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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

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OfflineAzmodeus
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Plastic Petris'...am i screwed?
    #1288174 - 02/07/03 10:32 AM (14 years, 23 days ago)

I've been looking up info on agar cus i got some petri and agar last night.  But i just read that the plastic will melt in the PC...i NEED to pc it though... i think....
am i screwed? :confused:


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Lest we forget. "


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OfflineAnnoA
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288197 - 02/07/03 10:44 AM (14 years, 23 days ago)

>i NEED to pc it though

Why?


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InvisibleRoadkill
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288199 - 02/07/03 10:44 AM (14 years, 23 days ago)

plastic petri dishes usually come in a plastic sleeve(bag) that has be radiated to make it sterile.

if your petri dishes didn't come in this bag...or the bag is torn...there is a chance they aren't sterile anymore.

Why would you have to pc them?


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleG a n j a
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288205 - 02/07/03 10:46 AM (14 years, 23 days ago)

if your cloning with added peroxide you dont need to p.c IMO
but if your going to start spores you can microwave sterilise them in short burst(short bursts are important because you dont what the agar to bubble up and stick to the lid)

Edit lol forget what i said and listen to them i was microwaving mine lol


--------------------
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Edited by G a n j a (02/07/03 10:47 AM)


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Invisiblezeta
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288209 - 02/07/03 10:47 AM (14 years, 23 days ago)

Are you sure they aren't already sterile? I get sterile plastic petris sealed in sleeves of 20. But if they aren't sealed then they are useless because, as you said, you can't sterilise them.


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OfflineAzmodeus
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Re: Plastic Petris'...am i screwed? [Re: zeta]
    #1288228 - 02/07/03 10:57 AM (14 years, 23 days ago)

Thanks for the quick responces yall!

It came in a plastic sleeve bag, but it was open on top. Could i not just thouroughly wipe with isypropyl 70%? Thats what i use to sterilize syringes and tools , and containers....

Would i need to sterilize my agar mixture still?


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OfflineRaadt
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288249 - 02/07/03 11:07 AM (14 years, 23 days ago)

You always need to sterilize the agar.

I have glass petris, so I sterilize in the PC then wipe down with rubbing alcohol prior to pouring, if you wipe down, then pour your agar while still hot. It should be fine.


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OfflineAzmodeus
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Re: Plastic Petris'...am i screwed? [Re: Raadt]
    #1288253 - 02/07/03 11:11 AM (14 years, 23 days ago)

Hmmm, okay, im glad to hear i can re-use my plastic dishes...
i guess i'd sterilise my agar in a pint jar then. Any advice on keeping itfrom bubbling over? i heard just letting it cool naturally will sufice.


--------------------
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Lest we forget. "


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Offlinecanid
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288259 - 02/07/03 11:13 AM (14 years, 23 days ago)

yes, you'll have to sterilize your agar mixes. be careful that the vessel you PC your agar in will hold about three times the total volume of agar mix and water you are PCing if you have never PCd agar before in case it bubbles over.


--------------------



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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: Azmodeus]
    #1288260 - 02/07/03 11:13 AM (14 years, 23 days ago)



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InvisibleRoadkill
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Re: Plastic Petris'...am i screwed? [Re: vatoloco]
    #1288278 - 02/07/03 11:19 AM (14 years, 23 days ago)

Quote:

I had plastic petris that were pc safe.



What did you do with those?


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineAzmodeus
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Re: Plastic Petris'...am i screwed? [Re: vatoloco]
    #1288279 - 02/07/03 11:19 AM (14 years, 23 days ago)

Actually, i got em through a trade... dont know the manufacturer....ill see if i can find a brand name or sumthin.


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


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OfflineAzmodeus
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Re: Plastic Petris'...am i screwed? [Re: canid]
    #1288283 - 02/07/03 11:21 AM (14 years, 23 days ago)

Dont all the agar recipies make about a litre of stuff? good for around 30 petri dishes? i dont think i have a container wich will hold three times that volume and still fit.

What causes the bubbling? its rapid temp change right? would letting it cool at room temp cause it to boil over?


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


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OfflineAnnoA
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Re: Plastic Petris'...am i screwed? [Re: Roadkill]
    #1288305 - 02/07/03 11:27 AM (14 years, 23 days ago)

>I had plastic petris that were pc safe.
--------------------------------------------------------------------------------
>What did you do with those?

There are reusable plactic petris available, made of polypropylene, fully autoclavable.

For instance http://www.mycosupply.com/lab%20supplies/index2.html .


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InvisibleRoadkill
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Re: Plastic Petris'...am i screwed? [Re: Anno]
    #1288312 - 02/07/03 11:29 AM (14 years, 23 days ago)

Anno,

I think Vato traded them to me.

lolzz



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineRaadt
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288320 - 02/07/03 11:31 AM (14 years, 23 days ago)

half the recipe and make it in a quart jar, that's what i do. 500ml of water 10g agar add your nutes.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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InvisibleRoadkill
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Re: Plastic Petris'...am i screwed? [Re: Raadt]
    #1288329 - 02/07/03 11:34 AM (14 years, 23 days ago)

Quote:

half the recipe and make it in a quart jar, that's what i do. 500ml of water 10g agar add your nutes.


Me too



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineAnnoA
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288354 - 02/07/03 11:43 AM (14 years, 23 days ago)

>Dont all the agar recipies make about a litre of stuff? good for around 30 petri dishes?
1 liter ->40 dishes

> i dont think i have a container wich will hold three times that volume and still fit.
2, 3 containers?

>What causes the bubbling? its rapid temp change right?
Fast pressure loss at cooling down.



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Anonymous

Re: Plastic Petris'...am i screwed? [Re: Roadkill]
    #1288371 - 02/07/03 11:47 AM (14 years, 23 days ago)

I have been using those Grolsh Beer bottles somebody once talked about.
The first time I used it I closed the lid on all the way.  When I took it out and opened it ,The agar shot out like a damn cannon all over my kitchen.  I now
keep the lid open and just cover it with foil.  I still have some left over in the bottle could I still use it.  Its still clean but it has solidified.  Would just heating it like a baby bottle work? :smirk:


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OfflineAzmodeus
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Re: Plastic Petris'...am i screwed? [Re: Roadkill]
    #1288457 - 02/07/03 12:17 PM (14 years, 23 days ago)

My Pc fits quarts on thier side only....i thnk i'd have to do a pint or two!
(i made the reply about half an hour ago and just posted...)


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


Edited by Azmodeus (02/07/03 12:20 PM)


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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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