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Tonearm
Stranger
Registered: 02/27/03
Posts: 184
Last seen: 19 years, 5 months
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Pressure cooking spawn bags 1
#1344565 - 03/01/03 07:09 AM (20 years, 10 months ago) |
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My buddy was pressure cooking a spawn bag with a filter patch on it for an hour. When he opened the cooker, the top was very difficult to get off and the bag had expanded to fill the entire interior of the cooker. It quickly cooled and went back to its normal size, but now it has all kinds of stretch marks on it. How do you keep it from expanding like that? Should the white cover of the filter patch be removed?
-------------------- My Favorite Link: http://www.deoxy.org/8brains.htm
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SixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
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Re: Pressure cooking spawn bags [Re: Tonearm]
#1344857 - 03/01/03 10:43 AM (20 years, 10 months ago) |
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Did ya seal it before you PC'ed it?
My neighbor simply loads, folds the tops over, PC's, allows to cool, does G2G, seals w/impluse sealer & incubates.
 6T (aka Mycota)
-------------------- ~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
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JssMthrFcknChrst
Son of the LordGod Almighty

Registered: 10/12/02
Posts: 446
Loc: Vatican City
Last seen: 19 years, 4 months
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Re: Pressure cooking spawn bags [Re: Tonearm]
#1346251 - 03/02/03 06:38 AM (20 years, 10 months ago) |
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My associate has found that if you squeeze the extra air out of a spawn bag before you seal it (however you want to do that) it won't expand as much in the PC. Also, the more bags in the PC at once, the less room there is for bag expansion. Keep a close eye on that spawn bag. The only bags that have contaminated for my associate have been the ones that streched, and he believes that the streching creates small openings, perfect for contamination.
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Tonearm
Stranger
Registered: 02/27/03
Posts: 184
Last seen: 19 years, 5 months
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Re: Pressure cooking spawn bags [Re: SixTango]
#1348197 - 03/03/03 04:30 AM (20 years, 10 months ago) |
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What's G2G? That's cool that it works to seal it after sterilization. How long would you PC for?
-------------------- My Favorite Link: http://www.deoxy.org/8brains.htm
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dragonfly
strange daze
Registered: 09/07/02
Posts: 86
Last seen: 20 years, 7 months
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Re: Pressure cooking spawn bags [Re: Tonearm]
#1348206 - 03/03/03 04:35 AM (20 years, 10 months ago) |
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g2g = grain to grain [transfer]
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majik_monkey
Always Learning
Registered: 01/24/03
Posts: 62
Loc: machu picchu
Last seen: 18 years, 1 month
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Re: Pressure cooking spawn bags [Re: Tonearm]
#1348223 - 03/03/03 04:41 AM (20 years, 10 months ago) |
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PC time varies on the amount your sterilizing. I do 60 min for 4-5 quarts in an 8 quart PC for 1 hour. Some people would do less, some more. I think less is pushing it and more is good if you have more time. Some lessons I've learned that might help: make sure there is proper amount of water in the PC. How do you know? for 4 quarts grain, use ~1+ quart water. Also, the towel that you put at the bottom of the PC should be fairly thick and made of cotton only. I've had towels burn through as a combination result of thin towel and water running out. I think people with electric stoves run into more of a problem then gas stove users...I've just noticed a tendancy for things in burn easier on electric. My 2c, hope it helps.
-------------------- There are more important things in life than just being really, really, ridiculously good looking. -Zoolander
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majik_monkey
Always Learning
Registered: 01/24/03
Posts: 62
Loc: machu picchu
Last seen: 18 years, 1 month
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Re: Pressure cooking spawn bags [Re: Tonearm]
#1348225 - 03/03/03 04:43 AM (20 years, 10 months ago) |
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PC time varies on the amount your sterilizing. My Uncle does 60 min for 4-5 quarts in an 8 quart PC for 1 hour. Some people would do less, some more. He thinks less is pushing it and more is good if you have more time. Some lessons He's learned that might help: make sure there is proper amount of water in the PC. How do you know? for 4 quarts grain, use ~1+ quart water. Also, the towel that you put at the bottom of the PC should be fairly thick and made of cotton only. I've had towels burn through as a combination result of thin towel and water running out. I think people with electric stoves run into more of a problem then gas stove users...I've just noticed a tendancy for things in burn easier on electric. My Uncle's 2c, hope it helps.
-------------------- There are more important things in life than just being really, really, ridiculously good looking. -Zoolander
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SixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
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Re: Pressure cooking spawn bags [Re: Tonearm]
#1348226 - 03/03/03 04:43 AM (20 years, 10 months ago) |
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6T's Grain to Grain Transfers (G2G)
Once you have learned to prepare sterilized spawn pint or quart jars of bird seed, rye or grains & fully colonize them with mycelium. You can easily propagate a single colonized quart jar of that into about 20 more via G2G transfers. Jars propagated via G2G transfers generally colonize under optimal conditions 100% in 10 to 14 days . Given that, spores do not have to germinate, as what is transferred to freshly prepared jars is active mycelium on fully colonized seed or grains.
The method is to prepare fresh jars, just as you would to inoculate via a spore syringe (soak seed, rinse, drain, load, apply filter disk & PC). Excepting, rather than inoculate the fresh jars with a syringe. You transfer grain from a colonized jar to fresh uncolonized jars. The procedure is simple & only requires common sense, minimal preparation, a long stout clean stainless steel spoon & the cleanest personal hygiene and the smallest uncarpeted working place you can muster.
Prepare the smallest cleanest uncarpeted room you have (generally, a bathroom). In the following manner. Clean the room as best you can, getting rid of any dirt, dust, mold or mildew. Remove any cloth hanging anywhere. Spray Lysol on everything, everywhere & wipe it down. If you have any hepa type air filter unit? Place it in the room & run it for at least 1 hour. Running a hepa is preferable, but, if you don’t have one. You can usually manage without it
Wipe your fully colonized jar of grain & your fresh jars down, with a clean Lysol sprayed rag. Place those in the room, on the counter top, or whatever flat working surface you intend to use. Wear freshly laundered clean cloths. If you have a face mask (preferable) wear it. If you have a shower cap to cover your hair (preferable), wear it. Enter the room, spray Lysol around (again) run the hepa for a few minutes (if you have one). Then, turn it off. Spray your hands & arms with Lysol & wipe dry.
Unscrew the lid off the colonized jar. Leave the internal filter disk or filter material in place covering the content. Unscrew the lid on a fresh jar, leaving the internal filter material in place. Remove the filter material from the colonized jar & dig up about ? of content, as it will be colonized into a solid mass. Spoon out 2 table spoons full & transfer them to the uncolonized jar, by lifting it’s filter up & spooning them in. Replace the filter material on the fresh jar IMMEDIATELY after spooning in the colonized material.
Repeat this same process as many times as you have fresh jars to transfer to. Once done. Screw the fresh jars lids on tight. Cover the outside of the lids of the fresh jars with a double layer of alcohol swabbed coffee filters & rubber band them down. Shake each fresh jar to spread the colonized material throughout it. Place your fresh jars in a dry, dark, warm place (preferably between 78 & 84 F), and allow them to colonize in peace & quiet. G2G transfer & shaking jars batters the mycelium. It takes it a day or 3 recuperate from that shock. There is no need to shake G2G jars more than once. As, doing so will only slow colonization, rather than speed it up.
-------------------- ~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~
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