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truffle cultivation
    #14564030 -

http://en.wikipedia.org/wiki/Tuber_magnatum



data

$1500/kg

inoculated trees lasts 10 years to start production.

why not to start a colaborative effort for develop a better technique?

somebody have spores/mycelium?


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cultivando en la miseria SuctoSpore® Pictorial Tek

x7x_x7x@shroomery.org carl_jung_in_lsd@yahoo.com koh samui and oak ridge are my favourite strains

Edited by x7x_x7x (11/26/16 12:50 PM)

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Re: truffle cultivation [Re: x7x_x7x]
    #14566219 -

Who has truffle spores?


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This is a public computer, 1,000's of people use it everyday this isn't me typing this.  I dont even know how I got on this site, how the hell do I even work this computer.:wow: 


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Re: truffle cultivation [Re: ChronicSmoke]
    #14566910 -


http://www.ducketttruffieres.com/about_truffles.htm

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Re: truffle cultivation [Re: x7x_x7x]
    #14566917 -

http://the-plant-directory.co.uk/truffle-trees/summer-truffle-for-the-uk/truffle-tree-oak-sapling-with-summer-truffle-spores

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Re: truffle cultivation [Re: x7x_x7x]
    #14568451 -

Dude lets make some fucking money, I really like this idea.

If these trees are the key to truffles why are there not more truffle fields going up?

I mean if you find the right investors, put together a good presentation this cant lose.


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This is a public computer, 1,000's of people use it everyday this isn't me typing this.  I dont even know how I got on this site, how the hell do I even work this computer.:wow: 


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Re: truffle cultivation [Re: ChronicSmoke]
    #14568578 -

the key would be to grow the mushrooms without the tree, in an artificial susbtrate. truffles take 10 years in grow. :frown:

Edited by x7x_x7x (11/26/16 12:51 PM)

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Re: truffle cultivation [Re: x7x_x7x] * 1
    #14574009 -

PLus most trees that are inoculated dont produce truffles. in GGMM stamets writes that out of all the companies in the U.S. to try truffle production the only ones that are profitable are the ones selling the trees.

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Re: truffle cultivation [Re: deadmandave]
    #14574712 -

it is possible to make a living growing truffles but you need patience and cash for a long term investment some people have only gotten truffles after 12 years growing in New Zealand and places like that.

Read books by Ian Hall he has a fair amount of useful info in his books.

we are so far from understanding the process involved in truffle cultivation that i would guess that artificial cultivation is at least 20 years away.  I hope we never get it right so that some things are always a mystery :grin:


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One of these days all the answers will be revealed until then we learn from each other!

www.mushrush.co.za

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Re: truffle cultivation [Re: solumvita]
    #15506705 -

solumvita said:
it is possible to make a living growing truffles but you need patience and cash for a long term investment some people have only gotten truffles after 12 years growing in New Zealand and places like that.

Read books by Ian Hall he has a fair amount of useful info in his books.

we are so far from understanding the process involved in truffle cultivation that i would guess that artificial cultivation is at least 20 years away.  I hope we never get it right so that some things are always a mystery :grin:


No joke..I worked on an indoor truffle tree project for two years before giving up.  I applied hydroponics technology to tweek the grow techniques and had wonderful plants, but no fruiting bodies...it is a long and serious outdoor time/money investment.


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Re: truffle cultivation [Re: SporeFarmer]
    #15513424 -

Interesting sporefarmer, how were the truffles introduced to the plants and what type of plants were you growing?

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Re: truffle cultivation [Re: deadmandave]
    #15514156 -

hazelnut trees and rockrose bushes.  Hazelnuts tend to grow faster than oaks, so we chose them.  We inoculated using a variation of the 'lucky dip' method.  Tuber Mel. spores were extracted from fresh truffles by drying and warming the fruiting bodies in the oven...and then added to water and added to a recirculating dwc (deep water culture) hydroponics system.


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Re: truffle cultivation [Re: SporeFarmer]
    #15514510 -

Good ideas, though heating the truffles in the oven sounds dangerous.
Did you add lime to the substrate to raise PH?
Did you check the roots for signs of mycorrhiza, like short and fat parts?

Carsten

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Re: truffle cultivation [Re: SporeFarmer]
    #15514530 -

Is heating the truffles stimulating some sort of releasing mechanism?  Do they use brush fires in nature to facilitate this?

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Re: truffle cultivation [Re: Mycelio]
    #15514544 -

Mycelio said:
Good ideas, though heating the truffles in the oven sounds dangerous.
Did you add lime to the substrate to raise PH?
Did you check the roots for signs of mycorrhiza, like short and fat parts?

Carsten


Yes they produce hyphae (the fat, short, yellow nodule like growths that transform the white root structure).  No lime was added as there is no substrate.  They were grown in bubbling buckets of water, as a pure hydroponics grow, versus a soilless media such as coco fiber or alike.  If you are referring to growing spores on a substrate, truffles do not grow on nutrient rich media, and only form symbiotic relationships with certain species of plant/tree.  They are ectomycorrhizal symbioses with the roots of usually oak, hazelnut, or other similar tree species.  Heating the truffles was only to dry them.  I cut and pasted my old 'truffle tree notes' below.  Hopefully it will help someone one day.


Growing Conditions:

pH 7-8.5 with 7.9 being ideal
-----------------------------------
Necessary Nutrients:

macro elements:  N, P, K, Mg
- micro elements: B, CU, Mn, Zn
- C/N ratio
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Inoculation methods:

1.) Inoculation by contact with mycorrhizal roots

Root pieces from a "mother plant" can be placed in contact with the roots of a young seedling during potting. Alternatively, the entire "mother plant" can be planted in a container surrounded by rooted seedlings. About 2-3 months are required to produce mycorrhizal seedlings

2.) mycelial inoculation

A root tip covered in truffle fungus can be emulsified and sprayed onto the roots of a uninoculated tree in a hydroponic type system.

3.) inoculation by spores

The inoculation is usually performed under semi-sterile conditions and the substrate is generally soil that favors truffle growth. Spore suspensions are prepared from fresh, frozen or dried fruit bodies and are delivered to rooted seedlings or germinated seeds. This method requires 8-10 months to obtain well colonized plants.

Fruitbodies of T. melanosporum were sterilised and suspended in deionised water. 20 ml of spore suspension containing 5 106 spores/ ml were added to 800 grams of soil. Plant growth and mycorrhizal colonisation was assessed after 5-14 months of culture in a climate chamber.
The fruitbodies were then stratified in sand, at 4-5  C in the dark for 60 days, before the spore suspensions were prepared. The peridium was peeled off and the gleba was first homogenized with a blender and then ground in a mortar. When at least one third of the asci had been broken without damaging the spores water was added. Each seedling received 10 ml spore suspension. The number of spores ranged between 2 106-7 106 spores per seedling. After 3-7 months of growth in a greenhouse the root systems were examined for colonization of Tuber and other ectomycorrhizal species. The results showed that the degree of colonization increased with the time of incubation. It was also observed that the natural substrate gave a better colonisation than the artificial one, probably due to differences in pH and humidity.

After collection and identification the fruitbodies were cleaned and dried at 40 C for 48h. The approximate number of spores per gram of dried fruitbody was 107. The fruitbodies were chopped and mixed with tap water until the spores were released. Spore suspensions were prepared in spring and 10 ml were applied to each seedling at concentrations of 102, 103 and 104spores per seedling. Plants were grown in a controlled environment for 5 months

After some months the seeds germinate and the plants have 4-5 leaves; they are carefully extirpated; the taproot is blunted and the radical apparatus is immerged in a solution with truffle spores for two days. This solution is prepared by employing well-ripe truffles, which have rotten in distilled water for a few days until they become easy to pulp by hand. The small plants are placed in 1-2 litre vases employing suitable grounds. Subsequently, a cubic centimetre of  swill (full of truffle spores) is placed by the small plant neck. The small plants are watered during Summer and let grow in shaded zones until Autumn (when they are ready for the grounding). Well-drained, metalled, sub alkaline, organically-substance or mineral salts poor grounds are their favourites.


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Re: truffle cultivation [Re: SporeFarmer]
    #15514551 -





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Re: truffle cultivation [Re: SporeFarmer]
    #15514574 -

Is that colonized root tip from your plants?

Thanks for the notes :cool:

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Re: truffle cultivation [Re: SheikCorp]
    #15514680 -

The plants I had looked similar, yes, but I'm not 100% on that specific pic, as I pulled from my file from 3 years ago


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Re: truffle cultivation [Re: SporeFarmer]
    #15515816 -

Very interesting stuff!

Thank you for sharing,

JD


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Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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Re: truffle cultivation [Re: Javadog]
    #15516416 -

Javadog said:
Very interesting stuff!

Thank you for sharing,

JD


No problem...


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Re: truffle cultivation [Re: x7x_x7x]
    #23866319 -

i used to grow magic mushrooms with great success. i have now ordered truffles spores, agar jelly and petri dishes, my plan is as follows, put the spore on agar and wait for the mycelium to grow. then sterilize alkaline mud made alkaline by adding lime to it and then sterlizing it in a pressure cooker. Then plant a hazel, ash tree stump with roots in a container. then mix the mycelium with sterile water inject into the soil and roots, put it in a  fish tank indoors. Will this work? i cant find any info on the ineternet about growing truffles inside? let me know

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