Home | Community | Message Board


Mushrooms.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

Jump to first unread post. Pages: 1 | 2 | Next >  [ show all ]
OfflineAzmodeus
Seeker

Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 11 years, 10 months
Plastic Petris'...am i screwed?
    #1288174 - 02/07/03 10:32 AM (13 years, 9 months ago)

I've been looking up info on agar cus i got some petri and agar last night.  But i just read that the plastic will melt in the PC...i NEED to pc it though... i think....
am i screwed? :confused:


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 12 days
Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288197 - 02/07/03 10:44 AM (13 years, 9 months ago)

>i NEED to pc it though

Why?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288199 - 02/07/03 10:44 AM (13 years, 9 months ago)

plastic petri dishes usually come in a plastic sleeve(bag) that has be radiated to make it sterile.

if your petri dishes didn't come in this bag...or the bag is torn...there is a chance they aren't sterile anymore.

Why would you have to pc them?


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleG a n j a
Pictish and proud
 User Gallery

Registered: 12/03/02
Posts: 7,860
Loc: Zone ate
Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288205 - 02/07/03 10:46 AM (13 years, 9 months ago)

if your cloning with added peroxide you dont need to p.c IMO
but if your going to start spores you can microwave sterilise them in short burst(short bursts are important because you dont what the agar to bubble up and stick to the lid)

Edit lol forget what i said and listen to them i was microwaving mine lol


--------------------
er


Edited by G a n j a (02/07/03 10:47 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblezeta
Stranger

Registered: 05/25/02
Posts: 3,972
Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288209 - 02/07/03 10:47 AM (13 years, 9 months ago)

Are you sure they aren't already sterile? I get sterile plastic petris sealed in sleeves of 20. But if they aren't sealed then they are useless because, as you said, you can't sterilise them.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzmodeus
Seeker

Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 11 years, 10 months
Re: Plastic Petris'...am i screwed? [Re: zeta]
    #1288228 - 02/07/03 10:57 AM (13 years, 9 months ago)

Thanks for the quick responces yall!

It came in a plastic sleeve bag, but it was open on top. Could i not just thouroughly wipe with isypropyl 70%? Thats what i use to sterilize syringes and tools , and containers....

Would i need to sterilize my agar mixture still?


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRaadt
nicht

Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 8 years, 10 months
Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288249 - 02/07/03 11:07 AM (13 years, 9 months ago)

You always need to sterilize the agar.

I have glass petris, so I sterilize in the PC then wipe down with rubbing alcohol prior to pouring, if you wipe down, then pour your agar while still hot. It should be fine.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzmodeus
Seeker

Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 11 years, 10 months
Re: Plastic Petris'...am i screwed? [Re: Raadt]
    #1288253 - 02/07/03 11:11 AM (13 years, 9 months ago)

Hmmm, okay, im glad to hear i can re-use my plastic dishes...
i guess i'd sterilise my agar in a pint jar then. Any advice on keeping itfrom bubbling over? i heard just letting it cool naturally will sufice.


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecanid
irregular meat sprocket
Male User Gallery

Folding@home Statistics
Registered: 02/26/02
Posts: 11,792
Loc: looking for zeebras, n. c...
Last seen: 1 day, 13 hours
Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288259 - 02/07/03 11:13 AM (13 years, 9 months ago)

yes, you'll have to sterilize your agar mixes. be careful that the vessel you PC your agar in will hold about three times the total volume of agar mix and water you are PCing if you have never PCd agar before in case it bubbles over.


--------------------



Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinevatoloco
Puppet Hunter -DBK
Registered: 01/30/02
Posts: 7,653
Last seen: 12 years, 2 months
Post deleted by MOE THE MAD SCIENTIST [Re: Azmodeus]
    #1288260 - 02/07/03 11:13 AM (13 years, 9 months ago)



Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Plastic Petris'...am i screwed? [Re: vatoloco]
    #1288278 - 02/07/03 11:19 AM (13 years, 9 months ago)

Quote:

I had plastic petris that were pc safe.



What did you do with those?


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzmodeus
Seeker

Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 11 years, 10 months
Re: Plastic Petris'...am i screwed? [Re: vatoloco]
    #1288279 - 02/07/03 11:19 AM (13 years, 9 months ago)

Actually, i got em through a trade... dont know the manufacturer....ill see if i can find a brand name or sumthin.


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzmodeus
Seeker

Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 11 years, 10 months
Re: Plastic Petris'...am i screwed? [Re: canid]
    #1288283 - 02/07/03 11:21 AM (13 years, 9 months ago)

Dont all the agar recipies make about a litre of stuff? good for around 30 petri dishes? i dont think i have a container wich will hold three times that volume and still fit.

What causes the bubbling? its rapid temp change right? would letting it cool at room temp cause it to boil over?


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 12 days
Re: Plastic Petris'...am i screwed? [Re: Roadkill]
    #1288305 - 02/07/03 11:27 AM (13 years, 9 months ago)

>I had plastic petris that were pc safe.
--------------------------------------------------------------------------------
>What did you do with those?

There are reusable plactic petris available, made of polypropylene, fully autoclavable.

For instance http://www.mycosupply.com/lab%20supplies/index2.html .


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Plastic Petris'...am i screwed? [Re: Anno]
    #1288312 - 02/07/03 11:29 AM (13 years, 9 months ago)

Anno,

I think Vato traded them to me.

lolzz



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRaadt
nicht

Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 8 years, 10 months
Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288320 - 02/07/03 11:31 AM (13 years, 9 months ago)

half the recipe and make it in a quart jar, that's what i do. 500ml of water 10g agar add your nutes.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Plastic Petris'...am i screwed? [Re: Raadt]
    #1288329 - 02/07/03 11:34 AM (13 years, 9 months ago)

Quote:

half the recipe and make it in a quart jar, that's what i do. 500ml of water 10g agar add your nutes.


Me too



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 12 days
Re: Plastic Petris'...am i screwed? [Re: Azmodeus]
    #1288354 - 02/07/03 11:43 AM (13 years, 9 months ago)

>Dont all the agar recipies make about a litre of stuff? good for around 30 petri dishes?
1 liter ->40 dishes

> i dont think i have a container wich will hold three times that volume and still fit.
2, 3 containers?

>What causes the bubbling? its rapid temp change right?
Fast pressure loss at cooling down.



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Plastic Petris'...am i screwed? [Re: Roadkill]
    #1288371 - 02/07/03 11:47 AM (13 years, 9 months ago)

I have been using those Grolsh Beer bottles somebody once talked about.
The first time I used it I closed the lid on all the way.  When I took it out and opened it ,The agar shot out like a damn cannon all over my kitchen.  I now
keep the lid open and just cover it with foil.  I still have some left over in the bottle could I still use it.  Its still clean but it has solidified.  Would just heating it like a baby bottle work? :smirk:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzmodeus
Seeker

Registered: 11/27/02
Posts: 3,392
Loc: Lotus Land!! B.C.
Last seen: 11 years, 10 months
Re: Plastic Petris'...am i screwed? [Re: Roadkill]
    #1288457 - 02/07/03 12:17 PM (13 years, 9 months ago)

My Pc fits quarts on thier side only....i thnk i'd have to do a pint or two!
(i made the reply about half an hour ago and just posted...)


--------------------
"Know your Body - Know your Mind - Know your Substance - Know your Source.

Lest we forget. "


Edited by Azmodeus (02/07/03 12:20 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2 | Next >  [ show all ]

Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Petri Process liveby 840 7 09/25/08 08:34 AM
by denger
* Petri dish question German Kahuna 1,430 14 01/10/09 06:33 AM
by German Kahuna
* Malt Agar Recipes NDK 3,425 7 09/06/01 02:25 PM
by blackout
* Pyrex petri dishes imperfect rims? Shaw 726 1 07/14/04 02:58 PM
by Anno
* Few Noob Questions About Using Petri Dishes And Agar glenbelt 4,510 14 02/03/09 12:13 AM
by The Shape
* Petris .... how much?
( 1 2 all )
MeneerCactus 1,747 25 03/23/03 08:22 AM
by Raadt
* Reusing plastic dishes YidakiMan 1,054 9 10/07/03 09:58 AM
by Champion des Champignons
* Req: Petri Dish Pics With Following Content...
( 1 2 all )
Sillypcybin 6,441 25 11/17/05 11:03 AM
by FooMan

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
1,828 topic views. 1 members, 6 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Everything Mushrooms
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.054 seconds spending 0.004 seconds on 16 queries.