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i believe that the mycelium will become more rhizomorphic, and grow more rapidly after it becomes dykariotyk. also the qualities of mycelium varies per strain as shown in the TMC. I dont think that the 4 month time would effect the spores negativly in any way.
-------------------- Completely straightup downright digging it. Can you dig it?
Re: rhizomorphic versus cottony growth on agar #86306 - 06/17/00 06:23 AM (18 years, 10 months ago)
This is extremely common when introducing spores onto agar medium. You have tormentose, all you need to do is perform 1-2 rhizomorphic isolations. This seems difficult because all you see is fuzz, and the entire plate could grow out that way, with a few ropes finally appearing towards the end. This generally occurs by adding too many spores; you get a massive lawn of monokaryons, and it takes a while for them to get sorted out. Anyway, take a small piece of fuzz/mycelia, from the outer growing edge and put it in the center of a fresh petri. Depending on the amount of spores you started with, this may take more than 1 plate to isolate only rhizomorphs. The second plate will contain aprox. half fuzz, and half ropes. Transfer a growing edge of ropey mycelia to a fresh petri, and hopefully this plate will be pure ropes. Now for the good news.... Unless you need to sector a pure strain.... IT DON'T MATTER! Your petri is fine for all intents and purposes, whether it be for a transfer, PDY, or sterile water inoculant. If you get tons of aborts, or just a few mushrooms, or no mushrooms at all, well then that is a possible sign that your strain has degenerated. Hope this helps!
What little I know....
Re: rhizomorphic versus cottony growth on agar #86307 - 06/17/00 09:23 AM (18 years, 10 months ago)
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