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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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OfflineDragonAlienFest1
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Registered: 03/31/00
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Last seen: 17 years, 4 months
Re: rhizomorphic versus cottony growth on agar
    #86304 - 06/17/00 12:33 AM (23 years, 5 months ago)

i believe that the mycelium will become more rhizomorphic, and grow more rapidly after it becomes dykariotyk. also the qualities of mycelium varies per strain as shown in the TMC. I dont think that the 4 month time would effect the spores negativly in any way.


--------------------
Completely straightup downright digging it. Can you dig it?


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Anonymous

Re: rhizomorphic versus cottony growth on agar [Re: DragonAlienFest1]
    #86306 - 06/17/00 04:23 AM (23 years, 5 months ago)

This is extremely common when introducing spores onto agar medium. You have tormentose, all you need to do is perform 1-2 rhizomorphic isolations.
This seems difficult because all you see is fuzz, and the entire plate could grow out that way, with a few ropes finally appearing towards the end. This generally occurs by adding too many spores; you get a massive lawn of monokaryons, and it takes a while for them to get sorted out.
Anyway, take a small piece of fuzz/mycelia, from the outer growing edge and put it in the center of a fresh petri.
Depending on the amount of spores you started with, this may take more than 1 plate to isolate only rhizomorphs.
The second plate will contain aprox. half fuzz, and half ropes. Transfer a growing edge of ropey mycelia to a fresh petri, and hopefully this plate will be pure ropes.
Now for the good news....
Unless you need to sector a pure strain....
IT DON'T MATTER!
Your petri is fine for all intents and purposes, whether it be for a transfer, PDY, or sterile water inoculant.
If you get tons of aborts, or just a few mushrooms, or no mushrooms at all, well then that is a possible sign that your strain has degenerated.
Hope this helps!

------------------

What little I know....



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Anonymous

Re: rhizomorphic versus cottony growth on agar [Re: DragonAlienFest1]
    #86307 - 06/17/00 07:23 AM (23 years, 5 months ago)

Thanks for the post. BIG TIME help!

Sterile water was used to innoculate jars of bird seed. Today (this morning in fact), I see some strandy sections.

I guess I was just hoping for fast growth. Impatient person that I am.

I'm thinking it would be wise to isolate some of the strands that I see growing in the jar?

Later, Tor



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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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