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OfflineNuggetpouch
Male

Registered: 08/08/04
Posts: 2,035
Last seen: 9 years, 7 months
Sterlizing Jars Twice
    #2992122 - 08/12/04 01:59 AM (18 years, 3 months ago)

Unfortunately I have no access to the luxuries of a pressure cooker so I must steam sterlize, however I have read that steam sterlization is often unsuccesful, I would like to know if stean sterlizing my jars twice is safe or maybe if it will help decrease chances of contamination?


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My statistical assumption is that chemical tapering should begin when the rate of visiting ODD exceeds the rate of visiting Pub, or any other Shroomery forum.


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Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 12 years, 4 months
Re: Sterlizing Jars Twice [Re: Nuggetpouch]
    #2992158 - 08/12/04 02:09 AM (18 years, 3 months ago)

what do you wan t to sterialize


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mushroom culture history making ...Mr. Allan is the best .....


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OfflineNuggetpouch
Male

Registered: 08/08/04
Posts: 2,035
Last seen: 9 years, 7 months
Re: Sterlizing Jars Twice [Re: Nuggetpouch]
    #2992166 - 08/12/04 02:12 AM (18 years, 3 months ago)

about 4-5 jars of pf style Vermiculite/BFL filled up in glass cups with a tin foil lid


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My statistical assumption is that chemical tapering should begin when the rate of visiting ODD exceeds the rate of visiting Pub, or any other Shroomery forum.


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Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/18/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: Sterlizing Jars Twice [Re: Nuggetpouch]
    #2992245 - 08/12/04 02:29 AM (18 years, 3 months ago)

boil them three times, with about 8-12 hours in between. this is called fractional sterilization.


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I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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OfflinePhilanthropist
Savior ofMankind

Registered: 06/01/04
Posts: 204
Loc: Amsterdam, Holland
Last seen: 13 years, 6 days
Re: Sterlizing Jars Twice [Re: mycopsycho]
    #2992716 - 08/12/04 03:55 AM (18 years, 3 months ago)

I love you guys, i only do mine once and i always get a few contams!


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OfflineSpudz76
Got mycelium on the brain...
Male

Registered: 02/08/04
Posts: 391
Last seen: 12 years, 8 months
Re: Sterlizing Jars Twice [Re: Philanthropist]
    #2993824 - 08/12/04 08:12 AM (18 years, 3 months ago)

Yeah the second and third with hours in between allow for undead spores/bacteria left over from previous steps to germinate, and then you heat them again and it kills the newly born contams (they die easier once they 'hatch', but before they latch on - think "contam fetuses"). Doing this three times generally whacks em all. Just for the science behind it.


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InvisibleHangnail
Teo Torriatte

Registered: 03/03/04
Posts: 129
Loc: Over at the Frankenstein ...
Re: Sterlizing Jars Twice [Re: Nuggetpouch]
    #2993879 - 08/12/04 08:24 AM (18 years, 3 months ago)

If they're whole grains yes. My crew did hundreds of pf jars with steam only back in the day. Never lost one to poor sterilization.


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tonight you'll fall asleep in clothes-so late like a candy bar wrapped up for lunch that's all you get to taste
poverty and spit
poverty and spit


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OfflineKonkeyDong
newbie
Registered: 07/08/04
Posts: 45
Last seen: 18 years, 3 months
Re: Sterlizing Jars Twice [Re: Nuggetpouch]
    #2994106 - 08/12/04 09:16 AM (18 years, 3 months ago)

Make sure you presoak the grain for a while (I like 12 hours). This will get most of the bacteria to germinate so you can burn the shit out of it with your steam bath.


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OfflineLegoulash
Stranger
 User Gallery
Registered: 09/07/02
Posts: 4,347
Last seen: 11 years, 5 months
Re: Sterlizing Jars Twice [Re: KonkeyDong]
    #2994121 - 08/12/04 09:20 AM (18 years, 3 months ago)

Is it me or is Mush cult going down hill?

(That wasnt directed to you nuggetpouch)


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Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/18/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: Sterlizing Jars Twice [Re: Legoulash]
    #2996157 - 08/13/04 12:09 AM (18 years, 3 months ago)

what do you mean down hill? this is where the n00bs start so technically this is the bottom of the hill?!?!?!?!


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I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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OfflineCactusMonkey
Answers only toCthulu

Registered: 07/02/04
Posts: 45
Last seen: 18 years, 2 months
Re: Sterlizing Jars Twice [Re: mycopsycho]
    #2996184 - 08/13/04 12:15 AM (18 years, 3 months ago)

You don't think that you can over cook the substrate thus burning up the nutrients?


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InvisibleFucknuckle
Dog Lover

Registered: 04/25/04
Posts: 6,762
Re: Sterlizing Jars Twice [Re: Hangnail]
    #2996201 - 08/13/04 12:19 AM (18 years, 3 months ago)

I have a real hard time believing you never had any bad jars with boiling. As a matter of fact your the first person I have ever heard saying that!  Goodluck

  :thumbdown:


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What it is, is what it is my Brother.
It is as it is, so suffer thru it.


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