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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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need a good peroxide agar recipe
    #9865789 -

Hi
Im a noob and Im really trying to cut my contam risks down to a minimum with peroxide.

so heres what Ive got:
nutritional yeast
health food store agar agar
honey
karo
box o potato flakes...
so can I add h2o2 to that for to start isolating a rhizo?

..and if so, can I have a good recipe with measurements to fill about 20-30 petri dishes?

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Re: need a good peroxide agar recipe [Re: thinkn]
    #9865871 -

You shouldnt need it unless your working with a really badly contaminated culture.

If your doing simple agar work from indoor grown fruits, or syringes that are trusted. Regular agar will work fine, and you can always transfer away from contamants.

Sterile procedures are the key.


--------------------

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Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Re: need a good peroxide agar recipe [Re: veda_sticks]
    #9865913 -

http://www.shroomery.org/forums/showflat.php/Number/466150#466150

Peroxide Agar Tek

basically wait for agar to cool below the temp where peroxide breaks down and then add it to the agar, stir/swirl and pour the plates.


--------------------
Hot Water Pasteuriztion - A How To Pictorial
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4527808/an/0/page/0

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http://www.shroomery.org/forums/showflat.php/Cat/0/Number/4500682/an/0/page/5

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Make Your Own Magnetic Stirrer! How-To Tek w/ pix
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5865949/an/0/page/3

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Re: need a good peroxide agar recipe [Re: No_Life_G33k]
    #9866011 -

thanks but I've already read that posting, that's what kinda got me interested.
Thing is I live in the inner city or rather "da hood"
unfortunately there are no home brew shops anywhere so malt extract and dextrose are not an option. The list of ingredients that I gave above is all that's available to me.
So I just wanted to know if I can add h2o2 and if so what amounts?

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Re: need a good peroxide agar recipe [Re: thinkn]
    #9866091 -

:thumbup:


--------------------
"So uh, Randy? You wanna go in the men's room and I'll uh, ...:)give you a hand-job?":stonedjerk:
-South Park

A guy goes to the doctor, and asks "what's wrong with me; I'm sick and have trouble remembering stuff." Doctor does a few tests, goes to the man a few hours later and says, "I've got some bad news; you've got cancer, and you've got alzheimers." The man looks at the doctor and says, "Phew! At least I don't have cancer!":)

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Re: need a good peroxide agar recipe [Re: abowers1988]
    #9866222 -

lol thumbs up? back at ya,lol

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Re: need a good peroxide agar recipe [Re: thinkn]
    #9867561 -

just use potato and dextrose.

or use dog food agar if you can't manage to locate some dextrose.

H2O2 dosage is in the tek.

eta
http://www.bodybuilding.com/store/now/dex.html

dextrose is $4ish for 2 pounds

should also be able to find it at an health food /organic grocer type store or a supplement store.

Edited by No_Life_G33k (02/26/09 02:00 AM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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