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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Petri Dish Work. [Re: Epilson Lyrae]
    #11391689 -

I would pay the extra money for the glass ones personally..  Also some of the plastic ones come with snap on lids they are not on the front page.  They come with white press on caps.

Well I am getting ready to do the agar work right now I will post some pictures when I am done.


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Re: Petri Dish Work. [Re: Damion5050]
    #11391830 -

All done.  Did 3 of each OR BPK and 3 of my GT isolate.



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Re: Petri Dish Work. [Re: Damion5050]
    #11392026 -

cool man.. I did my GT from a print.. the first two dishes looked like some kind of thick fuzzy contam.. but you could see it emanating from the spores by looking underneath the dish..so I kept transferring it.. my last transfer has lost all that fuzz, and just pure thick rhizo strands.. I have found a lot of interest in agar work :smile:

I also took some of that good GT rhizo and some good PE growth and threw them in the same dish.. lets see if they do it.


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Re: Petri Dish Work. [Re: Herbal_Elixer]
    #11392587 -

nice work guys. And sorry about the missundestading on the wedge size.


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:mushroom2:THOSE WHO KNOW DONT TALK!:mad:THOSE WHO DONT KNOW CANT TALK.:smile: (Timothy Leary):mushroom2:
"Funny thing about weekends when your unemployed they don't mean quit so much except you get hang out with your workin' friends" Les Claypool

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Re: Petri Dish Work. [Re: jerrylove]
    #11392613 -

jerrylove said:
nice work guys. And sorry about the missundestading on the wedge size.



Rookie mistake lol..  :whack:  Pay attention next time lol.. Just fucking with ya.


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AMU Q&A:plot:
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Re: Petri Dish Work. [Re: Damion5050]
    #11392634 -

haha ok man I'll try to pull my head out my ass lol


--------------------
:mushroom2:THOSE WHO KNOW DONT TALK!:mad:THOSE WHO DONT KNOW CANT TALK.:smile: (Timothy Leary):mushroom2:
"Funny thing about weekends when your unemployed they don't mean quit so much except you get hang out with your workin' friends" Les Claypool

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Re: Petri Dish Work. [Re: jerrylove]
    #11392656 -

jerrylove said:
haha ok man I'll try to pull my head out my ass lol



LoL I am surprised they dont have some sort of smiley face pulling his head out of someones ass. LoL


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Re: Petri Dish Work. [Re: Damion5050]
    #11392674 -

how big of a wedge do you guys use?


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By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

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Re: Petri Dish Work. [Re: SillyBilly]
    #11392693 -

Small.. I cut out about the size of a ball point pen tip.


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Re: Petri Dish Work. [Re: SillyBilly]
    #11392766 -

i guess my pieces are too big then to be isolates
probably should watch lets grow mushrooms again
coincidentally i did find some dentistry tools lying around that i was thinking about using


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

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Re: Petri Dish Work. [Re: SillyBilly]
    #11393287 -

I need to get some better tools.  Cause I am using a modeling scalpel and the fucking thing starts to flake off when it gets red hot.  And it leaves little steel flakes in my petri dishes lol.

You don't really need a big size chunk..  But then again you can use a big one if you want to..


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AMU Q&A:plot:
AMU Genetic Library:stirthepot:
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My Elementary Liquid Culture (LC) Tek
Educational Cultivation Link Collection


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Re: Petri Dish Work. [Re: Damion5050]
    #11393321 -

ok because i have been using like 3mm long 1mm wide pieces or 2x2's
i looked just now to see if i had an extra scalpel but i only have 2 extra blades and not an entire scalpel


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

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Re: Petri Dish Work. [Re: SillyBilly]
    #11393332 -

Like I said I normally use a piece about the size of the tip of a ball point pen..  But a bigger piece isn't really gona hurt anything.


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AMU Q&A:plot:
AMU Genetic Library:stirthepot:
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Educational Cultivation Link Collection


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Re: Petri Dish Work. [Re: Damion5050]
    #11393440 -

Damion5050 said:
I need to get some better tools.  Cause I am using a modeling scalpel and the fucking thing starts to flake off when it gets red hot.  And it leaves little steel flakes in my petri dishes lol.



I had the same problem and scalpels take long to cool down.
I now use old heavy gauge(18g) hypodermic needles for agar work.
If you flatten the tip of the needle correctly with a pliers then you will have great agar tools, remove the plunger from an old syringe then glue the needle to the syringe body, now you will have a handy cutting tool for agar and it will stand upright on its own while it cools down.
You can sharpen the flattened needle tip with some sandpaper if need be.
Hope it helps.


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Procrastination is like masturbation, when you look at the job in hand you are actually screwing yourself.

Edited by Getafixx (11/05/09 07:36 PM)

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Re: Petri Dish Work. [Re: Getafixx]
    #11393524 -

Good work on the transfers D...
Also check this site out for lab supplies,  it is my favorite but they change there stock weekly..

http://www.sciplus.com/

CH


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:mypoorbrain:

Questions about Cluster Headaches?  click here...
Additives info click here...

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Re: Petri Dish Work. [Re: CH HELL]
    #11393576 -

thanks for that link CH
how cool is this thing http://www.sciplus.com/singleItem.cfm/terms/14883


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

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Re: Petri Dish Work. [Re: SillyBilly]
    #11393617 -



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Procrastination is like masturbation, when you look at the job in hand you are actually screwing yourself.

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Re: Petri Dish Work. [Re: SillyBilly]
    #11393622 -

SillyBilly said:
thanks for that link CH
how cool is this thing http://www.sciplus.com/singleItem.cfm/terms/14883


Yeah bro keep looking they have cool shit,  If you have a cheap microscope they have things to make it better.  They have a usb adapter so you can view with your computer or take pictures.  Its pretty sweat.
CH


--------------------
:mypoorbrain:

Questions about Cluster Headaches?  click here...
Additives info click here...

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Re: Petri Dish Work. [Re: Getafixx]
    #11393713 -

Getafixx said:




cool idea


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Re: Petri Dish Work. [Re: Herbal_Elixer]
    #11393801 -

Thanks Herbal, I use a slightly thinner needle and bent/rolled it around a pen and makes for a good inoculation loop too.
It May look like shit but it serves a purpose and works well.


--------------------
Procrastination is like masturbation, when you look at the job in hand you are actually screwing yourself.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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