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old timer
Registered: 01/26/00
Posts: 702
Loc: VT
bacterial contamination and strain degeneration...
    #616321 - 04/23/02 11:44 AM (21 years, 10 months ago)

Hey all...Been AWOL from here for a little bit...but I am back.
Anyways let me get to the point...my cat is no stranger to birdseed and other grains or how to work with agar as she has been using those methods almost exclusively for the last few years....but recently my cat has had two very serious problems...
1) Out of no where lots of bacterial contamination started hitting her jars...no change in location of storage, no change in grain type, whether the jars where innoculated by a grain transfer or via agar wedge (both in a flowhood), no change in brand of grain. I am very confused what might have brought this sudden bacterial anal probe up. any thoughts or if you want more details on what my cat has been doing let me know via posting or PMs. also the only other thing i can think of is that maybe the temps have been a little higher than normal and on occasion spiked in the high 80s to 90 range.
anythoughts are always apreciated, many thanks

2) now the strains have been going for a while based off grain to grain transfers and also agar innoculations, no new agar had been made by my cat for the last few months....and she had been using stored cultures from her fridge (could this be the source? she has been using this fridge for along time without any problems) could the age of the culture be part of the problem? could the amount the strain was grain to grained be a problem?

again many thanks, ar393 and cat.

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myco contractor
Registered: 09/19/01
Posts: 2,417
Re: bacterial contamination and strain degeneration... [Re: ar393]
    #616343 - 04/23/02 12:17 PM (21 years, 10 months ago)

rinse the grain with H2O2 after steeping lowers bacterial contams according to the fire ants in my backyard

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Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 15 days, 5 hours
Re: bacterial contamination and strain degeneratio [Re: ar393]
    #616400 - 04/23/02 01:11 PM (21 years, 10 months ago)

Before you can fix the problem you will need to find the source. Prepare a few plates (petri dishes) with non-antibiotical agar and incubate for a few days. You shouldn't see any growth. If you do see bacterial growth you have a starting place to look... either inside your incubator or the agar itself, etc.

Assuming you didn't get any bacterial growth from the above step, take two plates and prepare to do a transfer. Only transfer to one of the plates. Repeat the same steps on the second plate, but do not actually do the transfer. Again, incubate and look for contamination. By comparing the two plates you can tell if the contamination came from the source of the transfer or during the transfer.

More or less, repeat the above steps with each phase of your grow operation. Eventually you will figure out where the bacteria is coming from. Once that is establishes you can work on cleaning that part of your system.

Remember, it is a lot easier to keep bacteria from showing up in the first place than it is to get rid of it after you have picked it up. You might need to toss your jars in the trash and buy new ones. You might also check your filter disks (if you are using/reusing them). If you are using a flow hood, check the hepa and the seals around it as well.

Good luck!

Just another spore in the wind.

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