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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: Can condensation on the petri dish cause bacterial contamination?
    #84387 -

Hey. Yeah I experienced the same thing. Here's what to do. Tape the outside edges of your dishes with electrical tape or some other tape that will seal and covers the lip of the dish on each side. I took a roll of cling wrap and sawed off 1.5" pieces, rolls made this way work great. Secondly, consider spraying your area with Lysol, your contamination being at the edges of the dish indicates airborne contaminants in your grow area.

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Re: Can condensation on the petri dish cause bacterial contamination?
    #84389 -

Bacteria love wet conditions, they spread much more easily in fluids than on solids, as they are motile. It wont neccesarilly CAUSE the contamination, but it would facilitate it's rapid infestation of the plate.

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Re: Can condensation on the petri dish cause bacterial contamination?
    #84390 -

Are you inoculating with a syringe? I have heard reports that using a syringe on agar doesn't work well. Usually a clear slime develops with no spore germination. If you are using prints with the same problem, try an antibiotic media.


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Re: Can condensation on the petri dish cause bacterial contamination?
    #84391 -

Thank you, seniors!!!
I'm going to use your advices and let you know if it work out.

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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