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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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H2O2
    #8015754 -

I was wondering if anybody ever used peroxidated water for substrate in any form. I know some things people don't sterilize, I'm sure even with fairly mycelium selective substrates, there us some risk level of contamination. Could you not maybe hydrate it with a 10% H2O2 solution? Just a thought.


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Our quest for discovery fuels our creativity in all fields, not just science. If we reached the end of the line, the human spirit would shrivel and die. But I don't think we will ever stand still: we shall increase in complexity, if not in depth, and shall always be the center on an expanding horizon of possibilities.
-Stephen Hawking

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Re: H2O2 [Re: mr_minds_eye]
    #8016234 -

> I was wondering if anybody ever used peroxidated water for substrate in any form.

There's about 10,000 threads on this topic. Peroxide is not good for myc and stunts it's growth, it also only kills organisms without a peroxidase gene.


-FF

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Re: H2O2 [Re: fastfred]
    #8016511 -

Well I'm sorry to make it 10,001 but I have never seen a post on it being used anywhere besides agar and perlite. I'm not doubting you. I just haven't seen it.
I have had a little bit of mycelium fall into a heavily peroxidated solution in my perlite and not only manage to grow a bit, but fruit as well. I thought that after an initial period of acclimation, it grew just fine on it. I don't see any growth difference between my peroxidated and unperoxidated plates. That's just where I was coming from. I'm sorry to clutter the forums with a useless redundant post.


--------------------
Our quest for discovery fuels our creativity in all fields, not just science. If we reached the end of the line, the human spirit would shrivel and die. But I don't think we will ever stand still: we shall increase in complexity, if not in depth, and shall always be the center on an expanding horizon of possibilities.
-Stephen Hawking

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Re: H2O2 [Re: mr_minds_eye]
    #8020410 -

It's OK. It's just one of the most discussed and debated topics on the board.

Some people use a lot of it all the time, then they go around claiming how great it is when they likely would have had just as good or better success without it. It's like a healthy person paying $1 for a sugar pill and then claiming it as a source of their good health.

The only real use for peroxide that I know of is in treating cobweb mold. Most other fungal contams are resistant.

Some people use it as a surface sterilizer, but it still has the same problem in that it's not effective against many organisms. Bleach or iodine solutions are far better surface sterilizers for tissue and bleach and alcohol solutions are far better sterilizers for non-living surfaces.

As far as peroxidated agar... H2O2 is quickly degraded by heat and light. It also is quickly consumed by it's oxidizing action on organic materials. So I don't see how any peroxide could really survive being made into media. Even if added after sterilization it will still have to survive 50C until it cools and somehow not be consumed by reactions with organic elements in the media.

The same is true with it's use on substrates, etc.. It is quickly consumed and I don't see how short contact with a mild oxidizer will have any great activity on undesired agents, many of which will be completely resistant in the first place.

It's use in perlite is useful though. It kills spores and can reduce the microbial load in your water. It also breaks down into oxygen, which is also useful.

Otherwise peroxide is just sloppy technique with questionable utility IMHO. Any oxidizer is harmful to living things and will stunt their growth, regardless of peroxidase activity. Hyphal tips and unprotected shoots of myc are especially sensitive. When you hit myc with peroxide you'll usually see graying and a generally unhealthy appearance. IMO that is all your most exposed portions dying off and being oxidized away before the organism responds by producing more peroxidase.

That said, I certainly use peroxide for some things. It works well to sterilize tissue surfaces when you are trying to culture tissue rather than spores that might be deposited on that tissue. It's also great for keeping water/perlite fresher and less scummy. And it treats cobweb well. Other than that it's uses are pretty limited and people using it for everything are just adding a false sense of security and stunting their growth.


-FF

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Re: H2O2 (moved) [Re: mr_minds_eye]
    #8034058 -

This thread was moved from Advanced Mycology.

Reason:
Moved to cultivation, not an advanced mycology topic.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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