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InvisibleOlgualion
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Re: wild P. mexicana - finally some nice growth! :) [Re: zeronio]
    #1386607 - 03/18/03 03:00 AM (21 years, 4 months ago)

Out of curiosity, where did your P. mex come from?


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Offlinezeronio
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Re: wild P. mexicana - finally some nice growth! :) [Re: Olgualion]
    #1386654 - 03/18/03 03:25 AM (21 years, 4 months ago)

Some came from MushMush. The mexicana, tampanensis & atlantis have a beautiful and very similar looking mycelium. They're pictured in "The mushroom cultivator".

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InvisibleOlgualion
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Re: wild P. mexicana - finally some nice growth! :) [Re: zeronio]
    #1386785 - 03/18/03 04:20 AM (21 years, 4 months ago)

My buddy has experience with tampanensis, and I have posted a few pics.  I love the orangish color that it has, and IMO it is a very beautiful mycelium (my favorite). 

My bud has a few bags of r.g.s. colonising with mex A strain, and all I have noticed is white.  Maybe the other colors come more with age?  This is not the case IME with tampanensis, which upon spore germination quickly shows its colors.

I haven't ever seen any P. atlantis mycelium, but would love to.  Do you have a print? :wink:  Could you post a pic of some your mexicana mycelium on agar? 


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OfflineAnnoA
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Re: wild P. mexicana - finally some nice growth! :) [Re: Olgualion]
    #1386829 - 03/18/03 04:37 AM (21 years, 4 months ago)

P. mexicana on PDYA:


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InvisibleJoshua
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Re: wild P. mexicana - finally some nice growth! :) [Re: Anno]
    #1387256 - 03/18/03 07:11 AM (21 years, 4 months ago)

P. mexicana on peroxidated DFA. Note sclerotia formation and lack of multi-colored zonation.



The zonate comment was intended for zeronio

Joshua


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Edited by Joshua (03/18/03 07:16 AM)

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InvisibleOlgualion
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Re: wild P. mexicana - finally some nice growth! :) [Re: Anno]
    #1388880 - 03/18/03 04:09 PM (21 years, 4 months ago)

thanks for the pics guys!

Joshua, your pic makes me think of an idea I had in the past when playing with the tamps. Notice how the sclerotia seem to form more readily when they reach a non nutritious barrier ie. the plastic separations of the dish, or the sides of a glass jar... Well this made me think that maybe the addition of pieces of rock, or marbles, or some other such non nutritious additive would help with formation of stones say in the center of a bag of r.g.s. What do you guys think?

What made me think of this was when dude separated through his old tray of tamp substrate he noticed that almost all of the stones that were inside the mycelial block were formed on and sometimes even in sunflower seed shells (a non-nutritious or at least minimally nutritious "barrier").


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Edited by Olgualion (03/18/03 04:13 PM)

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Offlinezeronio
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Re: wild P. mexicana - finally some nice growth! :) [Re: Joshua]
    #1389526 - 03/18/03 09:07 PM (21 years, 4 months ago)

Yes, the zones are not always very obvious. I think that they occur because of different speed of growth of mycellium. At least my cultures have periods of faster and slower growth.
I'm going to try to make a time lapse video next time.

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OfflineRaadt
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Re: wild P. mexicana - finally some nice growth! :) [Re: Olgualion]
    #1390224 - 03/19/03 04:26 AM (21 years, 4 months ago)

I was actually thinking the exact same thing, seeing as stones are usually found in a casing layer (non-nutritious).. it leads me to believe if you added a large ammount of non-nutritious substrate you might have higher stone formation levels. Marbles would probably work well, or if you could add something organic and absorbant that has no nutrition (something similar to coir maybe) to your substrate, that might work too?


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OfflineRaadt
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Re: wild P. mexicana - finally some nice growth! :) [Re: Olgualion]
    #1390225 - 03/19/03 04:26 AM (21 years, 4 months ago)

I was actually thinking the exact same thing, seeing as stones are usually found in a casing layer (non-nutritious).. it leads me to believe if you added a large ammount of non-nutritious substrate you might have higher stone formation levels. Marbles would probably work well, or if you could add something organic and absorbant that has no nutrition (something similar to coir maybe) to your substrate, that might work too?


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-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--

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InvisibleOlgualion
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Re: wild P. mexicana - finally some nice growth! :) [Re: Raadt]
    #1390481 - 03/19/03 05:55 AM (21 years, 4 months ago)

The next time dude cooks up some bags, I will ask him to try this. I feel that a bag of r.g.s. has a max potential yield depending upon the substrate (obviously). If the addition of non-nutritious 'barriers' give more points for sclerotia to begin, I can see the turn around time to reach this potential being greatly reduced.

I am calling them barriers, because IMO what the mycelia is doing is colonising substrate, then once the growing mycelia reaches these obstacles, it thinks 'hey, since I can't grow out anymore, I will put my energy into producing stones'


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Edited by Olgualion (03/20/03 02:44 AM)

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InvisibleOlgualion
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Re: wild P. mexicana - finally some nice growth! :) [Re: Olgualion]
    #1393897 - 03/20/03 02:44 AM (21 years, 4 months ago)

Thoughts anyone?


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Offlinekayaman9
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Re: wild P. mexicana - finally some nice growth! :) [Re: Olgualion]
    #1396724 - 03/21/03 12:48 AM (21 years, 4 months ago)

Just wondering if that is from the wild print I sent you? .

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InvisibleOlgualion
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Re: wild P. mexicana - finally some nice growth! :) [Re: kayaman9]
    #1396900 - 03/21/03 02:25 AM (21 years, 4 months ago)

No, this came from a nice trade with someone south of the border.

I'm going to PM you.


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InvisibleOlgualion
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Re: wild P. mexicana - finally some nice growth! :) [Re: zeronio]
    #1403555 - 03/23/03 01:39 PM (21 years, 4 months ago)

I was looking through some of my old posts, and came across a post with this pic


I now remember that this plate grew for a while then all of a sudden started getting black contamination that would quickly envelope the mycelium upon sporulation. The pictured plate is (i think) 2 transfers ago. It was an attempt to make an isolate from what dude and I thought to be a stone. If I remember correctly it came from a jar which was innoculated with a wedge. I had thought the culture was clean at that point, but subsequently found out that there was a hiding contam. The stone/ growth was halved, washed in H2O and placed onto agar in two different plates. The one plate contam'ed and this is the other. The only way this could be anything other than P. mexicana is if it is contaminated with P. cub. Which I suppose is possible. But I would think remotely.

Anyway, dude innoced a few jars with wedges, and they are nearing 50% colonisation after 6 days. He will probably shake for the second and final time tomorrow. I am getting all anxious to see how they will fruit.

Watch them be cubies now... lol


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