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Nuggetpouch


Registered: 08/07/04
Posts: 2,035
Last seen: 10 years, 9 months
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Sterlizing Jars Twice
#2992122 - 08/11/04 04:59 PM (19 years, 5 months ago) |
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Unfortunately I have no access to the luxuries of a pressure cooker so I must steam sterlize, however I have read that steam sterlization is often unsuccesful, I would like to know if stean sterlizing my jars twice is safe or maybe if it will help decrease chances of contamination?
-------------------- My statistical assumption is that chemical tapering should begin when the rate of visiting ODD exceeds the rate of visiting Pub, or any other Shroomery forum.
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fugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 13 years, 6 months
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Re: Sterlizing Jars Twice [Re: Nuggetpouch]
#2992158 - 08/11/04 05:09 PM (19 years, 5 months ago) |
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what do you wan t to sterialize
-------------------- mushroom culture history making ...Mr. Allan is the best .....
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Nuggetpouch


Registered: 08/07/04
Posts: 2,035
Last seen: 10 years, 9 months
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Re: Sterlizing Jars Twice [Re: Nuggetpouch]
#2992166 - 08/11/04 05:12 PM (19 years, 5 months ago) |
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about 4-5 jars of pf style Vermiculite/BFL filled up in glass cups with a tin foil lid
-------------------- My statistical assumption is that chemical tapering should begin when the rate of visiting ODD exceeds the rate of visiting Pub, or any other Shroomery forum.
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mycopsycho
Tit Inspector.


Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
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Re: Sterlizing Jars Twice [Re: Nuggetpouch]
#2992245 - 08/11/04 05:29 PM (19 years, 5 months ago) |
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boil them three times, with about 8-12 hours in between. this is called fractional sterilization.
-------------------- I Am The Sickness. Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.
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Philanthropist
Savior ofMankind

Registered: 06/01/04
Posts: 204
Loc: Amsterdam, Holland
Last seen: 14 years, 1 month
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Re: Sterlizing Jars Twice [Re: mycopsycho]
#2992716 - 08/11/04 06:55 PM (19 years, 5 months ago) |
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I love you guys, i only do mine once and i always get a few contams!
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Spudz76
Got mycelium on the brain...


Registered: 02/08/04
Posts: 391
Last seen: 13 years, 10 months
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Yeah the second and third with hours in between allow for undead spores/bacteria left over from previous steps to germinate, and then you heat them again and it kills the newly born contams (they die easier once they 'hatch', but before they latch on - think "contam fetuses"). Doing this three times generally whacks em all. Just for the science behind it.
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Hangnail
Teo Torriatte

Registered: 03/03/04
Posts: 129
Loc: Over at the Frankenstein ...
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Re: Sterlizing Jars Twice [Re: Nuggetpouch]
#2993879 - 08/11/04 11:24 PM (19 years, 5 months ago) |
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If they're whole grains yes. My crew did hundreds of pf jars with steam only back in the day. Never lost one to poor sterilization.
-------------------- tonight you'll fall asleep in clothes-so late like a candy bar wrapped up for lunch that's all you get to taste poverty and spit poverty and spit
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KonkeyDong
newbie
Registered: 07/07/04
Posts: 45
Last seen: 19 years, 5 months
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Re: Sterlizing Jars Twice [Re: Nuggetpouch]
#2994106 - 08/12/04 12:16 AM (19 years, 5 months ago) |
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Make sure you presoak the grain for a while (I like 12 hours). This will get most of the bacteria to germinate so you can burn the shit out of it with your steam bath.
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Legoulash
Stranger

Registered: 09/07/02
Posts: 4,347
Last seen: 12 years, 7 months
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Re: Sterlizing Jars Twice [Re: KonkeyDong]
#2994121 - 08/12/04 12:20 AM (19 years, 5 months ago) |
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Is it me or is Mush cult going down hill?
(That wasnt directed to you nuggetpouch)
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mycopsycho
Tit Inspector.


Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
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Re: Sterlizing Jars Twice [Re: Legoulash]
#2996157 - 08/12/04 03:09 PM (19 years, 5 months ago) |
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what do you mean down hill? this is where the n00bs start so technically this is the bottom of the hill?!?!?!?!
-------------------- I Am The Sickness. Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.
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CactusMonkey
Answers only toCthulu

Registered: 07/01/04
Posts: 45
Last seen: 19 years, 4 months
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Re: Sterlizing Jars Twice [Re: mycopsycho]
#2996184 - 08/12/04 03:15 PM (19 years, 5 months ago) |
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You don't think that you can over cook the substrate thus burning up the nutrients?
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Fucknuckle
Dog Lover

Registered: 04/24/04
Posts: 6,762
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Re: Sterlizing Jars Twice [Re: Hangnail]
#2996201 - 08/12/04 03:19 PM (19 years, 5 months ago) |
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I have a real hard time believing you never had any bad jars with boiling. As a matter of fact your the first person I have ever heard saying that! Goodluck
-------------------- What it is, is what it is my Brother. It is as it is, so suffer thru it.
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