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Shop:   Original Seeds Store Buy CBD, Compare CBD   Amazon ½ Pint Jars, Agar, Rye Grain, Vermiculite

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Invisiblerebelmoon
enthusiast
Registered: 04/05/00
Posts: 204
Re: loose lids on rye jars?
    #14987 - 04/30/00 02:05 PM (17 years, 29 days ago)

rye grai is very susceptible to contams in comparison to the pf jars w/ vermiculite toplayers. what i do is to use one piece plastic canning lids with a 1/2" hole cut in the middle. then either use filter discs underneath or use polyfil (see workmans site for details on polyfil). if you go with regular dome lids, i would suggest trying 9ers idea (find the tek in the grow section). i highly siggest getting plastic lids though. they dont rust and theyre all one piece. dont buy them form fungi perfecti. oreder from the ball/kerr canning hotline cuz they are the exact same and much cheaper. if oyu cant find the 800 number, and are interested, lemme know and ill get it for you.
-daniel.


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"habit is the ballast that chains the dog to its vomit" - s beckett


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Anonymous

Re: loose lids on rye jars?
    #14989 - 04/30/00 04:23 PM (17 years, 29 days ago)

thanks alot rebelmoon...I just have the lids loose now and when I loosened them (after about a week since dropping the agar in) they actually hissed like pressure had built up. Not sure if that is CO2 build up or pressure from temp (around 85)...either way, I have been spraying the surrounding area as well as the lids themselves with Lysol...some spreading is starting but it seems slow that's why I suspect it may be CO2 related...guess I'll wait and see...again, thanks


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Invisiblerebelmoon
enthusiast
Registered: 04/05/00
Posts: 204
Re: loose lids on rye jars?
    #14990 - 04/30/00 10:30 PM (17 years, 29 days ago)

after a week yu should have *at least* 50% colonization. i usually can get total colonization of a pint jar in 8 days. are you using a pure strain? it most like is that your water ratio is off. how did you prepare the grain?
the best way i have found is to cook it like you would cook rice. one full pint jar dry rye = three jars cooked. fill the jar 2/3 full. use an agar wedge or liquid innoculant. after innoculating shake each jar rigorously. after four days the whole jar should be pretty shite looking. you should shake and spread the mycelium. then after another four days do it again until there is little-no grain showing.
-daniel.


--------------------
"habit is the ballast that chains the dog to its vomit" - s beckett


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Invisiblerebelmoon
enthusiast
Registered: 04/05/00
Posts: 204
Re: loose lids on rye jars?
    #14991 - 04/30/00 10:31 PM (17 years, 29 days ago)

fucking quadruple post an shit...

[This message has been edited by rebelmoon (edited May 01, 2000).]



--------------------
"habit is the ballast that chains the dog to its vomit" - s beckett


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Invisiblerebelmoon
enthusiast
Registered: 04/05/00
Posts: 204
Re: loose lids on rye jars?
    #14992 - 04/30/00 10:44 PM (17 years, 29 days ago)

god...

[This message has been edited by rebelmoon (edited April 30, 2000).]



--------------------
"habit is the ballast that chains the dog to its vomit" - s beckett


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Invisiblerebelmoon
enthusiast
Registered: 04/05/00
Posts: 204
Re: loose lids on rye jars?
    #14993 - 04/30/00 10:47 PM (17 years, 29 days ago)

damnit!

[This message has been edited by rebelmoon (edited April 30, 2000).]



--------------------
"habit is the ballast that chains the dog to its vomit" - s beckett


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Anonymous

Re: loose lids on rye jars?
    #14994 - 04/30/00 11:46 PM (17 years, 29 days ago)

hehe...am I seeing double??
thanks again reb...
here's what I did.
My buddy gave me a recipe for doing the grain and it was 3/4 c water to 1 c rye...every recipe I've read here has the ratio exactly opposite..(100ml grain to 175ml water). Well I follwed his recipe cuz he already had colonizing jars and you can't argue with success. Mixed the water with the rye, put the lids on (almost tight)and cooked at 15 lbs. for about 45 min. When it cooled I checked and the grain had obviously soaked up all the water but the volume in the jar had barely increased so I figured there was not enough water. I added enough to adhere to the other ratio (1 cup grain to 1 3/4 cups water) and cooked it all again for closer to an hour...I think if anything that may be my problem. I heard that the grain is not supposed to crack. Doesn't make sense to me but whatever...I added 2 wedges of agar to each jar as you describe and shook vigorously...they have been sitting on a heating pad for over a week and there IS some develpoment but SLOOOWWW. Today I loosened the lids slightly and it hissed as though there was pressure so I think that the CO2 has inhibited growth somewhat.
So.....do you think it's CO2 or too much water? I'm afraid I don't know the strain beyond the fact that they are Psy Cubs..got the agar plate from my friend.


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Invisiblerebelmoon
enthusiast
Registered: 04/05/00
Posts: 204
Re: loose lids on rye jars?
    #14995 - 05/01/00 01:01 AM (17 years, 28 days ago)

could also be bacteria contamination. if oyu have a bunch of jars. open one. smell it. if oyu smell anything that isnt rye grain or a mushroom odor (especially sour) then it is probably contamed. also how hot was the heating pad. thermal detah is 106 deg f and you want no higher than mid 80s (84-86 is ideal). lower than that is ok but better if its in the 80s.
could be co2 but with that little amount of growth i cant see the gases being at a suffocating level. mycelium doesnt need oxygen, but it can get suffocated in its own gases (co2 etc).
but hey it might start to grow faster with the lids loosened. i say your best bet is to start over. maybe oyu should try transferring the mycelium to new agar plates. unless you used the whole thing. that could help as well. select the most rhizo growths on the plate and transfer to a few more plates. watch for the fastest growing sections and transfer again. then use that mycelium to innoculate new jars. cook the rye before hand and dont add free standing water. pressure cook for at least an hour. longer is better and with no freestanding water the kernels wont burst.
try and getting filter discs or some other means for gas exchange. my filter discs work alright but if i were to do it again id probably go with a cheaper method (polyfil).
lemme know how it turns out.
-daniel.


--------------------
"habit is the ballast that chains the dog to its vomit" - s beckett


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OfflinePanTrop
enthusiast
Registered: 11/03/99
Posts: 288
Loc: Washington
Last seen: 15 years, 3 months
Re: loose lids on rye jars?
    #14996 - 05/02/00 09:56 PM (17 years, 27 days ago)

If your Rye grain cracks its due to oversaturation. Why this is a problem is that once the jars are being colonized and you need to break apart the grain kernals if they have exploded there going to form a mess and cause problems when you try to break the rye up. Im not sure if you know this but Im going to say it anyway.....Soak the Rye for NO LESS than 24 hrs! and pressure cook them at 15psi for ATLEAST AN HOUR! I would go with 1.5 hours....makes me feel safer I guess? :biggrin:


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" My mind dont work if my spine dont jerk!"


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Invisiblephrozendata
Carpal Tunnel

Folding@home Statistics
Registered: 04/24/00
Posts: 5,015
Re: loose lids on rye jars?
    #14997 - 05/02/00 10:13 PM (17 years, 27 days ago)

I clicked this link so I could stop seeing it in red all the fucking time because I didnt visit it. And I posted to tell you that :cool:


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"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


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Anonymous

Re: loose lids on rye jars?
    #14998 - 05/02/00 10:21 PM (17 years, 27 days ago)

Would someone happen to know the 1 800 number from kerr? I am interested in using these plastic lids with my PF tech. I have had problems using metal lids which bend when you try to put a nail through the corners... If anyone has used these before please post and let me know about your success or ????
Thanks
Alaman


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