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Invisiblespudamore
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Plant tissue culture
    #3410940 - 11/26/04 03:37 AM (12 years, 10 days ago)

want another use for your pressure cooker? or interested in make tissue cultures go here:

http://www.une.edu.au/~agronomy/AgSSrHortTCinfo.html


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Invisibledr_gonz
Registered: 08/18/03
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Re: Plant tissue culture [Re: spudamore]
    #3411055 - 11/26/04 04:20 AM (12 years, 10 days ago)

:thumbup:


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Invisiblespudamore
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Re: Plant tissue culture [Re: dr_gonz]
    #3411388 - 11/26/04 08:49 AM (12 years, 10 days ago)

http://www.jmu.edu/biology/biofac/facfro/cloning/cloning.htm

http://www.palouse.net/kitchenculture/tcinfo.htm#Basics

half way down on this page is more medium recipes and another how to

http://www.aquabotanic.com/stiffaquatic.htm

please post more medium recipes if anybody finds them.


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Edited by spudamore (11/26/04 09:03 AM)


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InvisibleStonehenge
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Re: Plant tissue culture [Re: spudamore]
    #3411640 - 11/26/04 11:34 AM (12 years, 10 days ago)

I've tried it and while that certainly doesn't make me an expert on the subject, I will say that it's harder than it looks. The trouble is contamination. It's hard to get your plant cuttings sterile without killing them. Then, the other hard parts are the fact you have to open the culture jar to add nutes or to transfer to other jars which gives more chances for contamination. I had constant contams which doomed my effort. I had done shrooms and thought my sterile technique was reasonably good. I found out later talking to someone else who did it that it was probably dust mites that made most of the contams. They just walk right in and you have to leave a crack for air so they walk in and contaminate your culture. Next time I'll use some sticky stuff to keep them out. I had a home made glovebox which you do need. You can make one out of a cardboard box and plastic sheet. Use lysol spray for disinfecting.


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Invisiblespudamore
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Re: Plant tissue culture [Re: Stonehenge]
    #3412932 - 11/26/04 06:07 PM (12 years, 10 days ago)

sweet as, not going to try till after a few weeks anyways. where abouts do you store the jars? also in your jars do you have to allow for gas exchange?


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InvisibleStonehenge
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Re: Plant tissue culture [Re: spudamore]
    #3413374 - 11/26/04 08:15 PM (12 years, 10 days ago)

The jars I used were baby food jars with special neoprene lids. The lids are designed so that they leave a tiny gap, less than a millimeter but it's enough for some gas exchange. The gap points downward so dust particles do not tend to go inside the jar. It's really very well designed. I have some extra jars and neoprene lids but I'd have to get something for them.

You can put the jars pretty much anywhere but in a glovebox is one good place. You could put them on a shelf in a cabinet. Wipe it out with antiseptic before you store any jars there. You do have to watch the temps. The nice thing about the neoprene lids is that they let light in. This is important when you are culturing plants. At times you don't want them to have any light so you just keep them in the dark. When they do need light for photosynthesis then you just put them under one and it gets right to them. You can even stack the jars. I plan to get back into it but I have too many projects going on right now already so it'll be later on for me.


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“A democracy cannot exist as a permanent form of government. It can only exist until the voters discover that they can vote themselves largesse from the public treasury. From that moment on, the majority always votes for the candidates promising the most benefits from the public treasury with the result that a democracy always collapses over loose fiscal policy, always followed by a dictatorship.” (attributed to Alexis de Tocqueville political philosopher Circa 1835)

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Invisiblespudamore
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Re: Plant tissue culture [Re: Stonehenge]
    #3413412 - 11/26/04 08:23 PM (12 years, 10 days ago)

one last question for ya.

does the plant tissue have to be fresh? how old before you cannot use plant tissue?


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InvisibleStonehenge
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Re: Plant tissue culture [Re: spudamore]
    #3415437 - 11/27/04 12:09 PM (12 years, 9 days ago)

Always use fresh plant material. It really depends, even if a plant can live a long time after cutting, like pedro for example, you still want to cut it fresh because the cut end will have started to callous over if it's been sitting a while and you don't want that. If it's seriously wilted that's not good either. I always used freshly cut plant material and that seemed best. Another thing you have to watch for is your culture medium can skin over. I used a little bit of agar in it and if you just put the plant cutting on the surface it may not get the moisture it needs. I found it worked better to press the cutting into the medium so that it made good contact or was slightly under the surface at some point. There are lots of other details, you can get the formulas and procedures from one of those links.


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“A democracy cannot exist as a permanent form of government. It can only exist until the voters discover that they can vote themselves largesse from the public treasury. From that moment on, the majority always votes for the candidates promising the most benefits from the public treasury with the result that a democracy always collapses over loose fiscal policy, always followed by a dictatorship.” (attributed to Alexis de Tocqueville political philosopher Circa 1835)

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InvisibleJohn
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Re: Plant tissue culture [Re: Stonehenge]
    #3415807 - 11/27/04 02:32 PM (12 years, 9 days ago)

i use a homemade glove box with no problems, you need hormones though, recipes that use coconut milk ect are crappy and hardly work well. another thing is a lot of plants require diffrent mediums and some plants won't respond to tc well at all, i suggest researching the specfic plant you want to culture and the media needs for it. i'm in a microprop class and we have about 100 diffrent medias for various plants :stoned:



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Invisiblespudamore
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Re: Plant tissue culture [Re: John]
    #3417283 - 11/27/04 09:42 PM (12 years, 8 days ago)

a long list of links on this page

http://www.cannabinoid.com/boards/msg9x12789.shtml


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OfflineGr0wer
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Re: Plant tissue culture [Re: spudamore]
    #3417682 - 11/27/04 11:54 PM (12 years, 8 days ago)

sticky?


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Invisiblespudamore
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Re: Plant tissue culture [Re: Gr0wer]
    #3418030 - 11/28/04 01:59 AM (12 years, 8 days ago)



?????


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Mushrooms, Mycology and Psychedelics >> The Ethnobotanical Garden

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