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Anonymous

Re: Can condensation on the petri dish cause bacterial contamination?
    #84387 - 04/03/00 03:08 AM (22 years, 6 months ago)

Hey. Yeah I experienced the same thing. Here's what to do. Tape the outside edges of your dishes with electrical tape or some other tape that will seal and covers the lip of the dish on each side. I took a roll of cling wrap and sawed off 1.5" pieces, rolls made this way work great. Secondly, consider spraying your area with Lysol, your contamination being at the edges of the dish indicates airborne contaminants in your grow area.


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OfflineDan
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Registered: 05/12/01
Posts: 187
Last seen: 20 years, 10 months
Re: Can condensation on the petri dish cause bacterial contamination?
    #84389 - 04/03/00 09:35 AM (22 years, 6 months ago)

Bacteria love wet conditions, they spread much more easily in fluids than on solids, as they are motile. It wont neccesarilly CAUSE the contamination, but it would facilitate it's rapid infestation of the plate.

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OfflineWorkmanV
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Registered: 03/01/01
Posts: 3,566
Loc: Washington, USA
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Re: Can condensation on the petri dish cause bacterial contamination?
    #84390 - 04/03/00 07:52 AM (22 years, 6 months ago)

Are you inoculating with a syringe? I have heard reports that using a syringe on agar doesn't work well. Usually a clear slime develops with no spore germination. If you are using prints with the same problem, try an antibiotic media.


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Anonymous

Re: Can condensation on the petri dish cause bacterial contamination?
    #84391 - 04/04/00 03:19 AM (22 years, 6 months ago)

Thank you, seniors!!!
I'm going to use your advices and let you know if it work out.

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BOOM!



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