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InvisibleFractalXplora
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-=Hydrogen Peroxide Agar tek =- advice needed_-=<3
    #5511114 - 04/13/06 04:43 AM (17 years, 11 months ago)

Hello all,I want to try cloning using peroxide agar tek, got some PDA and dishes and am ready to go. In the tek i read  it says 1ml of 3% peroxide to 150ml of water, the stuff i got is 6% solution so i imagine i should just whack 0.5ml in? Sorry if tis is  a daft question, just dont wanna screw it up over something simple, thanks<> :royalrainbow:


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InvisibleFractalXplora
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Re: -=Hydrogen Peroxide Agar tek =- advice needed_-=<3 [Re: FractalXplora]
    #5511269 - 04/13/06 06:44 AM (17 years, 11 months ago)

also is it safe to add the peroxide without a glovebox, just in a small well bleached room? cheers..


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OfflineRogerRabbitM
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Re: -=Hydrogen Peroxide Agar tek =- advice needed_-=<3 [Re: FractalXplora]
    #5511443 - 04/13/06 08:30 AM (17 years, 11 months ago)

No. Agar work requires a glovebox or flowhood. You'll also need the glovebox to pour petri dishes. Be sure to let the agar cool before adding the peroxide. You can also dip the piece of mycelium you cut into peroxide, but if you use a flame sterilized scalpel and only transfer material from the center of the stipe or cap that hasn't been exposed to air, the peroxide isn't needed.
RR


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InvisibleFractalXplora
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Re: -=Hydrogen Peroxide Agar tek =- advice needed_-=<3 [Re: RogerRabbit]
    #5511665 - 04/13/06 09:45 AM (17 years, 11 months ago)

ok cheers RR but wat about using 6% peroxide do i only use 0.5cc?

cant find 3% peroxide over here.


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OfflineRogerRabbitM
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Re: -=Hydrogen Peroxide Agar tek =- advice needed_-=<3 [Re: FractalXplora]
    #5511791 - 04/13/06 10:27 AM (17 years, 11 months ago)

Yea, cut it in half with sterilized distilled water, or use half the amount.
RR


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