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Leaf cutting with hormone vs tissue culture?
    #10263043 -

Lets say we have a plant which will possibly root a leaf cutting, but is said not be able to produce shoots thereafter. Now, if I understand properly, tissue culture is able to stimulate shoot growth (from leaf parts etc) by use of certain hormones like cytokinins. But what I don't understand is why a leaf cutting could not be stimulated via this hormone application to also encourage shoot growth.

Therfore, is it probable to use shoot stimulating hormones (just like root growth are used) and get a leaf cutting to produce shoots (in those plants which don't normally do so)? I am trying to avoid the tissue culture approach if possible. It seems like this has been touched on elsewhere, but I never saw a conclusion.


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Re: Leaf cutting with hormone vs tissue culture? [Re: shroomaker]
    #10264244 -

i don't see any reason why it wouldn't work.
BAP can be used to stimulate pupping in cacti and that is not a sterile environment.

try it and see if it works :yesnod:


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Re: Leaf cutting with hormone vs tissue culture? [Re: Dr. uarewotueat]
    #10269649 -

Okay, I think what I am going to do is use rooting hormone to try and get the leaf rooting, and then if that succeeds, apply BAP to try and develop shoots. I don't want to try BAP 'along with' the rooting hormone since it can inhibit adventitious roots.


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