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Newbie WBS and PCing [update: success! pics added]
    #13292453 -

I was able to borrow an All American Model 915 pressure cooker from a family friend.  After thoroughly reading the instruction manual as well as doc34's WBS tek I went ahead with the procedure.

The lids I went with for now (until I make some fancy ones) are simple polyfill lids.  I drilled a 1/4" hole and pulled a wad of polyfill through.  I filled my quart jars to 1/2 full with prepped WBS as per doc34's tek, added a pinch of gypsum, and then shook the jars using a solid lid.  Then I removed the solid lid, wiped the inner rim of the jar mouth clean of WBS, and put on the polyfill lid.  Then I put two layers of aluminum foil over the jars and put them in the PC.  After PCing at 15psi for 60 mins I let everything cool overnight.

My questions are:

1) Does the WBS look too wet?  To me it looks too wet but of course this is my first attempt so no experience to compare against.  I let it drain for 20 minutes before loading it into the jars.  I think I maybe should have let it drain for longer or put it on some paper towels, I'll definitely do that next time.  These pics are after PCing, I took them out of the PC this morning about 45 mins before taking the pics.



2) I've read that shaking the jars with a filter lid is not good for the filter, as it will get the filter wet and promote contam growth.  Is it okay to shake the jars with the polyfill filter before knocking them up?  Or should I wait until 30% colonization before first shake?

The reason I'm asking is I only have 7cc of spore solution left before I'll be making my own spore syringes, and I'd prefer to use a known good source of spores with my first WBS attempt to minimize potential contams.  I have plenty of WBS, if my prep was off then I'd gladly re-do everything rather than waste good spore solution.  The next time I think I will follow The Feel Family WBS tek as it seems more thorough.

Also I did search and read before posting, but I wasn't able to determine whether or not my WBS is too wet based on the numerous threads I read on the subject.  Some posts I read seem to indicate my jars would be fine, but other posts make me think that I should re-do them.


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Cubensis-friendly LED lighting! LED Super Ribbon & LED Terrarium Tek

Edited by Ekyldog (10/14/10 04:08 PM)

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Re: Newbie WBS and PCing questions [Re: Ekyldog]
    #13292536 -

Dont shake them until they are 40% colonized, and even then its not nessesary. If your moisture content is off(over) then shaking it could tear apart the grains and adding more wet to the already too wet, thus upping your chances for contams, etc.

They look on point.maybe a tad wet. They look like mine that i did last night, i guess we'll BOTH know in a little while! lol


--------------------
"Life is like a shit sandwich...the more bread you have, the less shit you taste."          John Lennon

Dont listen to me...
I  give the worst advice!
All I Post is fictional,and the pictures are from the internet..( I know a guy who knows a guy..that knows a guy

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Re: Newbie WBS and PCing questions [Re: jakethebarber]
    #13295844 -

I decided to shake the jars since the WBS had formed into a large solid clump after PCing.  It took a lot of vigorous shaking to free all the WBS, but afterwards it was nice and loose in each jar.  The polyfill doesn't seem any worse for wear, as the WBS seems to have soaked up the excess water that was first showing after taking the jars out of the PC.

Today I knocked up the jars using GT spore solution.  I did not shake the jars immediately after inoculating as I've read it's better not to.  After the jars are showing about 30-40% colonization I'll give them a light shake to distribute the colonized kernels.  Then I'll give them one more shake just before I spawn them to my mini MSG.

I think I made the right choice by breaking up the WBS before inoculating.  It only makes sense that loose WBS will colonize better and faster than a solid block of WBS.  Worst case scenario I guess I'll have to start over, but I can always order more spore solution if I have issues making my own from prints.


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Cubensis-friendly LED lighting! LED Super Ribbon & LED Terrarium Tek

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Re: Newbie WBS and PCing questions [Re: Ekyldog]
    #13295877 -

Sounds like your text book spot on to me. ..yeah i found that shaking them while their still hot ..just enough to break them apart, is perfect. I do it again gently after they have cooled in my oven. I have a self-healing injector port in one hole, with a syringe cut in half and filled with poly-fil..then siliconed in the 5/8" hole, those are my lids..they look beautiful. I need to get a camera..im like the only one here who doesnt have one..wait i do!! and old security camera for my front door.


--------------------
"Life is like a shit sandwich...the more bread you have, the less shit you taste."          John Lennon

Dont listen to me...
I  give the worst advice!
All I Post is fictional,and the pictures are from the internet..( I know a guy who knows a guy..that knows a guy

Extras: Filter Print Post Top
Re: Newbie WBS and PCing questions [Re: Ekyldog]
    #13296037 -

nice, I am using MonsterMitchs tech atm (first go, first batch PC'ing as I type) http://www.shroomery.org/forums/showflat.php/Number/5963507#Post5963507%C2%A0]http://www.shroomery.org/forums/showflat.php/Number/5963507#Post5963507%C2%A0

And then for smaller batches I have used foomans. ( has worked really well)
http://www.shroomery.org/forums/showflat.php/Number/8508523#Post8508523]http://www.shroomery.org/forums/showflat.php/Number/8508523#Post8508523


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//Searching... Reading... Learning...

Supplies Canada
Damion5050's Links

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Re: Newbie WBS and PCing questions [Re: tusca]
    #13296095 -

Yeah i like Foomans' alot. i just checked my qrt wbs jars and they have maybe 2% busted grains!! Perfect!


--------------------
"Life is like a shit sandwich...the more bread you have, the less shit you taste."          John Lennon

Dont listen to me...
I  give the worst advice!
All I Post is fictional,and the pictures are from the internet..( I know a guy who knows a guy..that knows a guy

Extras: Filter Print Post Top
Re: Newbie WBS and PCing questions [Re: jakethebarber]
    #13296104 -

Nice ! :smile:

Also forgot this link this is like the bible.

Damion5050 links
http://www.shroomery.org/forums/showflat.php/Number/11961453


--------------------
//Searching... Reading... Learning...

Supplies Canada
Damion5050's Links

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Re: Newbie WBS and PCing questions [Re: tusca]
    #13296115 -

Definitley.. iv'e never seen that before...thats awesome..what a shortcut!


--------------------
"Life is like a shit sandwich...the more bread you have, the less shit you taste."          John Lennon

Dont listen to me...
I  give the worst advice!
All I Post is fictional,and the pictures are from the internet..( I know a guy who knows a guy..that knows a guy

Extras: Filter Print Post Top
Success so far! :) [Re: jakethebarber]
    #13336088 -

Thanks for all the helpful links guys, some excellent information there.

Just wanted to post a bit of an update.  So far my first WBS and rye grass seed jars seem to be doing fine (knock on wood). Here is a bit of a timeline:

10/5/10 - After the jars had cooled for about 24 hours, I sanitized my still air box with 70% IPA and the room air using Oust.  The 6 WBS jars and 1 RGS jar were inoculated through the polyfill using 1cc of vendor-supplied Golden Teacher spore solution per jar.  After inoculation the jars were placed on the top shelf in a closet to await colonization.

10/8/10 - Prepared another 5 jars of RGS, this time using 1 1/2 cups grass seed and 3/4 cup coffee/water mix.  The gypsum was added to the coffee/water mix before adding the RGS and shaking the jars.  This time I let the jars sit for about 4 hours before PCing them instead of 24 hours like last time.  The consensus seems to be that a few hours soak is sufficient with RGS and with 24 hours I noticed a bit of sprouting despite using coffee and gypsum.

Today I made my first home made spore syringe courtesy of a fresh PF Classic fruit that came out of my SGFC yesterday.  I followed RR's Spore Syringe video and all went well -- even made my own inoculating loop out of a piece of wire and a toothpick covered in electrical tape for the handle (gotta love ghetto tek).  I was able to make two 10ml syringes and two 3ml syringes.

10/9/10 - First signs of germination on the WBS jars and first RGS jar.  Yay! :smile:

In the afternoon I inoculated the other 5 jars of RGS after allowing 24 hours to cool.  I used my first home made spore syringe as described above.  This means I'll have 6 jars of WBS-GT spawn, 1 jar of RGS-GT spawn, and 5 jars of RGS-PF spawn.  Really looking forward to spawning to substrate when the time comes. :laugh:

10/12/10 - In the evening the RGS-PF jars showed their first signs of germination!  A few of the WBS-GT jars are showing signs of rhizomorphic growth. :smile:

10/14/10 - I decided to take some pictures today to document the progress of the jars.  I should have taken some pics earlier but hey, better late than never!

Here's the progress so far, this is day 9 for the WBS-GT and RGS-GT jars and day 6 for the RGS-PF jars:



RGS (GT) Colonizing - Day 9


RGS (PF Classic) Colonizing - Day 6


RGS (PF Classic) Colonizing - Day 6


WBS (GT) Colonizing - Day 9


WBS (GT) Colonizing - Day 9


WBS (GT) Colonizing - Day 9


WBS (GT) Colonizing - Day 9


WBS (GT) Colonizing - Day 9


To me the WBS jars look like they're getting close to the point where I can shake them, maybe in another day or two.  Overall I am very happy with my first grains attempt, again (knock on wood) here's to hoping it all turns out well in the end.

Comments/feedback is always appreciated. :smile:


--------------------


Cubensis-friendly LED lighting! LED Super Ribbon & LED Terrarium Tek

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