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 Azmodeus
Seeker
Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 20 years, 24 days
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 Plastic Petris'...am i screwed? 
#1288174 - 02/07/03 08:32 AM (21 years, 11 months ago) |
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I've been looking up info on agar cus i got some petri and agar last night. But i just read that the plastic will melt in the PC...i NEED to pc it though... i think....
am i screwed? 
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"Know your Body - Know your Mind - Know your Substance - Know your Source.
Lest we forget. "
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 14 days, 12 minutes
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
#1288197 - 02/07/03 08:44 AM (21 years, 11 months ago) |
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>i NEED to pc it though
Why?
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 Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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 Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
#1288199 - 02/07/03 08:44 AM (21 years, 11 months ago) |
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plastic petri dishes usually come in a plastic sleeve(bag) that has be radiated to make it sterile.
if your petri dishes didn't come in this bag...or the bag is torn...there is a chance they aren't sterile anymore.
Why would you have to pc them?
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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G a n j a
Pictish and proud
Registered: 12/03/02
Posts: 7,860
Loc: Zone ate
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
#1288205 - 02/07/03 08:46 AM (21 years, 11 months ago) |
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if your cloning with added peroxide you dont need to p.c IMO
but if your going to start spores you can microwave sterilise them in short burst(short bursts are important because you dont what the agar to bubble up and stick to the lid)
Edit lol forget what i said and listen to them i was microwaving mine lol
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er
Edited by G a n j a (02/07/03 08:47 AM)
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zeta
Stranger
Registered: 05/24/02
Posts: 3,972
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
#1288209 - 02/07/03 08:47 AM (21 years, 11 months ago) |
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Are you sure they aren't already sterile? I get sterile plastic petris sealed in sleeves of 20. But if they aren't sealed then they are useless because, as you said, you can't sterilise them.
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Azmodeus
Seeker
Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 20 years, 24 days
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Re: Plastic Petris'...am i screwed? [Re: zeta]
#1288228 - 02/07/03 08:57 AM (21 years, 11 months ago) |
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Thanks for the quick responces yall!
It came in a plastic sleeve bag, but it was open on top. Could i not just thouroughly wipe with isypropyl 70%? Thats what i use to sterilize syringes and tools , and containers....
Would i need to sterilize my agar mixture still?
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"Know your Body - Know your Mind - Know your Substance - Know your Source.
Lest we forget. "
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Raadt
nicht
Registered: 06/07/02
Posts: 2,107
Loc: azurescending
Last seen: 5 years, 8 months
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
#1288249 - 02/07/03 09:07 AM (21 years, 11 months ago) |
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You always need to sterilize the agar.
I have glass petris, so I sterilize in the PC then wipe down with rubbing alcohol prior to pouring, if you wipe down, then pour your agar while still hot. It should be fine.
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Raadt
-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--
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Azmodeus
Seeker
Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 20 years, 24 days
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Re: Plastic Petris'...am i screwed? [Re: Raadt]
#1288253 - 02/07/03 09:11 AM (21 years, 11 months ago) |
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Hmmm, okay, im glad to hear i can re-use my plastic dishes...
i guess i'd sterilise my agar in a pint jar then. Any advice on keeping itfrom bubbling over? i heard just letting it cool naturally will sufice.
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"Know your Body - Know your Mind - Know your Substance - Know your Source.
Lest we forget. "
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canid
irregular meat sprocket
Registered: 02/26/02
Posts: 11,946
Loc: looking for zeebras, n. c...
Last seen: 6 days, 21 hours
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
#1288259 - 02/07/03 09:13 AM (21 years, 11 months ago) |
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yes, you'll have to sterilize your agar mixes. be careful that the vessel you PC your agar in will hold about three times the total volume of agar mix and water you are PCing if you have never PCd agar before in case it bubbles over.
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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.
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vatoloco
Puppet Hunter -DBK
Registered: 01/30/02
Posts: 7,653
Last seen: 20 years, 4 months
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Post deleted by MOE THE MAD SCIENTIST [Re: Azmodeus]
#1288260 - 02/07/03 09:13 AM (21 years, 11 months ago) |
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Plastic Petris'...am i screwed? [Re: vatoloco]
#1288278 - 02/07/03 09:19 AM (21 years, 11 months ago) |
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Quote:
I had plastic petris that were pc safe.
What did you do with those?
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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Azmodeus
Seeker
Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 20 years, 24 days
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Re: Plastic Petris'...am i screwed? [Re: vatoloco]
#1288279 - 02/07/03 09:19 AM (21 years, 11 months ago) |
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Actually, i got em through a trade... dont know the manufacturer....ill see if i can find a brand name or sumthin.
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"Know your Body - Know your Mind - Know your Substance - Know your Source.
Lest we forget. "
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Azmodeus
Seeker
Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 20 years, 24 days
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Re: Plastic Petris'...am i screwed? [Re: canid]
#1288283 - 02/07/03 09:21 AM (21 years, 11 months ago) |
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Dont all the agar recipies make about a litre of stuff? good for around 30 petri dishes? i dont think i have a container wich will hold three times that volume and still fit.
What causes the bubbling? its rapid temp change right? would letting it cool at room temp cause it to boil over?
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"Know your Body - Know your Mind - Know your Substance - Know your Source.
Lest we forget. "
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 14 days, 12 minutes
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Re: Plastic Petris'...am i screwed? [Re: Roadkill]
#1288305 - 02/07/03 09:27 AM (21 years, 11 months ago) |
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>I had plastic petris that were pc safe.
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>What did you do with those?
There are reusable plactic petris available, made of polypropylene, fully autoclavable.
For instance http://www.mycosupply.com/lab%20supplies/index2.html .
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Plastic Petris'...am i screwed? [Re: Anno]
#1288312 - 02/07/03 09:29 AM (21 years, 11 months ago) |
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Anno,
I think Vato traded them to me.
lolzz
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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Raadt
nicht
Registered: 06/07/02
Posts: 2,107
Loc: azurescending
Last seen: 5 years, 8 months
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
#1288320 - 02/07/03 09:31 AM (21 years, 11 months ago) |
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half the recipe and make it in a quart jar, that's what i do. 500ml of water 10g agar add your nutes.
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Raadt
-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Plastic Petris'...am i screwed? [Re: Raadt]
#1288329 - 02/07/03 09:34 AM (21 years, 11 months ago) |
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Quote:
half the recipe and make it in a quart jar, that's what i do. 500ml of water 10g agar add your nutes.
Me too
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 14 days, 12 minutes
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Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
#1288354 - 02/07/03 09:43 AM (21 years, 11 months ago) |
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>Dont all the agar recipies make about a litre of stuff? good for around 30 petri dishes?
1 liter ->40 dishes
> i dont think i have a container wich will hold three times that volume and still fit.
2, 3 containers?
>What causes the bubbling? its rapid temp change right?
Fast pressure loss at cooling down.
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Anonymous
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Re: Plastic Petris'...am i screwed? [Re: Roadkill]
#1288371 - 02/07/03 09:47 AM (21 years, 11 months ago) |
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I have been using those Grolsh Beer bottles somebody once talked about.
The first time I used it I closed the lid on all the way. When I took it out and opened it ,The agar shot out like a damn cannon all over my kitchen. I now
keep the lid open and just cover it with foil. I still have some left over in the bottle could I still use it. Its still clean but it has solidified. Would just heating it like a baby bottle work? 
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Azmodeus
Seeker
Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 20 years, 24 days
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Re: Plastic Petris'...am i screwed? [Re: Roadkill]
#1288457 - 02/07/03 10:17 AM (21 years, 11 months ago) |
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My Pc fits quarts on thier side only....i thnk i'd have to do a pint or two!
(i made the reply about half an hour ago and just posted...)
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"Know your Body - Know your Mind - Know your Substance - Know your Source.
Lest we forget. "
Edited by Azmodeus (02/07/03 10:20 AM)
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