veda_sticks said:

For the thousands time you do not wipe glovebox's down with alcohol. You dont even need to use bleach or lysol etc. Just wipe it down with soapy water.

Alcohol is a big no no, ive seen too many very bad glovebox explosions to know its a bad idea. And i have experienced alcohol vapours ignite even in open air just because a little bit was spilled on the table. the table didnt light, but a huge fireball in the air.

Even flaming outside of a glovebox which you should be doing anyway, its pretty close to your glovebox and theres still a chance alcohol vapours could ignite quite eaisly through the arm holes.

I have a cardboard glovebox, so far i havnt cleaned it, but i will give it a wipe down soon with alcohol when im not using it just because the alcohol will evaporate. At any point if im going to be using it i would never use alcohol.

Its worked perfectly for me since building which is 7 times. which isnt really much of a benchmark. its better than nothing.

If you have varnish, varnish the glovebox then you can wipe it down.



i swear by this, its so easy to work in. The cling film lid makes it so easy to see inside

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Brennus said:

As veda_sticks mentioned, sterility is not required when using a glove box. Glove boxes work on the principal of still air - not sterility.

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mushpunx said:

This is a 7 year old thread dude.


I do see a couple big time errors right off the bat though.

1. You should be using a better filter than micro pore tape. Synthetic filter discs (obviously the best choice) , poly fill, tyvek...  micro pore works fine on Pasty Plates but I would never use it on my grain jars.

2. Yea, youre probably right. If you are putting your unninoculated jars into cardboard flats and then moving them in front of the flow hood without wiping them down that is a huge vector for contamination.

Cardboard tray or not ANYTHING and everything moved in front of a flow hood (or in a still air box) needs to be wiped down absolutely thoroughly with ISO... otherwise you are bringing contams right up in your work space to get blown around.


  When using a flow hood you really need to wipe the table surface down (I use a big glass top)  with ISO. You should run the FH an hour before working. You should shower , put on clean clothes fresh from the dryer, gloves, surgical mask etc. Gloves should be frequently washed with ISO.

Everything, especially jars need to get washed with ISO. I sugguest you download the Letsgrowmushrooms videos and watch RR's sterile tek.

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JohnnyWonder said:

Thank you.  I appreciate your reply.  Yes, this is a 7 year old thread.  And?  Looks like it is still going as I don't think I'm talking to myself here.

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