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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Cactus tissue culture. * 1
    #1932583 - 09/19/03 03:32 PM (13 years, 6 months ago)

Here's my first success.

San pedro developing new buds in vitro :laugh:



 


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Offlineneuro
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Re: Cactus tissue culture. [Re: Una]
    #1932590 - 09/19/03 03:33 PM (13 years, 6 months ago)

what's your medium and hormone content and ratio?


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: neuro]
    #1932650 - 09/19/03 03:44 PM (13 years, 6 months ago)

I can not reveal the recipe but if you are interested in cactus tissue culture this document will give some very usefull tips.

http://plant-tc.coafes.umn.edu/listserv/2000/log0010/msg00077.html





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Offlineneuro
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Re: Cactus tissue culture. [Re: Una]
    #1932680 - 09/19/03 03:50 PM (13 years, 6 months ago)

heh i already know how to do cultures and such... back in school i had this shitty lab every tuesday afternoon and all we did was make cultures.. i was just curious as to your recipe...but if mum's the word, then mum's the word  :lipsrsealed:


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InvisibleDazedSol
old hand

Registered: 08/01/01
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Re: Cactus tissue culture. [Re: Una]
    #1933189 - 09/19/03 06:47 PM (13 years, 6 months ago)

Very fucken cool Una!!! :laugh: 

Please keep us updated on your progress.......

This is on my (really long)list of things to do(different, harder to acquire cacti though)......

anywho.
Hehe, I was gonna ask the same thing as neuro but figured the request would be shot down :smile: With good reason though,..there is a lot of money to made on successful culture formula's ....plus all the time done experimenting and all.........thats alot to just give away 


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Invisibletheshiftingwalls
Divine state

Registered: 06/18/03
Posts: 4,128
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Re: Cactus tissue culture. [Re: DazedSol]
    #1933403 - 09/19/03 08:12 PM (13 years, 6 months ago)

Give us the secrect... Or thats just fucked...


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Invisibletheshiftingwalls
Divine state

Registered: 06/18/03
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Re: Cactus tissue culture. [Re: theshiftingwalls]
    #1933408 - 09/19/03 08:14 PM (13 years, 6 months ago)

Malt extract or agar?


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Offlineneuro
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Re: Cactus tissue culture. [Re: theshiftingwalls]
    #1933482 - 09/19/03 08:54 PM (13 years, 6 months ago)

LOL! Not quite!


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Offlinefelixhigh
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Re: Cactus tissue culture. [Re: neuro]
    #1933797 - 09/19/03 11:41 PM (13 years, 6 months ago)

hehehehe
yeah, MEA, then you insert the Cube spores into the mix and there you have a shroomocactus psychodelicus. =)

very very cool UNA!
could you guys please give us more good links on cactus tissue culture? should i treat them like any other plant then (regarding substrate)?


Edited by felixhigh (09/20/03 03:28 AM)


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: felixhigh]
    #1935653 - 09/20/03 04:21 PM (13 years, 6 months ago)

Of course the medium is not MEA.... That's for fungal growth not for plant growth (although malt extraxt is sometimes added to tissue culture media as an 'undefined'  growth factor).

The cactus medium is based on Murashige and Skoog (MS) salts, sugar (sucrose), agar and growth regulators.

I've searched for many hours on the internet for info on TC of cactus but there's not much info out there. The link i posted earlier sums up (practically) all the info that is out there.

Whenever i have the time i will go visit the library of the Agricultural University in Wageningen because i'm sure that many scientific articles have been published about the subject that i can get my hands on there.

As for me not wanting to share the exaxt recipe; This is something we might want to take to a commerical scale (sometimes). In fact i studied horticulture for 4 years and i did my final thesis on the development of plant tissue culture protocols :laugh: 


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Offlineneuro
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Re: Cactus tissue culture. [Re: Una]
    #1935877 - 09/20/03 06:03 PM (13 years, 6 months ago)

>>As for me not wanting to share the exaxt recipe; This is something we might want to take to a commerical scale (sometimes). In fact i studied horticulture for 4 years and i did my final thesis on the development of plant tissue culture protocols

Ahh so this is a carefully investigated recipe.. i understand now..


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Invisibletheshiftingwalls
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Re: Cactus tissue culture. [Re: neuro]
    #1936033 - 09/20/03 07:04 PM (13 years, 6 months ago)

Told you he used agar and malt extract.


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: theshiftingwalls]
    #1937196 - 09/21/03 05:22 AM (13 years, 6 months ago)

Quote:

Told you he used agar and malt extract.
Quote:



I use malt extract for the growth of fungi, haven't used it for TC plants yet. Malt extract is regarded as complex organic supplement of which the effects are not completely understood.



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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: Una]
    #1938225 - 09/21/03 05:29 PM (13 years, 6 months ago)

Here's some more pics.....

more pachanoi



Lophophora developing a new pup



Pereskiopsis rooting stage



oh here's some Salvia divinorum (not a cactus i know)




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Offlineneuro
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Re: Cactus tissue culture. [Re: Una]
    #1938533 - 09/21/03 08:17 PM (13 years, 6 months ago)

looks good una..

have you tried producing a callus then growing shoots or roots off the callus in a separate culture?


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OfflineISH
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Re: Cactus tissue culture. [Re: neuro]
    #1938571 - 09/21/03 08:33 PM (13 years, 6 months ago)

Bad Ass, I'm very interested in seeing your progress.

ISH


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I think of going to the grave without having a psychedelic experience, like going to the grave without having sex. It means that you never figured out what it was all about. The mystery is in the body, and the way the body works itself into nature.
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OnlineAgarico
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Re: Cactus tissue culture. [Re: Una]
    #1938577 - 09/21/03 08:36 PM (13 years, 6 months ago)

Is it possible to do this without  aureoles? Just tissue?  :cool:

Cloning Salvia that way looks like everything else than a productive method  would :lol:     


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InvisibleEffedS
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Registered: 05/15/02
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Re: Cactus tissue culture. [Re: Una]
    #1939019 - 09/21/03 11:34 PM (13 years, 6 months ago)

Una, you are a true innovator.  :thumbup:


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Invisiblepsilocybe cubensis
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Registered: 08/10/00
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Re: Cactus tissue culture. *DELETED* [Re: Effed]
    #1939068 - 09/21/03 11:58 PM (13 years, 6 months ago)

Post deleted by psilocybe cubensis


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OfflineOpenminded
Dicotyledon

Registered: 08/28/03
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Re: Cactus tissue culture. [Re: psilocybe cubensis]
    #1940698 - 09/22/03 02:51 PM (13 years, 6 months ago)

Very, very nice. I've always been fascinated by this sort of tissue culture. I love the way you can simply slice off a bit of something and it will grow into a whole new plant. Well, not quite that simple in practice...
I love growing things, but feel more at home in the lab than in the garden, so this would be a perfect hobby for me!

Please, do keep us informed about your progress. How long did it take for the new shoots to form?


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