veda_sticks said:

you can make thosuands doing grain to grain transfers

1 jar transfered to 10
10 jars transfered to 100
100 jars transfered t0 a 1000 jars

:-)

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radiantthought said:

veda_sticks said:

you can make thosuands doing grain to grain transfers

1 jar transfered to 10
10 jars transfered to 100
100 jars transfered t0 a 1000 jars

:-)

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I still don't see how that's better than

1 to 160+1
161 to 25921+161

I mean, if we're gonna talk theoretical yields here myc water outpaces g2g at an astronomical rate, even if you can get in 3 g2g cycles per each myc water cycle. Wouldn't senescence set in sooner in g2g as well?

I'm not trying to criticise here, I"m just trying to understand. If you're arguing from pure speed, g2g seems to win hands down, but for bulk myc water seems to be the clear winner to me.

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You can do three G2G transfers no problem,  that is a 1000 jars from one.  It is faster and less chance of contams.  I don't know anyone who needs more than 1000 jars of cubes.  In the long run G2G is the wiser choice unless you can afford to lose jars to contams and senescence should not be in this because it is the same myc if any thing the myc water would break the myc down faster than g2g.

Quote:

So i think 1000 jars would be enough for safety reasons...

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CH HELL said:

You can do three G2G transfers no problem,  that is a 1000 jars from one.  It is faster and less chance of contams.  I don't know anyone who needs more than 1000 jars of cubes.  In the long run G2G is the wiser choice unless you can afford to lose jars to contams and senescence should not be in this because it is the same myc if any thing the myc water would break the myc down faster than g2g.

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CH HELL said:

Even just using a simple GB I have never had 1 jar lost to contam when doing g2g it is just to fast for contams to set in.
While using myc water I have lost several,  the extra moister and slow recovery time just doesn't work for me.  I am not hooked on the GLC,  yes it's easy for a noob but not worth the effort.IME
So I am done with this thread if you like GLC use it but if you have a glove box try g2g.
CH

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No worries mate, I'm just trying to educate myself as well as I can about these things and the voice of experience is always appreciated. As has been proven many times, what seems to make sense in this hobby is not always what is right, and the more info a person can get the better. Thanks.

CH HELL said:

Even just using a simple GB I have never had 1 jar lost to contam when doing g2g it is just to fast for contams to set in.
While using myc water I have lost several,  the extra moister and slow recovery time just doesn't work for me.  I am not hooked on the GLC,  yes it's easy for a noob but not worth the effort.IME
So I am done with this thread if you like GLC use it but if you have a glove box try g2g.
CH

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radiantthought said:

NineInchNails said:

I've done both and I would prefer to do a G2G over the grain LC.

I use regular LCs more often than both because it's simple, effective, and I don't need a GB at all. Just open air work. Ya gotta be clean with the syringe and all that though.

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Why would you need a glovebox for myc water (grain lc) and not normal lc?

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talkingwalnut said:

CH HELL said:

Even just using a simple GB I have never had 1 jar lost to contam when doing g2g it is just to fast for contams to set in.
While using myc water I have lost several,  the extra moister and slow recovery time just doesn't work for me.  I am not hooked on the GLC,  yes it's easy for a noob but not worth the effort.IME
So I am done with this thread if you like GLC use it but if you have a glove box try g2g.
CH

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I've tried 2 LC's and I've never had a successful grow from one.

I've wasted a ton of resources from a LC of PE I was trying to grow out.

I sectored out my cultures drop the agar piece in the grains and g2g that. By doing this I am 100% confident my culture is clean and I've never had a contaminant in my g2g's from doing it that way.

That's getting it done with confidence

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Wow ... I've never tried agar, but have done real well with LCs. Maybe it was just the LC recipe you used. I like dry malt extract with karo, it's the best recipe I've ever used. It's like liquid gold to me now I recently cloned a fruit (using a GB) and dropped 4 pieces of tissue into 4 pints of malt/karo LC and 100% success ... no contam at all.

I've inoculated LCs using PE spores and that shit sprouted overnight literally!

Here's a pic just after inoculation with PE spores. The flecks are just malt sediment:


This is a pic taken just 2 days later:


5 days later:


Here's the recipe if you're interested. If you can do agar then I know this will work for ya.
500 ml pure water (I used RO/DI water)
10 ml Light Karo
1 g Extra Light Malt Extract

Here’s a breakdown of the same recipe for different volumes

600 ml pure water
12 ml of Light Karo
1.2 g Extra Light Dry Malt Extract

250 ml pure water
5 ml of Light Karo
0.5 g Extra Light Dry Malt Extract

I pressure cooked the jars for 30 min @ 15psi

I purchased 1 pound of the extra light dry malt extract on eBay for about $12.00 including shipping. I paid too much, but it was well worth it because 1 pound will likely last a lifetime.

NineInchNails said:

That is an unbelievable amount of growth for just 4 days ... WOW! It usually take me a day or two to see growth on my grain from the LC inoculation.

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