Home | Community | Message Board


Kratom Eye
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy CBD, Cannabis Seeds   Amazon ½ Pint Jars, HEPA Filter, Pressure Cooker

Jump to first unread post. Pages: 1
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
how to make a sporesyringe (pictorial)
    #879865 - 09/12/02 06:59 AM (14 years, 9 months ago)

This document can be found on our new website, but i hadn?t posted it here yet.

Making a sporesyringe


although the photographs show the procedure in the open air we make all sporesyringes in a flow cabinet!

To make a sporesryinge you will need the following items:

*Inoculation loop/scalpel (something to pick up the spores)
*Sporeprint
*Syringe and needle (sterile)
*Small amount of sterilised water in a bottle (capped with tinfoil)
*Clean flame source (gas- or alcohol burner)
*Latex gloves (optional)




The most important thing in the whole process is to work clean. If possible perform the following procedure in a glovebox or HEPA filtered environment. If you do not have access to one of these you can perform it in the open air, but chances of succes or smaller. If available wear gloves that have been desinfected with alcohol 70% or lysol.
CAUTION: Most desinfectants are flammable, use extreme caution when using these in combination with an open flame!

Before you start clean the working surface with alcohol or lysol. Allow it to dry before proceding!




The inoculation loop or scalpel is flamed until red hot, and left to cool. Alternatively you can use a presterilised inoculation loop.



Peal back the tinfoil of the flask so that it can be removed easily but leave it covering the flask. Open the baggie with the sporeprint and scrape some of the spores of the paper. If the loop is too hot the spores will be killed or the plastic will melt to it.:



Deposit the spores into the water and quickly replace the tinfoil. Repeat if not enough spores have been transferred. If the spores are visible in the water there should be enough.




The syringe is assembled. Most syringes can be reused by sterilising them in a pressure cooker. Just wrap them in tinfoil and sterilise for 25-30 minutes.



Some spore suspension is sucked up in the syringe. Pull the plunger back and forth a few times to disperse the spores in the water. Syringes can then be filled with sporesuspension.



The needle cap is replaced and the syringes are put in the fridge until ready to use. Don?t forget to lable them with strain and date. Syringes can be kept like this for several months after which they become noticably less viable.




--------------------
www.911blogger.com


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: how to make a sporesyringe (pictorial) [Re: Una]
    #879868 - 09/12/02 06:59 AM (14 years, 9 months ago)

Very nice Una. Thank you for sharing that document with the forum!


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemile69
wonderer/wanderer ૐ
 User Gallery

Registered: 07/06/02
Posts: 967
Loc: other side of the world
Last seen: 1 year, 11 days
Re: how to make a sporesyringe (pictorial) [Re: Una]
    #879921 - 09/12/02 07:23 AM (14 years, 9 months ago)

yeah una, this was really informative.  you should make this either sticky for a while, or just bump this periodically.  its way easier to follow directions if you have the whole step by step process with pictures:) thanks alot :grin:


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: how to make a sporesyringe (pictorial) [Re: mile69]
    #879927 - 09/12/02 07:26 AM (14 years, 9 months ago)

I made it sticky right after I replied.. :wink: 


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRipple
Ripple
Male User Gallery

Folding@home Statistics
Registered: 05/16/02
Posts: 21,014
Loc: the timbers of Fennario
Trusted Cultivator
Re: how to make a sporesyringe (pictorial) [Re: Una]
    #880018 - 09/12/02 08:28 AM (14 years, 9 months ago)

Very nice and thanks, Just a question about the length of time the syringe stays healthy. I had read that if kept in a cool dark the syringe can remain viable for much longer. And is the fridge really necessary?


--------------------
The bus came by and I got on that's when it all began!



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemile69
wonderer/wanderer ૐ
 User Gallery

Registered: 07/06/02
Posts: 967
Loc: other side of the world
Last seen: 1 year, 11 days
Re: how to make a sporesyringe (pictorial) [Re: mickey_rourke]
    #880197 - 09/12/02 10:41 AM (14 years, 9 months ago)

ahhh...sorry :smirk:, i must have missed it:)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDuzted
the next misscleo

Registered: 08/09/02
Posts: 30
Last seen: 14 years, 7 months
Re: how to make a sporesyringe (pictorial) [Re: mile69]
    #880223 - 09/12/02 11:05 AM (14 years, 9 months ago)

Well that sounds alittle too much work to me. Recently I started 4 jars from a spore syringe I made and this is all I did

got a cup and filled it 1\8 to the top with bottled water.
scraped spores off foil with a knife.
shook the scraped pours into the cup.
put some plastic wrap over the cup and shook it up real well.
got my syringe(which I NEVER flamed sterlized) and sucked up the spore water and inoculated all my jars.
Never had a problem with contams.

Guess I'm just lucky  :grin:

More like stupid I should be more careful but oh well


--------------------
________________________________
Be a shroom-head


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: how to make a sporesyringe (pictorial) [Re: Una]
    #880658 - 09/12/02 03:23 PM (14 years, 9 months ago)

Una,

Great, as always............... :wink:

Smile, life is more than this screen.



6T  :tongue:

   


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinebluesky
mushroom cowboy

Registered: 09/04/02
Posts: 561
Last seen: 14 years, 14 hours
Re: how to make a sporesyringe (pictorial) [Re: SixTango]
    #880668 - 09/12/02 03:30 PM (14 years, 9 months ago)

hey, nice. Ive always wondered how to do that!!!! Thanks a lot!! :grin: 


--------------------
You're my blue sky, you're my sunny day,
Lord you know it makes me high when you turn your love my way. Turn your love my waaaaaay, Yea.
-Richard (Dickey) Betts


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDarkmoon
Thinker

Registered: 11/18/99
Posts: 218
Re: how to make a sporesyringe (pictorial) [Re: Una]
    #880746 - 09/12/02 04:23 PM (14 years, 9 months ago)

Super Post, with great detail, im sure it will help many people!!  Thanks Una! :laugh: 


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMr_Puke
Suicidal Failure

Registered: 06/11/02
Posts: 673
Last seen: 13 years, 8 months
Re: how to make a sporesyringe (pictorial) [Re: Darkmoon]
    #881064 - 09/12/02 10:10 PM (14 years, 9 months ago)

i wish i had all those cool lil instruments things that you usin that there  :confused:


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: how to make a sporesyringe (pictorial) [Re: Una]
    #881680 - 09/13/02 10:39 AM (14 years, 9 months ago)

EXCELLLENNTT!!!!!!!!!!!!!
This should be included in the FAQS or constantly right here on top of the posts list . Many "SHROOMS UP ! " to this fine piece of info . :laugh: :laugh: :cool: 


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 14 hours, 1 minute
Re: how to make a sporesyringe (pictorial) [Re: tripndicular]
    #882347 - 09/13/02 04:20 PM (14 years, 9 months ago)

>This should be included in the FAQS

Will be.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoctorJ
Stranger
 Arcade Champion: Frogger

Registered: 06/30/03
Posts: 8,451
Loc: space
Re: how to make a sporesyringe (pictorial) [Re: Anno]
    #2172910 - 12/12/03 02:05 PM (13 years, 6 months ago)

you can make your own innoculation loop with a paperclip!

and you can use a butane lighter instead of a bunsen burner!

Great FAQ, just wanted to add these low-tech replacements for expensive/hard-to-find materials.


--------------------
peace, pot, and microdot!


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleniteowl
GrandPaw
Male User Gallery

Registered: 07/01/03
Posts: 16,291
Loc: Flag
Re: how to make a sporesyringe (pictorial) [Re: DoctorJ]
    #2175372 - 12/13/03 09:03 PM (13 years, 6 months ago)

You can flame the needle and use it to scrape the spores off with.


--------------------
Live for the moment you are in now
Don't be bogged down by your past
Don't be afraid of what lies in your future


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePsilocybeingzz
Male User Gallery

Registered: 12/15/02
Posts: 14,463
Loc: International waters
Last seen: 4 years, 7 months
Re: how to make a sporesyringe (pictorial) [Re: niteowl]
    #2175393 - 12/13/03 09:26 PM (13 years, 6 months ago)

From now on I am only buying strains of spores I DONT HAVE!!!!!! HA HA HA :smile:

I to was waiting to learn how to do this, I was just never looked it up, I knew it would be pretty easy!!!
"Well that sounds alittle too much work to me. Recently I started 4 jars from a spore syringe I made and this is all I did

got a cup and filled it 1\8 to the top with bottled water.
scraped spores off foil with a knife.
shook the scraped pours into the cup.
put some plastic wrap over the cup and shook it up real well.
got my syringe(which I NEVER flamed sterlized) and sucked up the spore water and inoculated all my jars.
Never had a problem with contams.

Guess I'm just lucky "



Yes maybe but does it really hurt to flame sterilize???, like does it really take that much money or effort????

Yo gave me an idea to!!, rub the needle with alcohol, flame it off cool the needle and stick the needle THROUGH the plastic wrap(or tinfoil) and suck up the water , thats even cleaner


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy CBD, Cannabis Seeds   Amazon ½ Pint Jars, HEPA Filter, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* spore printing: Index Cards, or Tinfoil?
( 1 2 all )
TripleB 1,898 22 05/26/04 12:35 AM
by TripleB
* sterilizing vakuum sealed scalpell/inoculation loop ... ohmatic 763 2 12/12/04 12:34 PM
by ohmatic
* need good LC tek pictorial cpw1971 2,678 10 12/11/06 09:57 PM
by odium33
* LC tek pictorial Kukukatcho 1,616 8 08/04/08 06:56 AM
by risndeath
* Alcohol-sprayed glovebox: how to sterilize scalpel? vzalkaloid 2,007 3 07/14/03 03:14 PM
by vzalkaloid
* flaming scalpels and tweezers
( 1 2 all )
chobumms 2,201 21 07/08/08 11:36 PM
by BrandNewbie
* My Favorite Scalpel...is not a Scalpel Peterthinks 1,545 19 03/27/08 11:07 AM
by tahoe
* Deleted
( 1 2 3 all )
Snaggletooth 5,497 52 06/08/06 11:14 PM
by rxKaffee

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
8,107 topic views. 19 members, 62 guests and 33 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Shroom Supply
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.041 seconds spending 0.004 seconds on 19 queries.