species :wink:


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_______________________________________
CSC


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~Joe Dirt

Off Topic Website


Edited by Cow Shit Collector (09/12/03 04:35 PM)


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Offlinewalk
ignorantwhiteman

Registered: 06/22/03
Posts: 123
Loc: Dunedin, New Zealand
Last seen: 9 years, 9 months
Re: Puffball [Re: Cow Shit Collector]
    #1911894 - 09/13/03 05:20 AM (13 years, 5 months ago)

They are fuckin exelent to eat! Well the giant ones anyway.
Mainly cos they are huge and you can cut mushroom stakes. mmmmm big fat crumbed 'mushroom' stakes.
After we ran a mature one over with the car we took a wad of gleba and Zildjian tried spores on MEA to no avail.
What species are those MycoBag, are they edible or one of the poisonous?


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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Re: Puffball [Re: zeronio]
    #1913768 - 09/13/03 09:41 PM (13 years, 5 months ago)

Here is a photo of the mycelium on PDA.


A close up


The spores are brown in these small little puffballs. I think this species is Bovista plumbea. They are considered to be edible but they are so small it would take a lot of them for a meal. Perhaps as a snack, mushroom poppers :smile: 


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Offlinewalk
ignorantwhiteman

Registered: 06/22/03
Posts: 123
Loc: Dunedin, New Zealand
Last seen: 9 years, 9 months
Re: Puffball [Re: MycoFactory]
    #1914268 - 09/14/03 01:13 AM (13 years, 5 months ago)

Next time Im in my home town i will check my patch of giants and see if i can get us a culture.


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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Re: Puffball [Re: walk]
    #1917773 - 09/15/03 11:57 AM (13 years, 5 months ago)

Outside of pictures I have never seen these giants before. I imagine it would be wild to walk up on one or several :smile:


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Anonymous

Re: Puffball [Re: MycoFactory]
    #1917846 - 09/15/03 12:38 PM (13 years, 5 months ago)

thats great mycobag,
It will great to see how this turns out.:)

I saw my first giant puff ball this year.  I ran away.  I thought it was an alien pod or something. scared the shit out of me.

well, I finally realize that it was a mushroom once I looked in my identification book for the alien pod.  To this day I am still alarmed to have seen a mushrooms with the diameter of 15 inch.

e7c - I used to stomp on the little ones too when I was a kid. I am only sticking to the little ones. :smile:

peace

zerohero


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Offlinerommstein2001
Rise ye Must!
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Registered: 05/10/01
Posts: 3,180
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Re: Puffball [Re: ]
    #1919301 - 09/15/03 08:59 PM (13 years, 5 months ago)

The small ones are excellent batter dipped and fried! mmm


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InvisibleZildjian
human

Registered: 09/11/02
Posts: 208
Loc: new zealand
Re: Puffball [Re: walk]
    #1920864 - 09/16/03 10:03 AM (13 years, 5 months ago)

fuckin gleba!
Man, summer is going to be the best ever. I'll have to add "mushroom hunt with walk and peace_patrol" to my list of important things to do.


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Offlinewalk
ignorantwhiteman

Registered: 06/22/03
Posts: 123
Loc: Dunedin, New Zealand
Last seen: 9 years, 9 months
Re: Puffball [Re: Zildjian]
    #1922471 - 09/16/03 07:41 PM (13 years, 5 months ago)

Yeah man. So has no-one heard of anyone successfully cultivating puffballs?
I guess i will have to do some research. Me and Zildjian will hopefully get a culture of the giant puffball, in the next few months, and will distribute to those interested.
How goes your experimenting Mycobag?


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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Re: Puffball [Re: walk]
    #1924925 - 09/17/03 01:44 PM (13 years, 5 months ago)

It's going. It is not the fastest growing but then I may be a little on the cooler than optimum side of the temperature. I am going to bump it up a few degrees and see if that helps. Right now it is between 76 and 80 degrees. I am going to make a dozen more plates of this and then continue with substrates. I started with 3 plates and am down to one. The last one is the one pictured above. I had one plate develop a bacterial contam. I used another to do a liquid culture transfer to a dung/straw bag. There is bound to be a lag time to leap off to this new food source. Perhaps it will show something soon.
I will keep you updated.


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Offlineu4ia
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Registered: 01/23/03
Posts: 240
Last seen: 9 years, 11 months
Re: Puffball [Re: MycoFactory]
    #1924988 - 09/17/03 02:14 PM (13 years, 5 months ago)

Are these "puffballs" the same thing I used to jump all over as a kid?

They would release some dusty shit if you got them when they were ripe.

I've seen quite a few pictures on this site, they all have been white. I remember the ones I used to step on as being tan-ish, brown-ish, etc.

They were all different sizes, once in a while we'd find a big one, big enough that we would rip it off and throw it around the street for a few minutes.

Fun times.


--------------------
Procrastination is like masturbation, it's fun until you realize you are only screwing yourself.


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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Re: Puffball [Re: u4ia]
    #1925619 - 09/17/03 05:24 PM (13 years, 5 months ago)

I bet most of us as kids stomped all over these with joy as they puffed out their smoke  :grin:
These turn from white to brown as they get old. There is a picture in a previous post.

haha almost a puffball-hacky-sack game. I love it.


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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Re: Puffball [Re: MycoFactory]
    #1943951 - 09/23/03 12:41 PM (13 years, 5 months ago)

Well I made a special agar and then transferred mycelium to 20 new dishes.
You can see it is just now starting to leap off to the new food.
I am going to expose the dishes to different conditions to try to narrow down what it likes best.
Some of you may know this is suspected of being a mycorrhizal mushroom.



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Offlinewalk
ignorantwhiteman

Registered: 06/22/03
Posts: 123
Loc: Dunedin, New Zealand
Last seen: 9 years, 9 months
Re: Puffball [Re: MycoFactory]
    #1953409 - 09/26/03 05:32 AM (13 years, 4 months ago)


These are from the patch where we will hopefully get our culture.
Any news Mycobag?


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InvisibleMycoFactory
Mr MycoBag

Registered: 04/02/03
Posts: 549
Loc: NC, USA
Re: Puffball [Re: walk]
    #1953938 - 09/26/03 11:51 AM (13 years, 4 months ago)

I would so like to have a culture of a giant puffball :smile:

The mycelium seems to like the new agar. I have also found it prefers cooler temperatures with slight variable increases.
I now have the cultures sitting around 60 to 65 degrees with warming trends to near 70.
Believing this mushroom is mycorrizal I have introduced another new element to it and have found it to be interesting to say the least.
It is germinating rye grass! check out the picture!



You can see the mycelium following along the root tip reaching out in all directions.
It is growing at a greatly accelerated rate compared to cultures without the grass.

The first couple of times I tried this I have had contamination follow along with the seed.
We will have to see if I can keep it away this time.
Wish me luck!
Mycobag   


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Offlinewalk
ignorantwhiteman

Registered: 06/22/03
Posts: 123
Loc: Dunedin, New Zealand
Last seen: 9 years, 9 months
Re: Puffball [Re: MycoFactory]
    #1954715 - 09/26/03 04:41 PM (13 years, 4 months ago)

Damn, thats great.
Special agar?
You got me all worked up, im going to go do something.
Good luck.


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