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Reuse plastic Petri dishes.
    #10986804 -

I have searched "how to reuse plastic Petri dishes" and I found a

couple of links that basically say it's very hard unless you have UV

light. But UV light is something that I am not interested in messing

around with. Is there any kind of bleach, alchohol, or peroxide

solution I can sterilize them with. If so how do I do it. Next time I


buy Petri dishes they will be glass.


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OM MADNE PADME OBIWAN LUKE HUM....

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Re: Reuse plastic Petri dishes. [Re: billycorgan55]
    #10987727 -

What's the problem with UV light, if I may ask? Is it because of eye-sight hazards?

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Re: Reuse plastic Petri dishes. [Re: amuzakat]
    #10989635 -

UV won't help much either, and glass petri dishes suck.  I donated mine to the local high school science department years ago.  Order a box of 500 disposable petri dishes and you'll be miles ahead.  You can't wash and re-sterilize the glass ones for the dime the plastic ones cost.  In addition, when you get into strain isolation, you will easily have over a hundred dishes going at any given time.  That gets awfully expensive with glass.  Furthermore, alcohol soaked gloves handling heavy glass dishes was causing me to drop and break at least one dish nearly every time I worked.  I hated the glass.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Reuse plastic Petri dishes. [Re: RogerRabbit]
    #10991243 -

Well i guess ill try to get more plastic dishes. Is there another kind of petri dishes that I can buy and reuse?


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OM MADNE PADME OBIWAN LUKE HUM....

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Re: Reuse plastic Petri dishes. [Re: billycorgan55]
    #10992414 -

Sorry plastic is your best bet like Doctor Rabbit says better just stick to the disposables.  It is just not worth it.


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One of these days all the answers will be revealed until then we learn from each other!

www.mushrush.co.za

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Re: Reuse plastic Petri dishes. [Re: solumvita]
    #10999921 -

I will agree with plastic being better than glass even though I use glass, only because i can reuse then time after time.

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Re: Reuse plastic Petri dishes. [Re: badman]
    #11002326 -

Just Me and I have been using quarter pint jars as petris for agar work.  They are far from ideal due to the difficulty in seeing what is going on in the jar and the height of the walls for making transfers...  On the note of cost in cleaning the glass ones, we've just been washing out the jars in the sink then mixing up the agar, and pouring it into the jars before pressure cooking in the jar.  They come out ready to go...


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[url=http:

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Re: Reuse plastic Petri dishes. [Re: SuchSmartMonkeys]
    #11004050 -

im looking at some on line but there different sizes whats a good size to get


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Self healing lids
noobies lids

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Re: Reuse plastic Petri dishes. [Re: cashus]
    #11004342 -

Diameter of 9-10 cm is standard

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Re: Reuse plastic Petri dishes. [Re: badman]
    #11024008 -

i've tried PCing them but they melted at 1 hour at 15 psi.

I bleached like 10 of them over 3 days. then washed with soap and water.

I will wrap all in aluminum foil and boil them for 20 minutes. take package out and put under GB.

I will let you know how it goes.


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Re: Reuse plastic Petri dishes. [Re: badman] * 1
    #11024196 -

> im looking at some on line but there different sizes whats a good size to get

Here's the best source I've found...
http://www.cynmar.com/SearchByCategory.aspx?CategoryCode=331

Get the 95-100mm size, using little ones sucks.

If you want to reuse plastic petri dishes UV probably won't cut it.  UV won't penetrate plastic so you'd have to lay them all open in a flow hood.  Even then it wouldn't work very well IMHO.

You can sterilize them in a bleach solution if you really want to.  It's a PITA though. You pretty much need a flow hood to dry them off in.  If you've got one of those I doubt you'd be asking about saving on petri dishes though.


Quote:
I autoclave plastic petri's every day at my lab with no melting but like I said it might be the quality as I have heard of others having melting issues.


Then either your autoclave isn't hitting proper temps or you're using the more expensive (and very rare) polymethylpentene (NALGENE) petri dishes.  They cost $6-8 EACH DISH, so I kind of doubt you're using them.

There's also dishes made out of PP, but nobody uses them because they aren't clear.  What kind of ghetto lab autoclaves plastic petri dishes anyways? 


-FF

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Re: Reuse plastic Petri dishes. [Re: fastfred] * 1
    #11025157 -

im looking into buying polypropolyne (sp) petri dishes. they are a little more expensive then the lame polystyrene ones we all use. on ebay i was finding them for quite good prices.

these can be autoclaved and reused. i dont know how they compare to glass but im guessing they dont break half as easy, arnt as heavy, are much cheaper and all around better.

i hate seeing people throw away petri dishes. i get all of mine from my university. when people fill at least one 55 gal autoclave bag a day in my lab alone with used dishes i have to take some. i clean em with soap and water. then long bleach soak, 1 hour or so 1/3 strength. then i wash off and then clean with a paper towel soaked in 100% iso alcohol. then i spray each one with a bunch of 100% iso (99% i guess) and put them inside down on a sterile surface to dry somewhat. then i pour my hot agar in.

i get a lot of condensation but im ok with it. i incubate my dishes upside down, aka the agar filled section up. so condensation doesnt get in the way of viewing etc. i usually get a few small bacterial growths after several days. but as long as i use forceps to remove some mcy instead of cutting out the agar, i have no problems.


uv lights do work. i tried the one in the lab the other day. in a (glove box or) flow hood, clean the plates first and then seperate the two halfs and lay in the light with the inside exposed for some time (10 minutes makes me more than comfortable). then i close them up right there and store them for later use.

the whole idea is to save some money, and to stop wasting so many dishes. if my school would just spend a little more money on pp petri dishes they wouldnt have to buy a new huge ass box every week for our small lab alone.

anyone have experience with the pp petri dishes? i dont :smile:


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Good places to start:
WBS TEK
Grain LC TEK
Spawn Bag TEK
Mini Mono Tub TEK
Sclerotia Mega-Thread

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Re: Reuse plastic Petri dishes. [Re: LitCloset] * 1
    #11025171 -

I've only seen them a couple times, but they aren't clear.  They are translucent.  Not all that cloudy, but not nearly as good as PS or glass.


-FF

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Re: Reuse plastic Petri dishes. [Re: fastfred]
    #11025273 -

ouch not very clear? that sucks...

how about polycarbonate? any one ever see petri dishes made of that? they have a melting temp of about 270C i think. they should be ok in a autoclave.

polycarbonate is beautiful. clear er than glass


--------------------
Good places to start:
WBS TEK
Grain LC TEK
Spawn Bag TEK
Mini Mono Tub TEK
Sclerotia Mega-Thread

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Re: Reuse plastic Petri dishes. [Re: LitCloset]
    #11025543 -

LitCloset said:
uv lights do work. i tried the one in the lab the other day. in a (glove box or) flow hood, clean the plates first and then seperate the two halfs and lay in the light with the inside exposed for some time (10 minutes makes me more than comfortable). then i close them up right there and store them for later use.


Can I ask what kind of UV-light you use? how many watts, and how close to the lamp do you place them?
I have a small UV-c light wand, but I guess it might not have enough juice for this task.

Anyway, thanks for the hints to you and everybody else. I've begun my ventures into agar so far just to be able to get a clean culture from unsterile prints, so I don't need the large number of petri dishes that are necessary for systematic isolation of substrains. That's why I'm looking into reusing petri dishes, because to take advantage of bulk discounts I'd have to buy more petri dishes than I could use in 5-10 years. :-)

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Re: Reuse plastic Petri dishes. [Re: amuzakat]
    #11026344 -

i dont have the lamp in front of me at the moment so details, sorry dont have em. i can say its 2 long bulbs in one lamp. they are both about 1 foot long. its a heavy duty lamp, we use it in the lab for mutations etc. usually 60sec is enough to kill off whatever organism im working with.

i tried a 1/2 pint jar last night with agar in the bottom. just pour, then pressure cook the entire thing. im leaving it out for a few days before using it to see how well the sterilization worked out. a lot of people complain this doesnt work but i bet one of the 1/4 pint wide mouths would work great.


--------------------
Good places to start:
WBS TEK
Grain LC TEK
Spawn Bag TEK
Mini Mono Tub TEK
Sclerotia Mega-Thread

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Re: Reuse plastic Petri dishes. [Re: LitCloset]
    #11027524 -

Thanks!

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Re: Reuse plastic Petri dishes. [Re: amuzakat]
    #11293812 -

Here is a source for the Nalgene autoclavable petri dishes.

    http://www.coleparmer.ca/catalog/product_index.asp?cls=42866

Not cheap, but reusable

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Re: Reuse plastic Petri dishes. [Re: M8M]
    #11302408 -

UV-c is the sanitizing light you'd use and like Fast Fred I use Cynmar for my petri dishes. They usually break a few in transit but 2-3 per 300 or 500 isn't that bad IMO.

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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